Results 51 to 60 of about 75,766 (335)
Differentiation of Phytopathogenic Agrobacterium spp. [PDF]
Due to the difficulties in differentiation of phytopathogenic Agrobacterium spp. and lack of a standardized protocol, we carried out selection and evaluation of suitable methods based on the bacterial physiological, genetic and pathogenic properties ...
Nemanja Kuzmanović+4 more
doaj
Progress of cereal transformation technology mediated by Agrobacterium tumefaciens
Monocotyledonous plants were believed to be not transformable by the soil bacterium Agrobacterium tumefaciens until two decades ago, although convenient protocols for infection of leaf disks and subsequent regeneration of transgenic plants had been well ...
Yukoh Hiei, Y. Ishida, T. Komari
semanticscholar +1 more source
AlphaFold‐Guided Bespoke Gene Editing Enhances Field‐Grown Soybean Oil Contents
An AlphaFold‐guided method is developed to functionally optimize soybean sugar transporters and achieve bespoke gene editing of GmSWEET10a/b to improve oil content in an elite soybean cultivar in multi‐year, multi‐site field trials. The combination of AI‐guided protein design and gene editing may unlock a huge potential to improve the genetic trait de ...
Jie Wang+22 more
wiley +1 more source
Ferrisiderophore reductase activity in Agrobacterium tumefaciens [PDF]
Reduction of the iron in ferriagrobactin by the cytoplasmic fraction of Agrobacterium tumefaciens strictly required NaDH as the reductant. Addition of flavin mononucleotide and anaerobic conditions were necessary for the reaction; when added with flavin mononucleotide, magnesium was stimulatory. This ferrisiderophore reductase activity may be a part of
Jean E. L. Arceneaux+3 more
openaire +3 more sources
The synthesis of peptidoglycan (PG) in bacteria is a crucial process controlling cell shape and vitality. In contrast to bacteria such as Escherichia coli that grow by dispersed lateral insertion of PG, little is known of the processes that direct polar ...
Todd A. Cameron+4 more
semanticscholar +1 more source
In early cold response, MdICE1L utilizes MdHB7L as a cofactor to facilitate the transcriptional activation of MdCBFs, thereby activating cold signaling rapidly and strongly. Subsequently, MdICE1L is degraded by MdHOS1. Meanwhile, MdHB7L is released and accumulates.
Jie Yang+8 more
wiley +1 more source
Agrobacterium tumefaciens isolated from hemodialysis water [PDF]
nema
Smieško Gordana, Gusman Vera
doaj +1 more source
The Agrobacterium-mediated transient gene expression system is a rapid and efficient method for heterologous recombinant protein expression in plants. The fermentation of genetically modified Agrobacterium tumefaciens is an important step in increasing ...
Nisit Watthanasakphuban+6 more
doaj +1 more source
Chloroplast transformation by Agrobacterium tumefaciens [PDF]
A chimeric gene consisting of the promoter region of the nopaline synthase gene (Pnos) fused to the coding sequence of the chloramphenicol acetyltransferase gene (cat gene) of Tn9 was introduced by co-cultivation in tobacco protoplasts followed by selection with 10 mug/ml chloramphenicol. The chloramphenicol-resistant plants derived from these selected
Marc Van Montagu+2 more
openaire +3 more sources
Agrobacterium tumefaciens responses to plant-derived signaling molecules
As a special phytopathogen, Agrobacterium tumefaciens infects a wide range of plant hosts and causes plant tumors also known as crown galls. The complexity of Agrobacterium–plant interaction has been studied for several decades.
S. Subramoni+3 more
semanticscholar +1 more source