Results 41 to 50 of about 1,096,305 (236)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

IN VITRO COMPARISON OF DISK DIFFUSION METHOD AND AGAR DILUTION METHOD FOR SENSITIVITY OF POLYMYXIN B AGAINST MULTI DRUG RESISTANT ACINETOBACTER BAUMANNII

Pakistan Armed Forces Medical Journal, 2019
Objective: To compare the in vitro disk diffusion method and agar dilution method for sensitivity of Polymyxin B against multi drug resistant Acinetobacter baumannii. Study Design: Comparative cross sectional. Place and Duration of Study: The study
Fatima Sana, Luqman Satti, Gohar Zaman, Aamer Ikram, Adeel Hussain Gardezi, Muhammad Tahir Khadim   +5 more
doaj   +4 more sources

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Exploring the influence of lactoferrin on wound healing and related signaling pathways: a systematic review

FEBS Open Bio, EarlyView.
Lactoferrin promotes wound healing by activating MAPK, PI3K/Akt, and extracellular matrix pathways, enhancing fibroblast remodeling, proliferation, and re‐epithelialization. These mechanisms highlight its therapeutic potential in inflammation control, tissue repair, and remodeling.
Morgana Lüdtke Azevedo, Kátia Cristiane Hall, Matheus dos Santos Fernandez, Geovanna Peter Corrêa, Rafael Guerra Lund   +4 more
wiley   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Dilution-Induced Formation of Hybrid Perovskite Nanoplatelets

, 2016
Perovskite nanocrystals (NCs) are an important extension to the fascinating field of hybrid halide perovskites. Showing significantly enhanced photoluminescence (PL) efficiency and emission wavelengths tunable through halide content and size, they hold ...
Yu Tong (311947), Carlos Cardenas-Daw (1511950), Jacek K. Stolarczyk (1485922), Willem Vanderlinden (1596412), Alexander S. Urban (1356375), Florian Ehrat (1661398), Lakshminarayana Polavarapu (1284246)   +6 more
core   +2 more sources

Pembuatan Dodol Jelly dengan Penambahan Agar Strip (Gracilaria sp.) di Kabupaten Karawang [PDF]

, 2020
Kabupaten Karawang merupakan salah satu daerah penghasil rumput laut jenis Gracilaria sp. Untuk meningkatkan nilai jualnya, rumput laut Gracilaria sp. dapat diolah menjadi agar strip. Agar strip Gracilaria sp.
Adi, Catur Pramono, Prasetyawati, Sukma Budi, Tanjung, Anasri, Saputra, Rahmad Surya Hadi   +3 more
core   +2 more sources

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Studies on antimicrobial activity, in vitro, of Physalis angulata L. (Solanaceae) fraction and physalin B bringing out the importance of assay determination

Memorias do Instituto Oswaldo Cruz, 2005
Complex physalin metabolites present in the capsules of the fruit of Physalis angulata L. have been isolated and submitted to a series of assays of antimicrobial activity against Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213, S ...
Melissa TG Silva, Sonia M Simas, Terezinha GFM Batista, Paola Cardarelli, Therezinha CB Tomassini   +4 more
doaj   +1 more source