Results 201 to 210 of about 277,643 (313)

Rapid Adaptive Evolution under Combination Therapy in <i>Klebsiella pneumoniae</i>. [PDF]

ACS Infect Dis
de Souza CM, Lee A, Martínez OF, Ning K, da Costa MC, Maximiano MR, Feitosa GC, Neiva Y, Campos M, Ramada M, Alencar S, Hancock REW, Franco OL.   +12 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Comparative chloroplast genomics of Cypripedioideae: structural divergence, adaptive evolution, and phylogenomic insights. [PDF]

Front Plant Sci
Zhao X, Lan X, Wang Z, Li D, Sun W, Gai Y, Liu B.   +6 more
europepmc   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Mitochondrial genome assembly, comparative analysis of two caprifoliaceae species, and insights into adaptive evolution. [PDF]

BMC Plant Biol
Wang J, Ren M, Zhao M, Du H, Huang S, Wang S, Hou R, Jiang X, Yan C, Li J, Liu ZL.   +10 more
europepmc   +1 more source

The evolution of adaptive systems

, 2000
Brock, James P.
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Localized adaptive evolution in VP1 drives antigenic divergence of feline calicivirus despite high sequence conservation. [PDF]

Vet Res
Jiang Y, Qi Y, Ma B, Miao F, Yue H, Hou T, Chen T, Wang S, Li N, Zhang Y, Hu R, Zhang S.   +11 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Chromosome-Level Genome Assembly of <i>Morchella sextelata</i> Reveals Its Early Divergence and Adaptive Evolution. [PDF]

J Fungi (Basel)
Hong L, Fan Q, Tao N, Wang P, Liu P, Leng J, Yao C, Liu Q.   +7 more
europepmc   +1 more source