Results 211 to 220 of about 113,406 (275)

Action Pattern of a Novel G-Specific Alginate Lyase: Determination of Subsite Specificity by HPAEC-PAD/MS.

Journal of Agricultural and Food Chemistry, 2023
G-specific alginate lyases are important tools for alginate fragment biodegradation and oligosaccharide production, which have great potential in alginate refining research. In this research, a novel G-specific alginate lyase Aly7Ce was cloned, expressed,
Jiajing Li, Changhu Xue, Jingjing Shen, Guanchen Liu, Xuanwei Mei, Menghui Sun, Yaoguang Chang   +6 more
semanticscholar   +1 more source

A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates.

Journal of Agricultural and Food Chemistry
Alginate lyase Aly448, a potential new member of the polysaccharide lyase (PL) 7 family, which was cloned and identified from the macroalgae-associated bacterial metagenomic library, showed bifunctionality.
xiaoqian gu, Li-Zhong Fu, Zhiyan Wang, Zhe Cao, Luying Zhao, Dewi Seswita-Zilda, Ao Zhang, Qian Zhang, Jiang Li   +8 more
semanticscholar   +1 more source

Alginate production by an Azotobacter vinelandii mutant unable to produce alginate lyase

Applied Microbiology and Biotechnology, 2003
Alginate is an industrially relevant linear copolymer composed of beta-1,4-linked D-mannuronic acid and its C-5 epimer L-guluronic acid. The rheological and gel-forming properties of alginates depend on the molecular weight and the relative content of the two monomers. Alginate produced by Azotobacter vinelandii was shown to be degraded towards the end
M A, Trujillo-Roldán, S, Moreno, D, Segura, E, Galindo, G, Espín   +4 more
openaire   +2 more sources

Characterization of Alginate Lyase Activity on Liquid, Gelled, and Complexed States of Alginate

Biotechnology Progress, 2007
A study of alginate lyase was carried out to determine if this enzyme could be used to remove alginate present in the core of alginate/poly-L-lysine (AG/PLL) microcapsules in order to maximize cell growth and colonization. A complete kinetic study was undertaken, which indicated an optimal activity of the enzyme at pH 7-8, 50 degrees C, in the presence
Véronique, Breguet, Urs, von Stockar, Ian W, Marison   +2 more
openaire   +2 more sources

Efficient Production of an Alginate Lyase in Bacillus subtilis with Combined Strategy: Vector and Host Selection, Promoter and Signal Peptide Screening, and Modification of a Translation Initiation Region.

Journal of Agricultural and Food Chemistry
Alginate lyases (ALys) whose degrading products, alginate oligosaccharides, exhibit various outstanding biochemical activities have aroused increasing interest of researchers in the marine bioresource field.
Licheng Zhou, Ran Zhang, Bo Jiang, Qing Meng, Jingjing Chen, Xiaoyong Liu   +5 more
semanticscholar   +1 more source

Overexpression of alginate lyase of Pseudoalteromonas elyakovii in Escherichia coli, purification, and characterization of the recombinant alginate lyase

World Journal of Microbiology and Biotechnology, 2007
The alyPEEC gene encoding alginate lyase from marine bacterium Pseudoalteromonas elyakovii IAM 14594 was subcloned into pBAD24 with arabinose promoter and sequenced, and overexpressed in TOP10 strain of E. coli after arabinose induction. Expression levels of alyPEEC gene in E. coli cells were over 39.6-fold higher than those in P.
L. Y. Ma, Z. M. Chi, J. Li, L. F. Wu
openaire   +1 more source

Bacterial alginate lyase inactive on alginate biosynthesized by Pseudomonas aeruginosa

Journal of Fermentation and Bioengineering, 1993
A bacterium (strain Al) isolated from a ditch produces three kinds of intracellular alginate lyases [Al-I (molecular weight: M.W. 60,000), Al-II-1 (M.W. 60,000) and Al-II-2]; the former two lyases have been purified and characterized (Yonemoto et al., J. Ferment. Bioeng., 72, 152–157, 1991).
Tomohiro Hisano, Hisako Yamaguchi, Yoshimasa Yonemoto, Kenji Sakaguchi, Tetsuo Yamashita, Shiro Abe, Akira Kimura, Kousaku Murata   +7 more
openaire   +1 more source

Elucidation of degradation pattern and immobilization of a novel alginate lyase for preparation of alginate oligosaccharides

International Journal of Biological Macromolecules, 2020
Alginate lyases are important enzymes to prepare alginate oligosaccharides for industrial applications and elucidating the degradation pattern of alginate lyases is essential for expanding their applications. Herein, we cloned and expressed AlyPL6, a novel member of polysaccharide lyase family-6 (PL6) with high activity from Pedobacter hainanensis NJ ...
Qian, Li, Fu, Hu, Mingyang, Wang, Benwei, Zhu, Fang, Ni, Zhong, Yao   +5 more
openaire   +2 more sources

Isolation of an alginate-degrading organism and purification of its alginate lyase

Journal of Fermentation and Bioengineering, 1991
Abstract We isolated a bacterial strain, OS-ALG-9, which solubilized immobilized gel with calcium alginate in a reactor. It was identified as Pseudomonas sp. We studied the cultural conditions by using a 2.5- l jar fermentor and found that the growth and alginate-degrading enzyme production were optimal at pH 7.0 and 30°C.
Shinichi Kinoshita, Yasushi Kumoi, Atsumi Ohshima, Toshiomi Yoshida, Naoya Kasai   +4 more
openaire   +1 more source

Alginate lyase: Structure, property, and application

Biotechnology and Bioprocess Engineering, 2011
Alginate is a linear polysaccharide in which β-D-mannuronate (M) and its epimer, α-L-guluronate (G), are covalently (1–4)-linked in different sequences. Alginate is mainly used as a food additive to modify food texture due to its high viscosity and gelling property.
Hee Sook Kim, Choul-Gyun Lee, Eun Yeol Lee   +2 more
openaire   +1 more source