Results 31 to 40 of about 73,809 (216)

From Cell‐Derived Vesicles to Hybrid Nanovectors: Biological Membranes as Functional Blueprints for Gene Delivery

Advanced Healthcare Materials, EarlyView.
Biologically derived and hybrid nonviral nanovectors are examined as distinct but convergent design approaches. Integrating synthetic components with biologically functionalized membranes allows efficient interactions with complex cellular environments.
Clara Baldari, Claudia Leone, Gabriella Leccese, Claudia De Stradis, Alessandra Invidia, Concetta Quintarelli, Biagio De Angelis, Franco Locatelli, Giuseppe Gigli, Gabriele Maiorano, Ilaria Elena Palamà   +10 more
wiley   +1 more source

Pharmacology of Recombinant Adeno-associated Virus Production

Molecular Therapy: Methods & Clinical Development, 2018
Recombinant adeno-associated viral (rAAV) vectors have been used in more than 150 clinical trials with a good safety profile and significant clinical benefit in many genetic diseases.
Magalie Penaud-Budloo, Achille François, Nathalie Clément, Eduard Ayuso   +3 more
doaj   +1 more source

Phosphorylation‐Facilitated CKB Lactylation At K11 By GCN5 Enhances Creatine Kinase Activity and Mitigates Neuronal Damage After Cerebral Ischemia‐Reperfusion

Advanced Science, EarlyView.
This study uncovers hierarchical coordination between K11 lactylation and S199 phosphorylation of CKB in cerebral ischemia‐reperfusion injury. Such dual modifications potentiate CKB enzymatic function, remodel energy metabolism, alleviate oxidative stress and neuronal damage, and represent a viable therapeutic target for stroke treatment.
Chao Duan, Ruolin Zhang, Shihui Ding, Ying Feng, Tao Shen, Ziwei Wang, Wendai Bao, Ke Shui, Jun Qin, Jun Chen, Min Zhang, Zijing Ren, Dongmei Zhao, Xingrong Guo, Zhibing Ai, Xin Yang, Peiyang Zhou, Zhiqiang Dong   +17 more
wiley   +1 more source

Compensatory Mitophagy via NEDD4/HIF‐1α/BNIP3 Pathway Restrains EndMT and Renal Allograft Interstitial Fibrosis Induced by TNFα

Advanced Science, EarlyView.
The role of TNFα in the process of renal allograft interstitial fibrosis is complex and multifaceted. As it can promote renal allograft interstitial fibrosis by inducing mitochondrial dysfunction and EndMT, and also mediating compensatory mitophagy through the NEDD4–HIF‐1α–BNIP3 pathway, which clears damaged mitochondria and inhibits EndMT, thereby ...
Dengyuan Feng, Qinghuan Shen, Junqi Zhang, Jiawen Liu, Zeping Gui, Zijie Wang, Qianguang Han, Shuang Fei, Hao Chen, Li Sun, Jun Tao, Zhijian Han, Xiaobing Ju, Min Gu, Jianjian Zhang, Ruoyun Tan   +15 more
wiley   +1 more source

Effects of cellular prion protein on rapid eye movement sleep deprivation-induced spatial memory impairment

Journal of Integrative Neuroscience, 2019
The effects of cellular prion protein on rapid eye movement sleep deprivation-induced spatial memory impairment were investigated, and the related mechanisms explored.
Li Hu, Peng Li, Zhendong You, Zhaohuan Zhang, Hongyang Jin, Liuqing Huang   +5 more
doaj   +1 more source

Modulation of miR‐23b Wnt/β‐catenin Axis Strengthens Endothelial Barrier Properties

Advanced Science, EarlyView.
Early blood‐brain barrier (BBB) disruption contributes to stroke and CNS disease pathology. miR‐23b was identified as a regulator of BBB integrity in brain endothelial cells. Inhibition of miR‐23b enhanced barrier‐associated properties, promoted repair‐related signaling, and reduced BBB leakage in experimental stroke models, supporting further ...
Victor Anthony Martinez, Rose E. Presby, Sara Nakanishi, Pallavi Gaur, Ugur Akcan, Johannes Daugaard Krog, Iben Daugaard Krog, Lipi Das, David S. Johnson, Danny Jamoul, Douglas Jury, Brian Lawson, Jacob Körbelin, Lewis M. Brown, Vilas Menon, Dritan Agalliu, Irene Munk Pedersen   +16 more
wiley   +1 more source

Natural Adeno-Associated Virus Serotypes and Engineered Adeno-Associated Virus Capsid Variants: Tropism Differences and Mechanistic Insights

Viruses
Today, adeno-associated virus (AAV)-based vectors are arguably the most promising in vivo gene delivery vehicles for durable therapeutic gene expression. Advances in molecular engineering, high-throughput screening platforms, and computational techniques
Estrella Lopez-Gordo, Kyle Chamberlain, Jalish Mahmud Riyad, Erik Kohlbrenner, Thomas Weber   +4 more
doaj   +1 more source

AAV vectors accumulate in the pineal gland after injections into the brain or spinal cord

Molecular Therapy: Methods & Clinical Development, 2021
AAV vectors are being used extensively for gene-modifying therapies for neurological disorders. Here, we report the surprising discovery that injections of different AAVs into the brain, spinal cord, or cerebrospinal fluid (CSF) lead to robust ...
Oswald Steward, Aminata P. Coulibaly, Mariajose Metcalfe, Jamie M. Dam, Kelly M. Yee   +4 more
doaj   +1 more source

BAP31 Drives Cartilage Calcification through Disruption of Autophagosome–Lysosome Fusion in Osteoarthritis

Advanced Science, EarlyView.
Mechanical stress activates BAP31 in chondrocytes. BAP31 competes with ATG14 for binding to STX17, disrupting the STX17–ATG14 complex required for autophagosome–lysosome fusion. The resultant autophagic flux blockade drives the generation of autophagy‐derived exosomes, which mediate pathological cartilage calcification in OA. Chondrocyte‐targeted BAP31
Zhi‐hua Xu, Ruo‐xin Wang, Feng He, Qian Liu, Fu‐yin Li, Min Hui, Yu‐qian Shi, Yu Jiang, Qing‐hua Li, Jun‐wen Jing, Shi‐bin Yu, Li‐tian Ma, Qian‐nan Niu, Mian Zhang   +13 more
wiley   +1 more source

AAV-based vectors for human diseases modeling in laboratory animals

Frontiers in Medicine
The development of therapeutic drugs and vaccines requires the availability of appropriate model animals that replicate the pathogenesis of human diseases. Both native and transgenic animals can be utilized as models.
Timur I. Aliev, Dmitry V. Yudkin
doaj   +1 more source