Results 11 to 20 of about 1,037,789 (311)

Multiplexed Affinity Measurements of Extracellular Vesicles Binding Kinetics

Sensors, 2021
Extracellular vesicles (EVs) have attracted significant attention as impactful diagnostic biomarkers, since their properties are closely related to specific clinical conditions.
Elisa Chiodi, George G. Daaboul, Allison M. Marn, M. Selim Ünlü   +3 more
doaj   +2 more sources

Distance plus attention for binding affinity prediction

Journal of Cheminformatics
Protein-ligand binding affinity plays a pivotal role in drug development, particularly in identifying potential ligands for target disease-related proteins.
Julia Rahman, M. A. Hakim Newton, Mohammed Eunus Ali, Abdul Sattar   +3 more
doaj   +2 more sources

Ensembling methods for protein-ligand binding affinity prediction

Scientific Reports
Protein-ligand binding affinity prediction is a key element of computer-aided drug discovery. Most of the existing deep learning methods for protein-ligand binding affinity prediction utilize single models and suffer from low accuracy and generalization ...
Jiffriya Mohamed Abdul Cader, M. A. Hakim Newton, Julia Rahman, Akmal Jahan Mohamed Abdul Cader, Abdul Sattar   +4 more
doaj   +2 more sources

Interface-Based Design of High-Affinity Affibody Ligands for the Purification of RBD from Spike Proteins

Molecules, 2023
The outbreak of coronavirus disease 2019 (COVID-19) has sparked an urgent demand for advanced diagnosis and vaccination worldwide. The discovery of high-affinity ligands is of great significance for vaccine and diagnostic reagent manufacturing. Targeting
Siyuan Song, Qinghong Shi
doaj   +1 more source

Isothermal titration calorimetry determination of thermodynamics of binding of cocaine and its metabolites to humanized h2E2 anti-cocaine mAb

Biochemistry and Biophysics Reports, 2022
We analyzed the thermodynamics of binding of cocaine and several cocaine metabolites to a humanized anti-cocaine mAb (h2E2), which is under development for the treatment of cocaine use disorders, using isothermal titration calorimetry.
Terence L. Kirley, Andrew B. Norman
doaj   +1 more source

Calreticulin Binding Affinity for Glycosylated Laminin [PDF]

Journal of Biological Chemistry, 1996
Several lines of evidence indicate that calreticulin has lectin-like properties. As a molecular chaperone, calreticulin binds preferentially to nascent glycoproteins via their immature carbohydrates; this property closely resembles that seen for calnexin, a chaperone with extensive molecular identity to calreticulin. A cell surface form of calreticulin
J M, McDonnell, G E, Jones, T K, White, M L, Tanzer   +3 more
openaire   +2 more sources

Guanosine nucleotides regulate B2 kinin receptor affinity of agonists but not of antagonists: Discussion of a model proposing receptor precoupling to G protein [PDF]

, 2000
The effect of nucleotides on binding of the B2 kinin (BK) receptor agonist {[}H-3]BK and the antagonist {[}H-3]NPC17731 to particulate fractions of human foreskin fibroblasts was studied.
Er Faussner, Faussner, Alexander, Adelbert A. Roscher, Roscher, Adelbert A.   +3 more
core   +1 more source

Statistical deconvolution of enthalpic energetic contributions to MHC-peptide binding affinity [PDF]

, 2006
Background: MHC Class I molecules present antigenic peptides to cytotoxic T cells, which forms an integral part of the adaptive immune response. Peptides are bound within a groove formed by the MHC heavy chain. Previous approaches to MHC Class I-peptide
Hattotuwagama, C.K., Drew, M.G.B., Moss, David S., Hattotuwagama, C.K., Davies, M.N., Davies, M.N., David S Moss, Drew, M.G.B., Channa K Hattotuwagama, Drew, Michael G.B., Flower, Darren R., Moss, D.S., Matthew N Davies, Hattotuwagama, Channa K., Davies, Matthew N., Michael GB Drew, Darren R Flower, Flower, D.R., Flower, D.R.   +18 more
core   +1 more source

How to measure and evaluate binding affinities

eLife, 2020
Quantitative measurements of biomolecule associations are central to biological understanding and are needed to build and test predictive and mechanistic models. Given the advances in high-throughput technologies and the projected increase in the availability of binding data, we found it especially timely to evaluate the current standards for ...
Pavanapuresan P Vaidyanathan, Ishraq AlSadhan, Inga Jarmoskaite, Daniel Herschlag   +3 more
openaire   +3 more sources

Multiplexed Small Molecule Ligand Binding Assays by Affinity Labeling and DNA Sequence Analysis

, 2021
Small molecule binding assays to target proteins are a core component of drug discovery and development. While a number of assay formats are available, significant drawbacks still remain in cost, sensitivity, and throughput.
Krusemark, Casey J., Casey, Krusemark, Bo, Cai, Cai, Bo   +3 more
core   +1 more source