Results 1 to 10 of about 2,570,021 (252)

Methods for Improving Aptamer Binding Affinity

Molecules, 2016
Aptamers are single stranded oligonucleotides that bind a wide range of biological targets. Although aptamers can be isolated from pools of random sequence oligonucleotides using affinity-based selection, aptamers with high affinities are not always ...
Hijiri Hasegawa, Nasa Savory, Koichi Abe, Kazunori Ikebukuro   +3 more
doaj   +3 more sources

Global protein-ligand binding affinity profiling via photocatalytic labeling [PDF]

Nature Communications
Protein-ligand binding, selectivity, and affinity dictate the effects of drugs and endogenous molecules in cells. Currently, potential protein-ligand interactions are identified by qualitative interpretation of proteomic, transcriptomic, or genomic data,
Charles D. Warren, Noah Yardeny, Siyang Peng, Colin S. Burdette, Jacob B. Geri   +4 more
doaj   +2 more sources

Low-affinity CTCF binding drives transcriptional regulation whereas high-affinity binding encompasses architectural functions

iScience, 2023
Summary: CTCF is a DNA-binding protein which plays critical roles in chromatin structure organization and transcriptional regulation; however, little is known about the functional determinants of different CTCF-binding sites (CBS).
Ester Marina-Zárate, Ana Rodríguez-Ronchel, Manuel J. Gómez, Fátima Sánchez-Cabo, Almudena R. Ramiro   +4 more
doaj   +1 more source

Learned features of antibody-antigen binding affinity

Frontiers in Molecular Biosciences, 2023
Defining predictors of antigen-binding affinity of antibodies is valuable for engineering therapeutic antibodies with high binding affinity to their targets.
Nathaniel L. Miller, Nathaniel L. Miller, Thomas Clark, Thomas Clark, Rahul Raman, Rahul Raman, Ram Sasisekharan, Ram Sasisekharan   +7 more
doaj   +1 more source

Probing high affinity sequences of DNA aptamer against VEGF165. [PDF]

PLoS ONE, 2012
Vascular endothelial growth factor (VEGF(165)) is a potent angiogenic mitogen commonly overexpressed in cancerous cells. It contains two main binding domains, the receptor-binding domain (RBD) and the heparin-binding domain (HBD). This study attempted to
Harleen Kaur, Lin-Yue Lanry Yung
doaj   +1 more source

Binding affinity and mechanisms of SARS-CoV-2 variants

Computational and Structural Biotechnology Journal, 2021
During the rapid worldwide spread of SARS-CoV-2, the viral genome has been undergoing numerous mutations, especially in the spike (S) glycoprotein gene that encode a type-I fusion protein, which plays an important role in the infectivity and ...
Yanqiang Han, Zhilong Wang, Zhiyun Wei, Igor Schapiro, Jinjin Li   +4 more
doaj   +1 more source

GANsDTA: Predicting Drug-Target Binding Affinity Using GANs

Frontiers in Genetics, 2020
The computational prediction of interactions between drugs and targets is a standing challenge in drug discovery. State-of-the-art methods for drug-target interaction prediction are primarily based on supervised machine learning with known label ...
Lingling Zhao, Junjie Wang, Long Pang, Yang Liu, Jun Zhang   +4 more
doaj   +1 more source

3pHLA-score improves structure-based peptide-HLA binding affinity prediction

Scientific Reports, 2022
Binding of peptides to Human Leukocyte Antigen (HLA) receptors is a prerequisite for triggering immune response. Estimating peptide-HLA (pHLA) binding is crucial for peptide vaccine target identification and epitope discovery pipelines.
Anja Conev, Didier Devaurs, Mauricio Menegatti Rigo, Dinler Amaral Antunes, Lydia E. Kavraki   +4 more
doaj   +1 more source

Interface-Based Design of High-Affinity Affibody Ligands for the Purification of RBD from Spike Proteins

Molecules, 2023
The outbreak of coronavirus disease 2019 (COVID-19) has sparked an urgent demand for advanced diagnosis and vaccination worldwide. The discovery of high-affinity ligands is of great significance for vaccine and diagnostic reagent manufacturing. Targeting
Siyuan Song, Qinghong Shi
doaj   +1 more source

Isothermal titration calorimetry determination of thermodynamics of binding of cocaine and its metabolites to humanized h2E2 anti-cocaine mAb

Biochemistry and Biophysics Reports, 2022
We analyzed the thermodynamics of binding of cocaine and several cocaine metabolites to a humanized anti-cocaine mAb (h2E2), which is under development for the treatment of cocaine use disorders, using isothermal titration calorimetry.
Terence L. Kirley, Andrew B. Norman
doaj   +1 more source