Results 181 to 190 of about 9,370,992 (242)
The Kaohsiung Journal of Medical Sciences, EarlyView.ABSTRACT N6‐methyladenosine (m6A) plays a critical role in osteoarthritis (OA) pathogenesis. This study investigates the role of the m6A reader insulin‐like growth factor 2 mRNA‐binding protein 3 (IGF2BP3) in OA progression. OA models were established in vivo through destabilization of the medial meniscus (DMM) surgery in mice and in vitro by ...
Xing‐Shi Yuan +8 more
wiley +1 more source
The Kaohsiung Journal of Medical Sciences, EarlyView.ABSTRACT Abnormal protein levels have been demonstrated to contribute to the progression of various diseases, including metabolic syndrome (MetS). Here, we speculated that the downregulation of BMI1 might lead to the failure of METTL3 degradation and contribute to METTL3 overexpression in MetS‐related liver injury.
Yun‐Jiang Chen +4 more
wiley +1 more source
Ion Activation Methods for Top‐Down Proteomics
Mass Spectrometry Reviews, EarlyView.ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley +1 more source
Mass Spectrometry Reviews, EarlyView.ABSTRACT Top‐down proteomics (TDP) characterizes proteoforms in cells, tissues, and biofluids, in discovery mode and on a global scale, requiring analytical tools with high peak capacity for proteoform separation and high sensitivity for proteoform detection, given the extremely high proteoform complexity and wide proteoform concentration dynamic range.
Guijie Zhu +5 more
wiley +1 more source
Recent Advances in Top‐Down Proteomics for Single‐Cell Research
Mass Spectrometry Reviews, EarlyView.ABSTRACT Individual cells have distinctive molecular characteristics, including biologically relevant proteoforms. Although single‐cell (SC) molecular omics offer unprecedented insights into cellular heterogeneity and function, the characterization of proteoforms in SCs remains an uncharted territory.
Jake A. Melby, Pei Su, Fabio P. Gomes
wiley +1 more source
Neuroprotection, EarlyView.Abstract Prime editing, a novel clustered regularly interspaced short palindromic repeats (CRISPR)‐based technology, fuses a reverse transcriptase (RT) to an engineered CRISPR‐associated protein 9 (Cas9) and uses a prime editing guide RNA (pegRNA)‐encoded template.
Tianshan Ji +4 more
wiley +1 more source
The role of tryptophan hydroxylase‐1 in metabolic dysfunction‐associated steatotic liver disease
Pediatric Investigation, EarlyView.Silencing hepatic tryptophan hydroxylase 1 (TPH1) alleviated diet‐induced steatotic liver disease by reducing lipid accumulation, oxidative stress, inflammation, fibrosis, and hepatocyte apoptosis. These protective effects were mediated, at least in part, through restoration of Parkin‐dependent mitochondrial autophagy, identifying hepatic TPH1 as a ...
Shuangzhen Jia +3 more
wiley +1 more source
Phytotherapy Research, EarlyView.PTA alleviates atherosclerosis by regulating the MDM2/GPX4 axis‐mediated endothelial ferroptosis. ABSTRACT Endothelial ferroptosis is a crucial pathogenic driver of atherosclerosis (AS) progression. Protosappanin A (PTA), a bioactive compound from Caesalpinia sappan L., protects cardiovascular vessels by regulating ferroptosis.
Jiamei Fu +6 more
wiley +1 more source
Phytotherapy Research, EarlyView.Neohesperidin alleviates doxorubicin‐induced cardiotoxicity by inhibiting METTL3‐mediated m6A modification of Nrf2 mRNA, thereby regulating Nrf2 and suppressing ferroptosis. ABSTRACT Doxorubicin (DOX) has antitumor efficacy, but its clinical application is restricted by multiple side effects, especially cardiotoxicity.
Ruijia Wen +9 more
wiley +1 more source
Adeno-Associated Virus (AAV) Vectors in Cancer Gene Therapy [PDF]
Journal of Controlled Release, 2016 Gene delivery vectors based on adeno-associated virus (AAV) have been utilized in a large number of gene therapy clinical trials, which have demonstrated their strong safety profile and increasingly their therapeutic efficacy for treating monogenic diseases.
David Schaffer +2 more
exaly +7 more sources