A highly efficient genetic transformation system for broccoli and subcellular localization
IntroductionAgrobacterium-mediated genetic transformation has been widely used for the identification of functional genes and regulatory and developmental mechanisms in plants.
Yongyu Zhao +4 more
doaj +1 more source
A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing. [PDF]
Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), and Argonaute proteins (AGOs).
Jiang, Cai-Zhong +5 more
core +1 more source
Marker-free transgenic cucumber expressing Arabidopsis cbf1 gene confers chilling stress tolerance
Marker-free transgenic cucumber (Cucumis sativus L.) cv. Poinsett 76 SR plants were produced by Agrobacterium mediated transformation. A transformation efficiency of 1.62 was observed on using Agrobacterium tumefaciens strain LBA4404 harbouring ...
N. Gupta +6 more
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Transformation And Light Inducible Expression of cry1Ab Gene in Oilseed Rape (Brassica napus L.) [PDF]
Rapeseed (Brassica napus L.) is the third most important oil crop in global productions. One of the major limiting factors for oilseed rape production is lepidopteran pests of the Brassicaceae family.
H. Rahnama, M. Sheykhhasan
doaj
Approved genetically modified (GM) horticultural plants: A 25-year perspective
In this review, we present genetically modified (GM) horticultural events that have passed the regulatory process and have been approved for cultivation or food use in different countries.
Baranski Rafal +2 more
doaj +1 more source
Problems and possibilities of monocot transformation
Biotechnological improvement of monocots is often hampered by the lack of efficient regeneration systems, requisite wound responses and low cell competence. Despite these limitations, the biolistic and Agrobacterium methods have been successfully used to
P. Sood, A. Bhattacharya, A. Sood
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This little memoir is not a review; the reader is directed to current authoritative Agrobacterium reviews with genetic (23) or cell biology emphasis (24). Likewise, this is not an update on recent advances in plant genetic engineering, which are the subject of a recent book (13).
openaire +2 more sources
A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants [PDF]
Transposition of the Anthirrinum majus Tam3 element and the Zea mays Ac element has been monitored in petunia and tobacco plants. Plant vectors were constructed with the transposable elements cloned into the leader sequence of a marker gene ...
A Day +41 more
core +7 more sources
A novel expression cassette for the efficient visual selection of transformed tissues in florists' chrysanthemum (Chrysanthemum morifolium Ramat.). [PDF]
Constructs carrying visual reporter genes coupled with efficient promoters could facilitate the process of identification and selection of stable transformants in recalcitrant crops.
Jongsma, M.A. +3 more
core +2 more sources
A universal primer for isolation of fragments of a gene encoding phytoene desaturase for use in virus-induced gene silencing (VIGS) studies [PDF]
We have been using Virus-Induced Gene Silencing (VIGS) to test the function of genes that are candidates for involvement in floral senescence. Although VIGS is a powerful tool for assaying the effects of gene silencing in plants, relatively few taxa have
Cai-Zhong, J. +4 more
core +1 more source

