Manipulation of Cannabinoid Biosynthesis via Transient RNAi Expression
Cannabis sativa L. produces unique phytocannabinoids, which are used for their pharmaceutical benefits. To date, there are no reports of in vivo engineering targeting the cannabinoid biosynthesis genes to greater elucidate the role each of these genes ...
Lennon Matchett-Oates+5 more
doaj +1 more source
Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other -Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat [PDF]
Root mat of cucumbers and tomatoes has previously been shown to be caused by Agrobacterium radiobacter strains harboring a root-inducing Ri plasmid (pRi).
Stead, D.E., Weller, S.A., Young, J.P.W.
core +1 more source
The RNA N6‐methyladenosine demethylase ALKBH9B modulates ABA responses in Arabidopsis
The RNA m6A demethylase ALKBH9B removes m6A from the ABSCISIC ACID (ABA) INSENSITIVE 1 (ABI1) and BRI1‐EMS‐SUPPRESSOR 1 (BES1) transcripts, thus stabilizing them and leading to the inhibition of ABI3 and ABI5 by ABI1 and BES1 proteins, promoting seed germination and early seedling development under ABA treatment.
Jun Tang+5 more
wiley +1 more source
Plant Cellular and Molecular Biotechnology: Following Mariotti's Steps [PDF]
This review is dedicated to the memory of Prof. Domenico Mariotti, who significantly contributed to establishing the Italian research community in Agricultural Genetics and carried out the first experiments of Agrobacterium-mediated plant genetic ...
Caretto, Sofia+11 more
core +1 more source
Genome editing tools have rapidly been adopted by plant scientists for gene function discovery and crop improvement. The current technical challenge is to efficiently induce precise and predictable targeted point mutations valuable for crop breeding ...
F. Veillet+7 more
semanticscholar +1 more source
Efficient CRISPR-mediated base editing in Agrobacterium spp.
Significance Agrobacteria are plant-pathogenic bacteria that can deliver DNA to plant cells as part of their infection strategy. This property has been used for decades to generate transgenic plants and, more recently, to deliver gene-editing reagents to
S. Rodrigues+13 more
semanticscholar +1 more source
Cyclic β-glucans at the bacteria–host cells interphase: One sugar ring to rule them all [PDF]
Cyclic β‐1,2‐D‐glucans (CβG) are natural bionanopolymers present in the periplasmic space of many Proteobacteria. These molecules are sugar rings made of 17 to 25 D‐glucose units linked exclusively by β‐1,2‐glycosidic bonds.
Arce Gorvel, Vilma+4 more
core +1 more source
Binary Agrobacterium vectors for plant transformation.
A vector molecule for the efficient transformation of higher plants has been constructed with several features that make it efficient to use. It utilizes the trans acting functions of the vir region of a co-resident Ti plasmid in Agrobacterium ...
M. Bevan
semanticscholar +1 more source
Agrobacterium-mediated transformation of wheat (Triticum aestivum) using chitinase and glucanase genes [PDF]
Production of transgenic wheat (Triticum Aestivum) was studied by chitinase and glucanase genes by Agrobacterium-mediated transformation and also pBI121 plasmid containing neomycin phospho transferasa selectable marker gene under control of Nos promoter.
Negin Mohammadi zadeh+2 more
doaj +1 more source
Development of an Agrobacterium‐delivered CRISPR/Cas9 system for wheat genome editing
Summary CRISPR/Cas9 has been widely used for genome editing in many organisms, including important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the application of this powerful genome editing tool also depends on DNA ...
Zhengzhi Zhang+6 more
semanticscholar +1 more source