Results 111 to 120 of about 33,132 (307)

Applicability of mitotic figure counting by deep learning: a development and pan‐cancer validation study

FEBS Open Bio, EarlyView.
In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes, Maria X. Isaksen, Frida Julbø, Manohar Pradhan, Andreas Kleppe, Sepp De Raedt, Ole‐Johan Skrede, Turid Torheim, John Arne Nesheim, Hans Martin Mohn, Hanne A. Askautrud, Karolina Cyll, Wanja Kildal, Emma Rewcastle, Melinda Lillesand, Vebjørn Kvikstad, Emiel Janssen, Robert Jones, Odd Terje Brustugun, Bjørn Brennhovd, Erik Skaaheim Haug, Lill‐Tove Rasmussen Busund, Elin Richardsen, Sigve Andersen, Tom Dønnem, Kristina Lindemann, Gunnar Kristensen, Neil A. Shepherd, Marco Novelli, Knut Liestøl, David Kerr, Håvard E. Danielsen, Tarjei S. Hveem   +32 more
wiley   +1 more source

The Giant Magellan Telescope adaptive optics program

, 2014
The Giant Magellan Telescope (GMT) adaptive optics (AO) system will be an integral part of the telescope, providing laser guidestar generation, wavefront sensing, and wavefront correction to every instrument currently planned on the 25.4 m diameter GMT ...
Jose M. Filgueira, Pinna, Enrico, Bouchez, A, van Dam, Marcos Alejandro, Gelys Trancho, Simone Esposito, Antonin H. Bouchez, Daniele Gallieni, Acton, D. Scott, Biasi, Roberto, Roberto Biasi, D. S. Acton, Conan, Rodolphe, Fernando Santoro, Filgueira, Josema, Brady Espeland, Gallieni, Daniele, Santoro, Fernando, Brian A. McLeod, Marcos A. van Dam, McLeod, Brian A, Trancho, Gelys, Espeland, Brady, Rodolphe Conan, Enrico Pinna, Esposito, Simone   +25 more
core   +1 more source

A sodium laser guide star facility for the ANU/EOS space debris tracking adaptive optics demonstrator [PDF]

, 2014
The Australian National University and EOS Space Systems have teamed up to equip the EOS laser space debris tracking station on Mount Stromlo near Canberra, Australia, with sodium Laser Guide Star (LGS) Adaptive Optics (AO).
Rigaut, Francois, Nicolas Paulin, Ian Ritchie, Francis Bennet, Smith, C, Ian Price, Celine D'Orgeville, Craig Smith, Francois Rigaut, Brister, R, Blundell, Mark, Wang, Yanjie, Rod Brister, Amy Chan, Chan, Amy, Murray Dawson, Price, Ian, d'Orgeville, Celine, Sellars, Matthew, Kristina Uhlendorf, Dawson, Murray, Bennet, Francis, Uhlendorf, Kristina, Matt Sellars, Yanjie Wang, Yue Gao, Ritchie, Ian, Mark Blundell, Gao, Yue, Paulin, Nicolas   +29 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

The numerical simulation tool for the MAORY multiconjugate adaptive optics system [PDF]

, 2016
The Multiconjugate Adaptive Optics RelaY (MAORY) is and Adaptive Optics module to be mounted on the ESO European-Extremely Large Telescope (E-ELT).
XOMPERO, MARCO, M. Xompero, FOPPIANI, ITALO, S. Oberti, Cosentino, Giuseppe, G. Cosentino, SCHREIBER, LAURA, M. Patti, ESPOSITO, Simone, DIOLAITI, EMILIANO, ARCIDIACONO, CARMELO, R. C. Butler, P. Ciliegi, PUGLISI, Alfio Timothy, Oberti, Sylvain, S. Esposito, AGAPITO, GUIDO, Feautrier, Philippe, LOMBINI, MATTEO, I. Foppiani, Butler, R. C., G. Agapito, L. Schreiber, A. Puglisi, BREGOLI, Giovanni, F. Cortecchia, CORTECCHIA, Fausto, P. Feautrier, G. Bregoli, CILIEGI, Paolo, M. Lombini, C. Arcidiacono, E. Diolaiti, Patti, Mauro   +33 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

TMT NFIRAOS: adaptive optics for the Thirty Meter Telescope [PDF]

, 2012
NFIRAOS is the first-light adaptive optics system planned for the Thirty Meter Telescope, and is being designed at the National Research Council of Canada's Herzberg Institute of Astrophysics. NFIRAOS is a laser guide star multi-conjugate adaptive optics
Joeleff Fitzsimmons, Glen Herriot, Corinne Boyer, Peter Byrnes, Malcolm Smith, Wang, Lianqi, Carlos Correia, Ivan Wevers, Fitzsimmons, Joeleff, Boucher, Marc-Andre, Brent Ellerbroek, Véran, Jean-Pierre, Lianqi Wang, Herriot, Glen, Gilles, Luc, Dan Kerley, Boyer, Corrine, Jennifer Dunn, Caputa, Kris, Ellerbroek, Brent, Hill, Alexis, Reshetov, Vlad, Andersen, David, Hickson, Paul, Marc-André Boucher, John Pazder, Boucher, Marc-Andr\ue9, Paul Hickson, Luc Gilles, Byrnes, Peter, Jenny Atwood, Kerley, Dan, Kris Caputa, Jean-Pierre Véran, Wevers, Ivan, Correia, Carlos, V\ue9ran, Jean-Pierre, Alexis Hill, Scott Roberts, Atwood, Jenny, Pazder, John, Dunn, Jennifer, Boyer, Corinne, Roberts, Scott, Smith, Malcolm, David Andersen, Vlad Reshetov   +46 more
core   +1 more source

Correlated Photon Lidar Based on Time-Division Multiplexing

Photonics
Single-photon lidar (SPL) exhibits high sensitivity, making it particularly suitable for detecting weak echoes over long distances. However, its susceptibility to background noise necessitates the implementation of advanced filtering techniques and ...
Yun Jiang, Bo Liu, Zixun Wang, Fengyun Huang, Taibei Liu, Lan Luo, Feifan He, Yongqi Yang, Bin Zhao   +8 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source