Results 161 to 170 of about 315,422 (280)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

A critical appraisal of urolithiasis clinical practice guidelines using the AGREE II instrument. [PDF]

Transl Androl Urol, 2023
Zou B, Zhou Y, He Z, Zhou X, Dong S, Zheng X, Xu R, Duan X, Zeng G.   +8 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Quality assessment of clinical practice guidelines in Kenya using the AGREE II tool: a methodological review. [PDF]

BMJ Open, 2023
Sagam CK, Were LM, Otieno JA, Mulaku MN, Kariuki S, Ochodo E.   +5 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Clinical practice guidelines and quality standards for early intervention in psychosis: an AGREE II appraisal and systematic review of service components. [PDF]

Front Psychiatry
Scognamiglio P, Martiadis V, Diaspro G, Viacava L, Longobardi A, Rea R, Iniziato V, Saviano P, La Pia S.   +8 more
europepmc   +1 more source

Is AGREE II a counsel of perfection? A letter commenting on Lytvyn et al [PDF]

Infection Control & Hospital Epidemiology, 2017
Stone, SP, Wilcox, M, Hawkey, P
openaire   +3 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

A Systematic Critical Appraisal of Clinical Practice Guidelines for Non-Pharmacological ‎Conservative Management of Complex Regional Pain Syndrome (CRPS) Using the ‎AGREE-II Instrument. [PDF]

J Eval Clin Pract
Shafiee E, MacDermid J, Packham T, Grewal R, Walton D, Farzad M.   +5 more
europepmc   +1 more source