Results 31 to 40 of about 166,091 (383)
Application of recombinant DNA technology to studies on plant secondary metabolism [PDF]
, 2001 This review which is concerned with the application of recombinant DNA technology to studies on plant secondary metabolism, presents the more common plant transformation strategies and shows how these genetic approaches are being used in attempts to ...
Yeoman, Michael M., Zárate, Rafael
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Efficient CRISPR-mediated base editing in Agrobacterium spp.
Proceedings of the National Academy of Sciences of the United States of America, 2020 Significance Agrobacteria are plant-pathogenic bacteria that can deliver DNA to plant cells as part of their infection strategy. This property has been used for decades to generate transgenic plants and, more recently, to deliver gene-editing reagents to
S. Rodrigues +13 more
semanticscholar +1 more source
Effect of P19 gene expression on transcription rate and production of recombinant human tissue plasminogen (rtPA) in tobacco (nicotiana benthamiana) [PDF]
مجله بیوتکنولوژی کشاورزی, 2020 Objective Tissue plasminogen activator is one of the most important drugs in the treatment of heart disease. This drug is produced in the expression system as a recombinant protein that has high production costs.
Yousef Sharafi +2 more
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All Life, 2022 Submergence stress in plants subjected to flooding is a significant issue in agriculture. Decades of previous research have elucidated many molecular and physiological responses of plants to this stress. Since Arabidopsis thaliana has been established as
Mayra Santiago-Velasco +5 more
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Transformation of lily by Agrobacterium
Euphytica, 1995 Lily cv. Harmony was inoculated with several Agrobacterium strains to study its susceptibility to Agrobacterium infection and transformation. Tumorous tissue formation on inoculated stem internodes of sterile-grown plantlets, as well as expression of a β-glucuronidase marker gene interrupted by an intron in cells of inoculated stem nodes, indicate that
Langeveld, Simon A. +4 more
openaire +2 more sources
The significance of C-terminal NLS sequences of VirD2 in its nuclear localization in Saccharomyces cerevisiae [PDF]
Journal of Cell and Molecular Research, 2013 Agrobacterium tumefaciens is capable of gene transfer to both plant and non-plant organisms. Indeed, upon infection of eukaryotic cells, Agrobacterium tumefaciens transfers a piece of its tumor inducing (Ti)-plasmid, called T-DNA, to the host cell ...
Jalal Soltani +3 more
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Journal of Experimental Botany, 2019 A comparison of different CRISPR systems and promoters using Agrobacterium-mediated transformation in wheat shows the SpCas9 system to be the most efficient for genome editing, and a high haploid induction rate is achieved by editing TaMTL.
Huiyun Liu +7 more
semanticscholar +1 more source
International Journal of Secondary Metabolite, 2018 The procedure for vacuum infiltration of cultivars A. caudatus L. and hybrids of A. caudatus L. xA. paniculatus L. was optimized. The functioning of gene construction pCBv19 in the Amaranthus leaves was evaluated by the transient expression after vacuum ...
Olha Mikolaivna Yaroshko +1 more
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From A. rhizogenes RolD to Plant P5CS: Exploiting Proline to Control Plant Development [PDF]
, 2018 The capability of the soil bacterium Agrobacterium rhizogenes to reprogram plant development and induce adventitious hairy roots relies on the expression of a few root-inducing genes (rol A, B, C and D), which can be transferred from large virulence ...
Costantino, Paolo +2 more
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Development of an Agrobacterium‐delivered CRISPR/Cas9 system for wheat genome editing
Plant Biotechnology Journal, 2019 Summary CRISPR/Cas9 has been widely used for genome editing in many organisms, including important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the application of this powerful genome editing tool also depends on DNA ...
Zhengzhi Zhang +6 more
semanticscholar +1 more source