Results 61 to 70 of about 1,123,733 (296)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Air quality assessment for Portugal [PDF]
, 2007 According to the Air Quality Framework Directive, air pollutant concentration levels have to be assessed and reported annually by each European Union member state, taking into consideration European air quality standards.
A. Monteiro +24 more
core +3 more sources
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Noise Mapping, 2020 LIFE MONZA project (Methodologies fOr Noise low emission Zones introduction And management) aims at defining an easy-replicable method for the identification and management of the Noise Low Emission Zones (Noise LEZ), urban areas subject to traffic ...
Silvaggio Rosalba +18 more
doaj +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
A proof of concept on real-time air quality monitoring system [PDF]
, 2017 According to the Department of Environment Malaysia, Air Pollutant Index (API) is an indicator for the air quality status at any particular area. API is calculated based on average concentration of air pollutants such as Sulphur Dioxide (SO2), Nitrogen ...
Ibrahim, Noraini +2 more
core
FEBS Open Bio, EarlyView.Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero +5 more
wiley +1 more source
Exposure to hazardous volatile organic compounds, PM 10 and CO while walking along streets in urban Guangzhou, China [PDF]
, 2004 Toxic air pollutants in street canyons are important issues concerning public health especially in some large Asian cities like Guangzhou. In 1998 <18% of Guangzhou citizens used public transportation modes, with a majority commuting on foot (42%) or ...
Blake, DR +7 more
core
The state of ambient air quality in Pakistan—a review [PDF]
, 2010 Background and purpose: Pakistan, during the last decade, has seen an extensive escalation in population growth, urbanization, and industrialization, together with a great increase in motorization and energy use. As a result, a substantial rise has taken
A Aziz +74 more
core +1 more source