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Sequential phosphoproteomic enrichment through complementary metal-directed immobilized metal ion affinity chromatography.

Analytical Chemistry, 2014
Methodologies to enrich heterogeneous types of phosphopeptides are critical for comprehensive mapping of the under-explored phosphoproteome. Taking advantage of the distinct binding affinities of Ga(3+) and Fe(3+) for phosphopeptides, we designed a metal-
Chia-Feng Tsai   +9 more
semanticscholar   +1 more source

Hydrophilic polydopamine-coated graphene for metal ion immobilization as a novel immobilized metal ion affinity chromatography platform for phosphoproteome analysis.

Analytical Chemistry, 2013
To discover trace phosphorylated proteins or peptides with great biological significance for in-depth phosphoproteome analysis, it is urgent to develop a novel technique for highly selective and effective enrichment of phosphopeptides.
Yinghua Yan   +5 more
semanticscholar   +1 more source

Flow chemistry synthesis of zolpidem, alpidem and other GABAA agonists and their biological evaluation through the use of in-line frontal affinity chromatography

, 2013
The flow of information between chemical and biological research can present a bottleneck in pharmaceutical research. Tools that bridge these disciplines and aid information exchange have therefore clear value.
Lucie Guetzoyan   +3 more
semanticscholar   +1 more source

Small-molecule-based affinity chromatography method for antibody purification via nucleotide binding site targeting.

Analytical Chemistry, 2012
The conserved nucleotide binding site (NBS), found within the Fab variable domain of antibodies, remains a not-so-widely known and underutilized site.
Nathan J. Alves   +5 more
semanticscholar   +1 more source

Affinity chromatography: a valuable strategy to isolate substrates of methionine sulfoxide reductases?

Antioxidants and Redox Signaling, 2012
Reactive oxygen species fulfill key roles in development and signaling, but lead at high concentration to damage in macromolecules. In proteins, methionine (Met) is particularly prone to oxidative modification and can be oxidized into Met sulfoxide (MetO)
L. Tarrago   +6 more
semanticscholar   +1 more source

Affinity Chromatography

Separations Technology, 2020
W. P. Olson
semanticscholar   +2 more sources

Latest Trends on the Future of Three-Dimensional Separations in Chromatography

Chemical Reviews, 2021
Peter J Schoenmakers   +2 more
exaly  

O-carboxymethyl chitosan Schiff base complexes as affinity ligands for immobilized metal-ion affinity chromatography of lysozyme.

Journal of Chromatography A, 2018
Ö. Acet   +6 more
semanticscholar   +1 more source

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