Results 101 to 110 of about 18,252,740 (310)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Research Progress of Deformable Mirror and Its Control Algorithm

open access: yesGuangtongxin yanjiu
【Objective】Deformable Mirror(DM) is a key device in adaptive optics system for wavefront correction, and its performance directly determines the wavefront correction capability of the system.
LIANG Jingyuan   +7 more
doaj  

A Framework for Iterative Phase Retrieval Technique Integration into Atmospheric Adaptive Optics—Part II: High Resolution Wavefront Control in Strong Scintillations

open access: yesPhotonics
In this paper, we introduce atmospheric adaptive optics (AO) system architectures that utilize scintillation-resistant wavefront sensors based on iterative phase retrieval (IPR) techniques (described in detail in Part I) for closed-loop mitigation of ...
Mikhail A. Vorontsov, Ernst Polnau
doaj   +1 more source

A sodium laser guide star facility for the ANU/EOS space debris tracking adaptive optics demonstrator [PDF]

open access: yes, 2014
The Australian National University and EOS Space Systems have teamed up to equip the EOS laser space debris tracking station on Mount Stromlo near Canberra, Australia, with sodium Laser Guide Star (LGS) Adaptive Optics (AO).
Rigaut, Francois   +29 more
core   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source

TMT NFIRAOS: adaptive optics for the Thirty Meter Telescope [PDF]

open access: yes, 2012
NFIRAOS is the first-light adaptive optics system planned for the Thirty Meter Telescope, and is being designed at the National Research Council of Canada's Herzberg Institute of Astrophysics. NFIRAOS is a laser guide star multi-conjugate adaptive optics
Joeleff Fitzsimmons   +46 more
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

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