Identification, Heterologous Expression, and Functional Characterization of Bacillus subtilis YutF, a HAD Superfamily 5'-Nucleotidase with Broad Substrate Specificity. [PDF]
PLoS ONE, 2016 5'-nucleotidases (EC 3.1.3.5) catalyze the hydrolytic dephosphorylation of 5'-ribonucleotides and 5'-deoxyribonucleotides as well as complex nucleotides, such as uridine 5'-diphosphoglucose (UDP-glucose), nicotinamide adenine dinucleotide and flavin ...
Natalia P Zakataeva +5 more
doaj +1 more source
Mechanisms for DNA Charge Transport [PDF]
, 2010 DNA charge transport (CT) chemistry has received considerable attention by scientific researchers over the past 15 years since our first provocative publication on long range CT in a DNA assembly.1,2 This interest, shared by physicists, chemists and ...
Barton, Jacqueline K. +1 more
core +2 more sources
Emerging Therapeutic Strategies for Hearing Loss
Advanced Therapeutics, EarlyView.Challenges still exist in treating hearing loss in cases of severe damage to hair cells or spiral ganglion neurons. Here, a schematic diagram of cochlear sensory hair cells and auditory nerves is presented. It is found that in normal Corti organs, hair cells have upright stereocilia at the top and auditory neurons at the base. Ototoxic drugs, noise, or
Shanying Han +9 more
wiley +1 more source
PLoS ONE, 2014 Gene silencing and RNA interference are major cellular processes that control gene expression via the cleavage of target mRNA. Eukaryotic translation initiation factor 2C2 (EIF2C2, Argonaute protein 2, Ago2) is considered to be the major player of RNAi ...
Mahmoud Kandeel +7 more
doaj +1 more source
Molecular dynamics simulation study of the binding of purine bases to the aptamer domain of the guanine sensing riboswitch [PDF]
, 2009 Riboswitches are a novel class of genetic control elements that function through the direct interaction of small metabolite molecules with structured RNA elements.
Stock, Gerhard +2 more
core +1 more source
Self‐Adjusting Engineered Probiotic for Targeted Tumor Colonization and Local Therapeutics Delivery
Advanced Science, EarlyView.Zou et al. developed an engineered strain of Escherichia coli Nissle 1917 with precise targeting abilities for colonization and on‐demand payload release. The engineered probiotic survives and embolizes tumors only in the presence of more than 5 mM L‐lactate. Furthermore, the introduction of an α‐hemolysin circuit enhances its anti‐tumor effect.
Zhen‐Ping Zou +8 more
wiley +1 more source
Statistical distributions of sequencing by synthesis with probabilistic nucleotide incorporation [PDF]
Journal of Computational Biology. June 2009, 16(6): 817-827, 2014 Sequencing by synthesis is used in many next-generation DNA sequencing technologies. Some of the technologies, especially those exploring the principle of single-molecule sequencing, allow incomplete nucleotide incorporation in each cycle. We derive statistical distributions for sequencing by synthesis by taking into account the possibility that ...
arxiv +1 more source
Influence of a fluorobenzene nucleobase analogue on the conformational flexibility of RNA studied by molecular dynamics simulations [PDF]
, 2006 Chemically modified bases are frequently used to stabilize nucleic acids, to study the driving forces for nucleic acid structure formation and to tune DNA and RNA hybridization conditions.
Engels, Joachim W. (Prof. Dr.) +1 more
core
Microbiome Single Cell Atlases Generated with a Commercial Instrument
Advanced Science, EarlyView.Single cell sequencing is valuable for dissecting complex systems and uncovering unique features in individual cell types. However, tools for single cell microbial analysis are limited. Easily Accessible Single microbe sequencing (EASi‐seq), which adapts a commercially available single cell platform for efficient sequencing of single microbial genomes ...
Xiangpeng Li +8 more
wiley +1 more source
Remote Control of Eukaryotic Gene Expression by a Modular Ultrasound‐Responsive RNA Toolkit
Angewandte Chemie, EarlyView.A modular RNA‐based sonogenetic toolkit enables remote control of gene expression in eukaryotes by releasing small molecule modulators from ultrasound‐responsive carriers. These modulators activate engineered mRNA elements such as riboswitches, leading to tunable protein expression.
Fahimeh Charbgoo +7 more
wiley +2 more sources