Results 71 to 80 of about 149,310 (262)

In Vitro Transcription of the Gal Operon Requires Cyclic Adenosine Monophosphate and Cyclic Adenosine Monophosphate Receptor Protein

Journal of Biological Chemistry, 1971
Abstract In a transcription system containing λpgal8 DNA as template and Escherichia coli RNA polymerase, the addition of cyclic adenosine monophosphate receptor protein (CRP) and 3',5'-cyclic AMP causes a 15-fold increase in gal mRNA synthesis. Gal mRNA was measured by hybridizing the [3H]RNA product to the separated strands of λpgal8 DNA after ...
S P, Nisseley, W B, Anderson, M E, Gottesman, R L, Perlman, I, Pastan   +4 more
openaire   +2 more sources

A Universal Nucleoside‐to‐Nucleoside‐5′‐Triphosphate Enzyme Cascade Driven by Polyphosphate

Angewandte Chemie International Edition, EarlyView.
Nucleoside‐5′‐triphosphates (NTPs) are essential components for the generation of nucleic acids, yet their chemical synthesis is hindered by the low yield of nucleoside phosphorylation. Here, we present a universal, two‐enzyme nucleoside‐to‐NTP cascade that is driven by polyphosphate.
Jonathan P. Suess, Nicolas V. Cornelissen   +1 more
wiley   +1 more source

Detection and characterisation of NAD(P)H-diaphorase activity in Dictyostelium discoideum cells (Protozoa)

European Journal of Histochemistry, 2012
In Dictyostelium discoideum (D. discoideum), compounds generating nitric oxide (NO) inhibit its aggregation and differentiation without altering cyclic guanosine monophosphate (cGMP) production.
A. Amaroli, M.G. Chessa
doaj   +1 more source

Characterization of the taste receptor‐related G‐protein, α‐gustducin, in pancreatic β‐cells

Journal of Diabetes Investigation, 2020
Aims/Introduction Taste receptors, T1rs and T2rs, and the taste‐selective G‐protein, α‐gustducin, are expressed outside the taste‐sensing system, such as enteroendocrine L cells.
Haruhide Udagawa, Masaki Hiramoto, Miho Kawaguchi, Takashi Uebanso, Mica Ohara‐Imaizumi, Takao Nammo, Wataru Nishimura, Kazuki Yasuda   +7 more
doaj   +1 more source

Dietary Glutamine Supplementation Alleviated Rumen Epithelium Oxidative Damage and Apoptosis Induced by Feed Restriction via Maintaining Mitochondrial Homeostasis in Female Yaks

Animal Research and One Health, EarlyView.
Dietary glutamine (Gln) supplementation increased the ATP levels and promoted cell proliferation in the rumen epithelium of feed‐restricted yaks. Besides, Gln inhibited ROS accumulation and reduced oxidative damage and apoptosis in the rumen epithelium of feed‐restricted yaks.
Ziqi Yue, Shanpeng Ke, Liyuan Shi, Rui Hu, Zhisheng Wang, Quanhui Peng, Huawei Zou, Jianxin Xiao, Yahui Jiang, Fali Wu, Yiping Tang   +10 more
wiley   +1 more source

Inosine‐Triphosphate‐Pyrophosphatase Activity as a Potential Predictor of Methotrexate Remission in Juvenile Idiopathic Arthritis

Arthritis &Rheumatology, EarlyView.
Objective Methotrexate (MTX) is the first‐line therapy for juvenile idiopathic arthritis (JIA), but up to 40% of patients do not respond to it. Low inosine triphosphate pyrophosphatase (ITPA) activity has been associated with reduced clinical remission. We investigated the role and underlying mechanisms of ITPA in vitro. Methods ITPA enzymatic activity
Sofia Sindici Forgiarini, Marianna Lucafò, Debora Curci, Davide Selvestrel, Valentina Moressa, Sofia Pagarin, Barbara Bellich, Martina Franzin, Alberto Tommasini, Mara L. Becker, Laura B. Ramsey, Susan D. Thompson, Carl D. Langefeld, Federica Corba, Edoardo Marrani, Gabriele Simonini, Gabriele Stocco, Giuliana Decorti, Andrea Taddio, Serena Pastore   +19 more
wiley   +1 more source

CAQ Corner: Basic concepts of transplant immunology

, 2022
Liver Transplantation, EarlyView.
Amanda Cheung, Josh Levitsky
wiley   +1 more source

Expert Perspectives: Defining and Managing Progressive Pulmonary Fibrosis in Systemic Sclerosis

Arthritis &Rheumatology, EarlyView.
Systemic sclerosis–associated interstitial lung disease (SSc‐ILD) is one of the leading causes of morbidity and mortality in SSc, affecting up to three‐quarters of patients. The disease course is highly heterogeneous, ranging from indolent, nonprogressive forms to rapidly progressive pulmonary fibrosis (PPF).
Devis Benfaremo, Dinesh Khanna, Corrado Campochiaro, Vincent Cottin, Fabrizio Luppi, Marco Matucci‐Cerinic, Gianluca Moroncini, Yannick Allanore   +7 more
wiley   +1 more source

AMPc: una molécula clave en los eventos de regulación inmune y en el control de la replicación del VIH cAMP: A keymolecule in events of immune regulation and in the control of HIV replication

Infectio, 2012
El monofosfato de adenosina cíclico (AMPc) induce la activación de la proteína cinasa A, la cual regula negativamente la activación, la proliferación celular y la producción de IL-2, en células T.
César Mauricio Rueda, Paula Andrea Velilla, Mauricio Rojas, María Teresa Rugeles   +3 more
doaj  

A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates

Biotechnology and Bioengineering, EarlyView.
Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm, Martin Pfeiffer, Johannes Zöhrer, Bernd Nidetzky   +3 more
wiley   +1 more source