A highly efficient genetic transformation system for broccoli and subcellular localization
IntroductionAgrobacterium-mediated genetic transformation has been widely used for the identification of functional genes and regulatory and developmental mechanisms in plants.
Yongyu Zhao +4 more
doaj +1 more source
A novel expression cassette for the efficient visual selection of transformed tissues in florists' chrysanthemum (Chrysanthemum morifolium Ramat.). [PDF]
Constructs carrying visual reporter genes coupled with efficient promoters could facilitate the process of identification and selection of stable transformants in recalcitrant crops.
Jongsma, M.A. +3 more
core +2 more sources
Marker-free transgenic cucumber expressing Arabidopsis cbf1 gene confers chilling stress tolerance
Marker-free transgenic cucumber (Cucumis sativus L.) cv. Poinsett 76 SR plants were produced by Agrobacterium mediated transformation. A transformation efficiency of 1.62 was observed on using Agrobacterium tumefaciens strain LBA4404 harbouring ...
N. Gupta +6 more
doaj +1 more source
Transformation And Light Inducible Expression of cry1Ab Gene in Oilseed Rape (Brassica napus L.) [PDF]
Rapeseed (Brassica napus L.) is the third most important oil crop in global productions. One of the major limiting factors for oilseed rape production is lepidopteran pests of the Brassicaceae family.
H. Rahnama, M. Sheykhhasan
doaj
Approved genetically modified (GM) horticultural plants: A 25-year perspective
In this review, we present genetically modified (GM) horticultural events that have passed the regulatory process and have been approved for cultivation or food use in different countries.
Baranski Rafal +2 more
doaj +1 more source
Problems and possibilities of monocot transformation
Biotechnological improvement of monocots is often hampered by the lack of efficient regeneration systems, requisite wound responses and low cell competence. Despite these limitations, the biolistic and Agrobacterium methods have been successfully used to
P. Sood, A. Bhattacharya, A. Sood
doaj +1 more source
Application of recombinant DNA technology to studies on plant secondary metabolism [PDF]
This review which is concerned with the application of recombinant DNA technology to studies on plant secondary metabolism, presents the more common plant transformation strategies and shows how these genetic approaches are being used in attempts to ...
Yeoman, Michael M., Zárate, Rafael
core +1 more source
This little memoir is not a review; the reader is directed to current authoritative Agrobacterium reviews with genetic (23) or cell biology emphasis (24). Likewise, this is not an update on recent advances in plant genetic engineering, which are the subject of a recent book (13).
openaire +2 more sources
A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing. [PDF]
Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), and Argonaute proteins (AGOs).
Jiang, Cai-Zhong +5 more
core +1 more source
Agrobacterium: nature’s genetic engineer [PDF]
Agrobacterium was identified as the agent causing the plant tumor, crown gall over 100 years ago. Since then, studies have resulted in many surprising observations. Armin Braun demonstrated that Agrobacterium infected cells had unusual nutritional properties, and that the bacterium was necessary to start the infection but not for continued tumor ...
openaire +3 more sources

