Results 101 to 110 of about 258,589 (319)

Against the Law: Indigenous Feminism and the Nation-State

open access: yes, 2011
The Native feminist theorizing advocated in thus article points to the importance of looking at Native organizing and Native Studies - and by extension ethnic studies as well - as going beyond an intellectual commitment to a politics of multicultural ...
Smith, Andrea
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

Dancing Through the Crisis

open access: yes, 2010
The financial crisis jeopardized life with unbridled risk. A radical imagination would require shifting what we would risk together to gain something altogether different.
Martin, Randy
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Praxis, Learning, and New Cooperativism in Venezuela: An Initial Look at Venezuela’s Socialist Production Units

open access: yes, 2010
In this paper, I address the question as to the extent to which the participatory and democratic processes taking place as part of Venezuela\u27s new cooperative movement can be said to be a component for the building of social relations that challenge ...
Larrabure, Manuel
core  

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

open access: yesFEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee   +6 more
wiley   +1 more source

Crossing Chris: Some Markerian Affinities

open access: yesImage & Narrative, 2010
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Adrian Martin
doaj  

Decomposition and Suburban Space

open access: yes, 2010
This essay provides a case-study of the development and decomposition of a number of organizing initiatives taking place in Long Island, New York during the late 1990s and early 2000s, and is particularly concerned with the relationship of these ...
Cash, Conor
core  

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

open access: yesFEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young   +7 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

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