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Biocatalysis and Biotransformation
Alginate lyase, which catalyzes the cleavage of alginate, has potential biocatalytic applications in agriculture, food, fodder, nutraceutical, pharmaceuticals, medical diagnostic and bioenergy industries.
B. Mohapatra
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Alginate lyase, which catalyzes the cleavage of alginate, has potential biocatalytic applications in agriculture, food, fodder, nutraceutical, pharmaceuticals, medical diagnostic and bioenergy industries.
B. Mohapatra
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[110] Bacterial alginate lyase
1966Publisher Summary This chapter discusses the synthesis of bacterial alginate lyase. The enzyme occurs in an organism isolated from Potomac Bay mud capable of utilizing alginic acid as a sole carbon source. The organism was classified as a pseudomonad 1 and can be obtained from the American Type Culture Collection.
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Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2019
With the discovery of the significant medicinal value of alginate oligosaccharides and bioethanol produced by microalgae, alginate lyase has been the focus of research in all fields. Five alginate lyase genes in cluster from Vibrio alginolyticus were cloned and expressed in Escherichia coli.
Jie, Gao +6 more
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With the discovery of the significant medicinal value of alginate oligosaccharides and bioethanol produced by microalgae, alginate lyase has been the focus of research in all fields. Five alginate lyase genes in cluster from Vibrio alginolyticus were cloned and expressed in Escherichia coli.
Jie, Gao +6 more
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Isolation and characterization of an alginate lyase from Klebsiella aerogenes
Archives of Microbiology, 1989The bacterium Klebsiella aerogenes (type 25) produced an inducible alginate lyase, whose major activity was located intracellularly during all growth phases. The enzyme was purified from the soluble fraction of sonicated cells by ammonium sulfate precipitation, anion- and cation-exchange chromatography and gel filtration.
B, Lange, J, Wingender, U K, Winkler
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Journal of Bioscience and Bioengineering, 2005
Sphingomonas sp. A1 (strain A1) produces three endotypes (A1-I [65 kDa], A1-II [25 kDa], and A1-III [40 kDa]) and an exotype (A1-IV [86 kDa]) alginate lyases in cytoplasm. These four enzymes cooperatively depolymerize alginate into constituent monosaccharides.
Wataru, Hashimoto +3 more
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Sphingomonas sp. A1 (strain A1) produces three endotypes (A1-I [65 kDa], A1-II [25 kDa], and A1-III [40 kDa]) and an exotype (A1-IV [86 kDa]) alginate lyases in cytoplasm. These four enzymes cooperatively depolymerize alginate into constituent monosaccharides.
Wataru, Hashimoto +3 more
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Food Chemistry
To achieve the cost-effective alginate oligosaccharides production, we have developed a novel rational design strategy that optimized capture behavior, orientation movement and hydrogen bond interaction of substrate in alginate lyase ALYI1. This approach led to S56D and G258Q advantageous variants balancing the trade-off challenge, particularly S56D ...
Jin-Ping, Huang +9 more
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To achieve the cost-effective alginate oligosaccharides production, we have developed a novel rational design strategy that optimized capture behavior, orientation movement and hydrogen bond interaction of substrate in alginate lyase ALYI1. This approach led to S56D and G258Q advantageous variants balancing the trade-off challenge, particularly S56D ...
Jin-Ping, Huang +9 more
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Alginate Lyases of Pseudomonads*
The Journal of Biochemistry, 1969Y, Kashiwabara, H, Suzuki, K, Nisizawa
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International Journal of Biological Macromolecules
Alginate is mainly a linear polysaccharide composed of randomly arranged β-D-mannuronic acid and α-L-guluronic acid linked by α, β-(1,4)-glycosidic bonds. Alginate lyases degrade alginate mainly adopting a β-elimination mechanism, breaking the glycosidic bonds between the monomers and forming a double bond between the C4 and C5 sugar rings to produce ...
Chen, Chen +7 more
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Alginate is mainly a linear polysaccharide composed of randomly arranged β-D-mannuronic acid and α-L-guluronic acid linked by α, β-(1,4)-glycosidic bonds. Alginate lyases degrade alginate mainly adopting a β-elimination mechanism, breaking the glycosidic bonds between the monomers and forming a double bond between the C4 and C5 sugar rings to produce ...
Chen, Chen +7 more
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An electrically conductive silver–polyacrylamide–alginate hydrogel composite for soft electronics
Nature Electronics, 2021Yunsik Ohm +2 more
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Natural Biopolymer-Based Biocompatible Conductors for Stretchable Bioelectronics
Chemical Reviews, 2021Chunya Wang +2 more
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