Results 61 to 70 of about 483,306 (352)

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

Detection of biofilm formation among the mastitis isolates of Staphylococci by evaluation of three different screening methods

Kufa Journal for Veterinary Medical Sciences, 2011
Internalization and biofilm formation  is an important step in staphylococcal mastitis pathogenesis . Twenty  five staphylococcal isolates  of coagulase negative and coagulase positive  were collected from clinical mastitis in cows, and biofilm ...
Hadeel M. Fiadh
doaj   +1 more source

Evaluation of Biofilm Formation and Presence of Genes in Clinical Isolates [PDF]

Osong Public Health and Research Perspectives, 2018
Objectives Biofilm formation is one of the important features of Staphylococcus epidermidis, particularly in nosocomial infections. We aimed to investigate the biofilm production by phenotypic methods and the presence of ica genes in S epidermidis ...
Maryam Kord, Abdollah Ardebili, Maryam Jamalan, Roghaye Jahanbakhsh, Naser Behnampour, Ezzat Allah Ghaemi   +5 more
doaj   +1 more source

The safety of ready-to-eat meals under different consumer handling conditions : a thesis presented in partial fulfilment of the requirements for the degree of Master in Food Technology at Massey University, Manawatū, New Zealand [PDF]

, 2016
Microbial count is an important index to measure the safety status of a food. This trial aimed to determine the safety of eight meals (four meats and four vegetarians) by using the agar plate counting method to measure the populations of total bacteria ...
Jiang, Fan
core  

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Seagrass Posidonia oceanica (L.) Delile as a marine biomarker: A metabolomic and toxicological analysis [PDF]

, 2018
A human-made environmental disaster due to the shipwrecked of Costa Concordia cruise vessel on the Tuscan Island of Giglio (Italy) coast and the possible pollutants release has been feared, so requiring the activation of removal operations and the ...
Agostini, Ardizzone, Beck, Bitam, Boudouresque, Cannac, Dalla Via, Del Moral, Del Serrone, Di Sotto, Di Sotto, Di Sotto, Di Sotto, Dumay, Díaz, Farah, Fitzpatrick, Fonseca, Francour, Gacia, Gacia, Grace, Greco, Heglmeier, Jones, Kuo, Lassauque, Maestrini, Maggi, Moglia, Mollica, Montefalcone, Nicoletti, Nicoletti, Pérez, Reich, Richir, Ruiz, Sager, Toniolo   +39 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Multiple Syringe Inoculator for Agar Plates [PDF]

Applied and Environmental Microbiology, 1977
The design and operation of a manually operated multiple syringe inoculator was described. Either 9 or 21 inoculations of constant volume could be made simultaneously. Up to 100 plates could be inoculated in 15 min with excellent reproducibility. No contact occurred between the inoculating needles and the agar surface.
T, Kaneko, M, Holder-Franklin, M, Franklin   +2 more
openaire   +2 more sources

Development and application of an assay for uranyl complexation by fungal metabolites, including siderophores [PDF]

, 2003
An assay to detect UO2 2+ complexation was developed based on the chrome azurol S (CAS) assay for siderophores (B. Schwyn and J. B. Neilands, Anal. Biochem.
Collison, David, Halliday, Verity, Livens, Francis R., Renshaw, Joanna C., Robson, Geoffrey D., Taylor, Robin J., Trinci, Anthony P J, Wiebe, Marilyn G.   +7 more
core   +2 more sources

Alcohol‐induced altered glycans in human tracheal epithelial cells promote bacterial adhesion

FEBS Open Bio, EarlyView.
Alcohol induces altered glycans to promote bacteria adhesion. Heavy alcohol drinking is known to increase the risk of bacterial pneumonia. However, the link between alcohol levels and risk of infection remains underexplored. Recently, we found that alcohol induced α2‐6sialo mucin O‐glycans in human tracheobronchial epithelial cells, which mediated the ...
Pi‐Wan Cheng, Souvik Datta, Derrick R. Samuelson   +2 more
wiley   +1 more source