Pea transformation: History, current status and challenges
Czech Journal of Genetics and Plant Breeding, 2022 This review recapitulates the history, important milestones, the current status, and the perspectives of the pea (Pisum sativum L.) transformation as a tool for pea crop breeding.
Michaela Ludvíková, Miroslav Griga
doaj +1 more source
Establishment of Hevea brasiliensis lines overexpressing genes involved in ethylene signalling pathway [PDF]
, 2016 The gaseous plant hormone ethylene has a wide variety of applications in agriculture and horticulture. Ethylene Response Factors (ERF) are the last transcription factors of the ethylene signalling pathway and control a large number of ethylene-responsive
Dessailly, Florence +6 more
core +2 more sources
An Agrobacterium‐delivered CRISPR/Cas9 system for high‐frequency targeted mutagenesis in maize
Plant Biotechnology Journal, 2016 Summary CRISPR/Cas9 is a powerful genome editing tool in many organisms, including a number of monocots and dicots. Although the design and application of CRISPR/Cas9 is simpler compared to other nuclease‐based genome editing tools, optimization requires
S. N. Char +10 more
semanticscholar +1 more source
Genetic Manipulation and Transformation Methods for Aspergillus spp.
Mycobiology, 2021 Species of the genus Aspergillus have a variety of effects on humans and have been considered industrial cell factories due to their prominent ability for manufacturing several products such as heterologous proteins, secondary metabolites, and organic ...
Ye-Eun Son, Hee-Soo Park
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A universal primer for isolation of fragments of a gene encoding phytoene desaturase for use in virus-induced gene silencing (VIGS) studies [PDF]
, 2010 We have been using Virus-Induced Gene Silencing (VIGS) to test the function of genes that are candidates for involvement in floral senescence. Although VIGS is a powerful tool for assaying the effects of gene silencing in plants, relatively few taxa have
Cai-Zhong, J. +4 more
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Transfection in Agrobacterium tumefaciens [PDF]
Journal of Virology, 1972 Intact cells of Agrobacterium tumefaciens were examined for ability to take up biologically active LR-4 phage deoxyribonucleic acid (DNA) from the surrounding medium. DNA incorporation as measured by subsequent plaque formation (transfection) failed to occur when the bacteria were grown in defined minimal salts ...
Victor J. Milani, Gary T. Heberlein
openaire +3 more sources
Assessment of RNAi-induced silencing in banana (Musa spp.) [PDF]
, 2015 In plants, RNA- based gene silencing mediated by small RNAs functions at the transcriptional or post-transcriptional level to negatively regulate target genes, repetitive sequences, viral RNAs and/or transposon elements.
Cammue, B.P.A. +6 more
core +1 more source
Agrobacterium-Mediated Plant Transformation: Biology and Applications
The Arabidopsis Book, 2017 Plant genetic transformation heavily relies on the bacterial pathogen Agrobacterium tumefaciens as a powerful tool to deliver genes of interest into a host plant.
Hau-Hsuan Hwang, Manda Yu, E. Lai
semanticscholar +1 more source
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
FEBS Letters, EarlyView.This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Optimization of Agrobacterium-Mediated Transformation in Soybean
Frontiers in Plant Science, 2017 High transformation efficiency is a prerequisite for study of gene function and molecular breeding. Agrobacterium tumefaciens-mediated transformation is a preferred method in many plants.
Shuxuan Li +7 more
semanticscholar +1 more source