Results 211 to 220 of about 8,032 (242)
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[110] Bacterial alginate lyase
1966Publisher Summary This chapter discusses the synthesis of bacterial alginate lyase. The enzyme occurs in an organism isolated from Potomac Bay mud capable of utilizing alginic acid as a sole carbon source. The organism was classified as a pseudomonad 1 and can be obtained from the American Type Culture Collection.
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World Journal of Microbiology and Biotechnology, 2007
The alyPEEC gene encoding alginate lyase from marine bacterium Pseudoalteromonas elyakovii IAM 14594 was subcloned into pBAD24 with arabinose promoter and sequenced, and overexpressed in TOP10 strain of E. coli after arabinose induction. Expression levels of alyPEEC gene in E. coli cells were over 39.6-fold higher than those in P.
L. Y. Ma, Z. M. Chi, J. Li, L. F. Wu
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The alyPEEC gene encoding alginate lyase from marine bacterium Pseudoalteromonas elyakovii IAM 14594 was subcloned into pBAD24 with arabinose promoter and sequenced, and overexpressed in TOP10 strain of E. coli after arabinose induction. Expression levels of alyPEEC gene in E. coli cells were over 39.6-fold higher than those in P.
L. Y. Ma, Z. M. Chi, J. Li, L. F. Wu
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Enzymatic Properties of Alginate Lyase from Paenibacillus sp. S29
Applied Biochemistry and Biotechnology, 2017Paenibacillus sp. S29 was isolated from soil and produces an alginate lyase. The molecular weight of this enzyme was 32 kDa and the N-terminal amino acid sequence was ASVTKST. The optimal pH was approximately 8.7 and the enzyme was stable over a pH range of 5.6 to 8.8 at 40 °C for 60 min.
Masahiro, Kurakake +3 more
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Journal of Bioscience and Bioengineering, 2005
Sphingomonas sp. A1 (strain A1) produces three endotypes (A1-I [65 kDa], A1-II [25 kDa], and A1-III [40 kDa]) and an exotype (A1-IV [86 kDa]) alginate lyases in cytoplasm. These four enzymes cooperatively depolymerize alginate into constituent monosaccharides.
Wataru, Hashimoto +3 more
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Sphingomonas sp. A1 (strain A1) produces three endotypes (A1-I [65 kDa], A1-II [25 kDa], and A1-III [40 kDa]) and an exotype (A1-IV [86 kDa]) alginate lyases in cytoplasm. These four enzymes cooperatively depolymerize alginate into constituent monosaccharides.
Wataru, Hashimoto +3 more
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Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2019
With the discovery of the significant medicinal value of alginate oligosaccharides and bioethanol produced by microalgae, alginate lyase has been the focus of research in all fields. Five alginate lyase genes in cluster from Vibrio alginolyticus were cloned and expressed in Escherichia coli.
Jie, Gao +6 more
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With the discovery of the significant medicinal value of alginate oligosaccharides and bioethanol produced by microalgae, alginate lyase has been the focus of research in all fields. Five alginate lyase genes in cluster from Vibrio alginolyticus were cloned and expressed in Escherichia coli.
Jie, Gao +6 more
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Isolation of an alginate-degrading organism and purification of its alginate lyase
Journal of Fermentation and Bioengineering, 1991Abstract We isolated a bacterial strain, OS-ALG-9, which solubilized immobilized gel with calcium alginate in a reactor. It was identified as Pseudomonas sp. We studied the cultural conditions by using a 2.5- l jar fermentor and found that the growth and alginate-degrading enzyme production were optimal at pH 7.0 and 30°C.
Shinichi Kinoshita +4 more
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Protein Expression and Purification, 2003
Sphingomonas sp. A1 (strain A1) cells contain three kinds of endotype alginate lyases [A1-I, A1-II, and A1-III], all of which are formed from a common precursor through posttranslational processing. In addition to these lyases, another type of lyase (A1-IV) that acts on oligoalginates exists in the bacterium. A1-IV was overexpressed in Escherichia coli
Osamu, Miyake +2 more
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Sphingomonas sp. A1 (strain A1) cells contain three kinds of endotype alginate lyases [A1-I, A1-II, and A1-III], all of which are formed from a common precursor through posttranslational processing. In addition to these lyases, another type of lyase (A1-IV) that acts on oligoalginates exists in the bacterium. A1-IV was overexpressed in Escherichia coli
Osamu, Miyake +2 more
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Alginate Lyases of Pseudomonads*
The Journal of Biochemistry, 1969Y, Kashiwabara, H, Suzuki, K, Nisizawa
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[Advances in algae tool enzymes: alginate lyases].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2013Marine can be considered as a rather unexplored source of biological material. Production of algal oligosaccharides by using valuable enzymes from marine origin has become an important way to utilize marine resources. As one of algal tool enzymes, the use of alginate lyases has been focused mainly on development and application of alginate ...
Liyan, Li +3 more
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An electrically conductive silver–polyacrylamide–alginate hydrogel composite for soft electronics
Nature Electronics, 2021Yunsik Ohm +2 more
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