Results 51 to 60 of about 2,621 (218)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Two-Stage Multi-Objective Optimization for Improving the Aerodynamic Characteristics of High-Speed Train Nose Shape

Fluids
An optimal high-speed train nose design was formulated to reduce aerodynamic drag, mitigate tunnel micro-pressure waves, and improve crosswind safety. Using the vehicle modeling function, the EMU-320 nose shape was derived and applied as the base model ...
Suhwan Yun, Minho Kwak, Hyungmin Kang, Wonhee Park, Taesoo Kwon   +4 more
doaj   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Transfer Optimization for Efficient Aerodynamic Shape Design

Aerospace
Constructing rapid aerodynamic shape optimization frameworks based on high-fidelity reduced-order models (ROMs) has become a mainstream solution for alleviating the excessive computational cost of full-order simulation-based design, especially for ...
Boda Zheng, Weigang Yao, Min Xu
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Data-driven aerodynamic shape optimization of rotor airfoils based on deep learning

Advances in Aerodynamics
As the fundamental component of rotor blades, the airfoil dominates the aerodynamic performance of helicopter rotors. The aerodynamic shape optimization of rotor airfoils is thus an effective means of improving their aerodynamic performance.
Jiaqi Liu, Rongqian Chen, Jinhua Lou, Yancheng You   +3 more
doaj   +1 more source

Aerodynamic Shape Optimization of a Missile Using a Multiobjective Genetic Algorithm

International Journal of Aerospace Engineering, 2020
The aim of this paper is to demonstrate the effects of the shape optimization on the missile performance at supersonic speeds. The N1G missile model shape variation, which decreased its aerodynamic drag and increased its aerodynamic lift at supersonic ...
Ahmet Şumnu, İbrahim Halil Güzelbey, Orkun Öğücü   +2 more
doaj   +1 more source