Results 21 to 30 of about 2,776 (192)

Reverse protein engineering of a novel 4‐domain copper nitrite reductase reveals functional regulation by protein–protein interaction

The FEBS Journal, Volume 288, Issue 1, Page 262-280, January 2021., 2021
We discovered a new class of 4‐domain Cu‐containing nitrite reductase (CuNiR) from Bradyrhizobium sp. ORS 375 that has both cytochrome (Cytc) and cupredoxin (Cup) domains tethered to catalytic core (Core) domain at the N‐terminus. This article reports the first characterization of this enzyme (BrNiR) and its domain‐truncated 3‐ and 2‐domain mutants ...
Daisuke Sasaki, Tatiana F. Watanabe, Robert R. Eady, Richard C. Garratt, Svetlana V. Antonyuk, S. Samar Hasnain   +5 more
wiley   +1 more source

rRNA Analysis Based on Long‐Read High‐Throughput Sequencing Reveals a More Accurate Diagnostic for the Bacterial Infection of Ascites

BioMed Research International, Volume 2021, Issue 1, 2021., 2021
Traditional pathogenic diagnosis presents defects such as a low positivity rate, inability to identify uncultured microorganisms, and time‐consuming nature. Clinical metagenomics next‐generation sequencing can be used to detect any pathogen, compensating for the shortcomings of traditional pathogenic diagnosis.
Xiaoling Yu, Wenqian Jiang, Xinhui Huang, Jun Lin, Hanhui Ye, Baorong Liu, Dorota Formanowicz   +6 more
wiley   +1 more source

Factors Influencing the Bacterial Bioremediation of Hydrocarbon Contaminants in the Soil: Mechanisms and Impacts

Journal of Chemistry, Volume 2021, Issue 1, 2021., 2021
The discharge of hydrocarbons and their derivatives to environments due to human and/or natural activities cause environmental pollution (soil, water, and air) and affect the natural functioning of an ecosystem. To minimize or eradicate environmental pollution by hydrocarbon contaminants, studies showed strategies including physical, chemical, and ...
Gessesse Kebede, Tekle Tafese, Ebrahim M. Abda, M. Kamaraj, Fassil Assefa, Yuhe He   +5 more
wiley   +1 more source

A 2.2 Å cryoEM structure of a quinol-dependent NO Reductase shows close similarity to respiratory oxidases

Nature Communications, 2023
Quinol-dependent nitric oxide reductases (qNORs) are considered members of the respiratory heme-copper oxidase superfamily, are unique to bacteria, and are commonly found in pathogenic bacteria where they play a role in combating the host immune response.
Alex J. Flynn, Svetlana V. Antonyuk, Robert R. Eady, Stephen P. Muench, S. Samar Hasnain   +4 more
doaj   +1 more source

Avaliação da contaminação bacteriana em desinfetantes de uso domiciliar Evaluation of bacterial contamination in disinfectants for domestic use

Revista de Saúde Pública, 2000
OBJETIVO: Avaliar desinfetantes de uso domiciliar, identificando a presença de bactérias contaminantes, e conhecer o nível de tolerância dessas bactérias ao cloreto de benzalcônio.
Fumie Miyagi, Jorge Timenetsky, Flávio Alterthum   +2 more
doaj   +3 more sources

Active Intermediates in Copper Nitrite Reductase Reactions Probed by a Cryotrapping‐Electron Paramagnetic Resonance Approach

Angewandte Chemie, Volume 132, Issue 33, Page 14040-14044, August 10, 2020., 2020
Metalloenzyme catalysis: Through the use of cryoreduction and annealing, a method of studying electron transfer in redox active metalloenzymes is developed. Combined with electron paramagnetic resonance spectroscopy, active states in the catalytic cycle of the copper containing nitrite reductases are probed.
Tobias M. Hedison, Muralidharan Shanmugam, Derren J. Heyes, Ruth Edge, Nigel S. Scrutton   +4 more
wiley   +2 more sources

Study of the Cys-His bridge electron transfer pathway in a copper-containing nitrite reductase by site-directed mutagenesis, spectroscopic, and computational methods [PDF]

, 2018
The Cys-His bridge as electron transfer conduit in the enzymatic catalysis of nitrite to nitric oxide by nitrite reductase from Sinorhizobium meliloti 2011 (SmNir) was evaluated by site-directed mutagenesis, steady state kinetic studies, UV-vis and EPR ...
Brondino, Carlos Dante, Cristaldi, Julio César, Dalosto, Sergio Daniel, Ferroni, Felix Martín, González, Pablo Javier, Gómez, María C., Rivas, Maria Gabriela, Rizzi, Alberto Claudio   +7 more
core   +1 more source

respiratory tract infection in cystic fibrosis patients [PDF]

, 2011
The aims of this study were to evaluate the frequency of Achromobacter xylosoxidans infection in a cohort of cystic fibrosis patients, to investigate antimicrobial sensitivity, to establish possible clonal likeness among strains, and to address the ...
A. Lambiase, M. R. Catania, M. del Pezzo, F. Rossano, V. Terlizzi, A. Sepe, V. Raia   +6 more
core   +1 more source

Complete Genome Sequence of the Cystic Fibrosis Pathogen Achromobacter xylosoxidans NH44784-1996 Complies with Important Pathogenic Phenotypes [PDF]

, 2013
Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A.
Alhede, M, Bjarnsholt, T, Burmølle, M, Ciofu, O, Cockburn, A, Eickhardt-Sørensen, S, Givskov, M, Hansen, L, Hansen, M, Hansen, MA, Hoiby, N, Jakobsen, TH, Jensen, PØ, Kerpedjiev, P, Kolpen, M, Kuhl, M, Moser, C, Qvortrup, K, Riber, L, Ridderberg, W, Rønne Hansen, C, Sørensen, S   +21 more
core   +4 more sources

Ribosomal DNA-Directed PCR for Identification of Achromobacter (Alcaligenes) xylosoxidans Recovered from Sputum Samples from Cystic Fibrosis Patients [PDF]

Journal of Clinical Microbiology, 2002
Tom Coenye, John J LiPuma
exaly   +2 more sources