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An Optimized Editing Approach for Wheat Genes by Improving sgRNA Design and Transformation Strategies. [PDF]
Int J Mol SciZhang RX +9 more
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Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
The Plant Journal, 1998The Agrobacterium vacuum infiltration method has made it possible to transform Arabidopsis thaliana without plant tissue culture or regeneration. In the present study, this method was evaluated and a substantially modified transformation method was ...
S. Clough, A. Bent
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1998
Virulent strains of Agrobacterium tumefaciens and Agrobacterium rhizogenes induce non-self limiting — neoplastic — growths on susceptible plants, generally in dicotyledonous species from the Angiosperms. In most cases, A. tumefaciens induces unorganized “crown gall” tumors (so named because the growths were often observed at the crown of the plant ...
BINNS A. N., COSTANTINO, Paolo
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Virulent strains of Agrobacterium tumefaciens and Agrobacterium rhizogenes induce non-self limiting — neoplastic — growths on susceptible plants, generally in dicotyledonous species from the Angiosperms. In most cases, A. tumefaciens induces unorganized “crown gall” tumors (so named because the growths were often observed at the crown of the plant ...
BINNS A. N., COSTANTINO, Paolo
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Opine biosynthesis and catabolism genes of Agrobacterium tumefaciens and Agrobacterium rhizogenes
Russian Journal of Genetics, 2015Agrobacterium is a genus of soil bacteria with the ability to transform plant cells by a T-DNA-sequence located on the pTi/pRi- plasmid containing a set of genes expressed in plant cells. Expression of these genes leads to a proliferation of transformed cells, with the subsequent formation of tumors or growths of roots and the synthesis of opines ...
I. Vladimirov, T. Matveeva, L. Lutova
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The Oncogenes of Agrobacterium Tumefaciens and Agrobacterium Rhizogenes
2008The common soil bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes are unique genetic pathogens capable of fundamentally redirecting plant metabolism in order to generate macroscopic tissue masses (crown galls and hairy roots, respectively) which support the growth of large populations of Agrobacteria.
Matthew A. Escobar +2 more
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Integration of Agrobacterium T-DNA into the Plant Genome.
Annual Review of Genetics, 2017Agrobacterium strains transfer a single-strand form of T-DNA (T-strands) and Virulence (Vir) effector proteins to plant cells. Following transfer, T-strands likely form complexes with Vir and plant proteins that traffic through the cytoplasm and enter ...
S. Gelvin
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Electroporation Protocols for Agrobacterium
2003Agrobacterium spp. are widely used to transform new genes into plant tissue. Transferring useful genes into bacteria in order to transform them into the target plant is an essential part of the process, which can be achieved by electroporation. Electroporation is a technique involving exposing cells to an electric field for a short duration, in order ...
Main, Garry D. +2 more
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The T-pilus of Agrobacterium tumefaciens
Trends in Microbiology, 2000T-pilus biogenesis uses a conserved transmembrane nucleoprotein- and protein-transport apparatus for the transport of cyclic T-pilin subunits to the Agrobacterium cell surface. T-pilin subunits are processed from full-length VirB2 pro-pilin into a cyclized peptide, a rapid reaction that is Agrobacterium specific and can occur in the absence of Ti ...
Clarence I. Kado, Erh-Min Lai
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1981
The genesis of the genus Agrobacterium, a member of the family Rhizobiaceae (Buchanan and Gibbons, 1974), can be traced to the discovery by Smith and Townsend (1907) of the causal agent of crown gall diseases of plants (now called Agrobacterium tumefaciens; Allen and Holding, 1974) and, some years earlier, by Beijerinck and van Delden (1902) of the ...
James A. Lippincott +2 more
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The genesis of the genus Agrobacterium, a member of the family Rhizobiaceae (Buchanan and Gibbons, 1974), can be traced to the discovery by Smith and Townsend (1907) of the causal agent of crown gall diseases of plants (now called Agrobacterium tumefaciens; Allen and Holding, 1974) and, some years earlier, by Beijerinck and van Delden (1902) of the ...
James A. Lippincott +2 more
openaire +2 more sources