Results 101 to 110 of about 21,086 (310)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

MAVIS Adaptive Optics Module: optical configuration and expected performance

, 2022
The MCAO Assisted Visible Imager and Spectrograph (MAVIS) is currently in preliminary design for the ESO VLT. The instrument will provide multi-conjugate adaptive optics correction over a wide field of 30”x30”, feeding the visible part of the spectrum ...
Chinellato, Simonetta, Rigaut, François, Demetrio Magrin, David Brodrick, Simonetta Chinellato, Stefan Ströbele, Pinna, Enrico, Davide Greggio, Maria Bergomi, Aliverti, Matteo, Vaughn, Israel, Magrin, Demetrio, Israel Vaughn, Ströbele, Stefan, Kalyan Radhakrishnan, Guido Agapito, Brodrick, David, Cranney, Jesse, Taylor, Brian, Christian Schwab, Bergomi, Maria, Brian W. Taylor, Bonaglia, Marco, Greggio, Davide, Marafatto, Luca, Matteo Aliverti, Agapito, Guido, Marco Bonaglia, Valentina Viotto, Luca Marafatto, Schwab, Christian, Jesse Cranney, Radhakrishnan, Kalyan, Enrico Pinna, Viotto, Valentina, François Rigaut   +35 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Ultrahigh-speed multimodal adaptive optics system for microscopic structural and functional imaging of the human retina. [PDF]

Biomed Opt Express, 2022
Liu Z, Zhang F, Zucca K, Agrawal A, Hammer DX.   +4 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Research Progress of Deformable Mirror and Its Control Algorithm

Guangtongxin yanjiu
【Objective】Deformable Mirror(DM) is a key device in adaptive optics system for wavefront correction, and its performance directly determines the wavefront correction capability of the system.
LIANG Jingyuan, WANG Hairong, ZHANG Na, ZHANG Xiaodan, ZHAO Li, WANG Huiqing, WANG Yi, KE Xizheng   +7 more
doaj  

A Framework for Iterative Phase Retrieval Technique Integration into Atmospheric Adaptive Optics—Part II: High Resolution Wavefront Control in Strong Scintillations

Photonics
In this paper, we introduce atmospheric adaptive optics (AO) system architectures that utilize scintillation-resistant wavefront sensors based on iterative phase retrieval (IPR) techniques (described in detail in Part I) for closed-loop mitigation of ...
Mikhail A. Vorontsov, Ernst Polnau
doaj   +1 more source

Vision with spatial light modulator simulating multifocal contact lenses in an adaptive optics system. [PDF]

Biomed Opt Express, 2021
Vedhakrishnan S, Vinas M, Aissati S, Marcos S.   +3 more
europepmc   +1 more source

Status of the ARGOS ground layer adaptive optics system [PDF]

, 2012
ARGOS the Advanced Rayleigh guided Ground layer adaptive Optics System for the LBT (Large Binocular Telescope) is built by a German-Italian-American consortium. It will be a seeing reducer correcting the turbulence in the lower atmosphere over a field of
Brusa, G., Diethard Peter, Lewis, J., Connot, C., Sebastian Rabien, Raab, W., Martin Kulas, Simone Esposito, Pete Hubbard, Ihle, S., Peter, D., Vaitheeswaran, V., Wolfgang Gässler, Joar Brynnel, Buschkamp, P., Guido Brusa, Hans Gemperlein, Jason Lewis, Loose, C., Luca Carbonaro, Gässler, W., Nussbaum, E., Thomas Bluemchen, Haug, M., Brynnel, J., Edmund Nussbaum, Michael Lloyd-Hart, Christian Schwab, Borelli, J. L., Gilles Orban de Xivry, Busoni, L., Sebastian Ihle, Richard Davies, Richard Green, Matt Rademacher, Davies, R., José Luis Borelli, Marcus Haug, Beckmann, U., Christina Loose, Orban De Xivry, Gilles, Rademacher, M., Marco Bonaglia, Victor Gasho, Orban de Xivry, G., Gemperlein, H., Bluemchen, T., Noenickx, J., Lederer, R., Lehmitz, M., Rabien, S., Deysenroth, M., Durney, O., Jamison Noenickx, Borelli, J., Storm, J., Jesper Storm, Olivier Durney, Kulas, M., Lothar Barl, Reinhard Lederer, Bonaglia, M., Schwab, C., Matthias Deysenroth, Walfried Raab, Claus Connot, Green, R., Andreas Quirrenbach, Lorenzo Busoni, Lloyd-Hart, M., Peter Buschkamp, Michael Lehmitz, Esposito, S., Gasho, V., Ziegleder, J., Carbonaro, L., Vidhya Vaitheeswaran, Barl, L., Quirrenbach, A., Hubbard, P., Julian Ziegleder, Udo Beckmann   +81 more
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source