Results 221 to 230 of about 111,667 (274)

Efficient Production of an Alginate Lyase in Bacillus subtilis with Combined Strategy: Vector and Host Selection, Promoter and Signal Peptide Screening, and Modification of a Translation Initiation Region.

Journal of Agricultural and Food Chemistry
Alginate lyases (ALys) whose degrading products, alginate oligosaccharides, exhibit various outstanding biochemical activities have aroused increasing interest of researchers in the marine bioresource field.
Licheng Zhou, Ran Zhang, Bo Jiang, Qing Meng, Jingjing Chen, Xiaoyong Liu   +5 more
semanticscholar   +1 more source

Isolation of an alginate-degrading organism and purification of its alginate lyase

Journal of Fermentation and Bioengineering, 1991
Abstract We isolated a bacterial strain, OS-ALG-9, which solubilized immobilized gel with calcium alginate in a reactor. It was identified as Pseudomonas sp. We studied the cultural conditions by using a 2.5- l jar fermentor and found that the growth and alginate-degrading enzyme production were optimal at pH 7.0 and 30°C.
Naoya Kasai, Shinichi Kinoshita, Yasushi Kumoi, Atsumi Ohshima, Toshiomi Yoshida   +4 more
openaire   +2 more sources

Molecular identification of Sphingomonas sp. A1 Alginate lyase (A1-IV′) as a member of novel polysaccharide lyase family 15 and implications in alginate lyase evolution

Journal of Bioscience and Bioengineering, 2005
Sphingomonas sp. A1 (strain A1) produces three endotypes (A1-I [65 kDa], A1-II [25 kDa], and A1-III [40 kDa]) and an exotype (A1-IV [86 kDa]) alginate lyases in cytoplasm. These four enzymes cooperatively depolymerize alginate into constituent monosaccharides.
Kousaku Murata, Akihito Ochiai, Osamu Miyake, Wataru Hashimoto   +3 more
openaire   +3 more sources

Biocatalytic traits of a new nanobiocatalyst prepared via immobilization of alginate lyase from a novel Streptomyces sp. LB55 onto silicon dioxide nanoparticles

Biocatalysis and Biotransformation
Alginate lyase, which catalyzes the cleavage of alginate, has potential biocatalytic applications in agriculture, food, fodder, nutraceutical, pharmaceuticals, medical diagnostic and bioenergy industries.
B. Mohapatra
semanticscholar   +1 more source

[110] Bacterial alginate lyase

1966
Publisher Summary This chapter discusses the synthesis of bacterial alginate lyase. The enzyme occurs in an organism isolated from Potomac Bay mud capable of utilizing alginic acid as a sole carbon source. The organism was classified as a pseudomonad 1 and can be obtained from the American Type Culture Collection.
openaire   +2 more sources

Characterization of alginate lyases from a marine bacterium

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1990
Abstract 1. 1. Alginate lyases from a marine bacterium, Pseudomonas sp., were partially purified from the culture medium and from bacterial cells. 2. 2. The extracellular enzyme (mol. wt 32,000) was mostly specific for polyguluronan and the intracellular enzyme (mol. wt 94,000) was more preferential for polymannuronan than for polyguluronan.
Tsuyoshi Muramatsu, Takako Sogi
openaire   +2 more sources

Bacterial alginate lyase: Enzymology, genetics and application

Journal of Fermentation and Bioengineering, 1993
Abstract Alginate is a heteropolysaccharide comprised of mannuronate and guluronate. Three different types of alginate-degrading enzymes, alginate lyases A1-I, A1-II and A1-III, were produced by a bacterium isolated from a ditch. A1-I (63 kDa) was active on both brown seaweed (non-acetylated) and bacterial (acetylated) alginates, whereas A1-II (23 ...
Kenji Sakaguchi, Shiro Abe, Tetsuo Yamashita, Kousaku Murata, Yoshimasa Yonemoto, Akira Kimura, Tomoko Inose, Masahiro Takagi, Tadayuki Imanaka, Tomohiro Hisano   +9 more
openaire   +2 more sources

Antibacterial activity of lyase-depolymerized products of alginate

Journal of Applied Phycology, 2005
A series of mannuronic acid (M-block) and guluronic acid (G-block) fractions (M1–M5 and G1–G5) with different molecular weights were obtained by lyase depolymerization of alginate and evaluated for in vitro antibacterial activity against 19 bacterial strains. The antibacterial data revealed that both types of fractions generally showed activity against
Xiaoke Hu, Xiaolu Jiang, Yan Liu, Huey-Min Hwang, Jun Gong, Huashi Guan   +5 more
openaire   +2 more sources

Isolation and characterization of an alginate lyase from Klebsiella aerogenes

Archives of Microbiology, 1989
The bacterium Klebsiella aerogenes (type 25) produced an inducible alginate lyase, whose major activity was located intracellularly during all growth phases. The enzyme was purified from the soluble fraction of sonicated cells by ammonium sulfate precipitation, anion- and cation-exchange chromatography and gel filtration.
Jost Wingender, Ulrich Winkler, Bernd Lange   +2 more
openaire   +3 more sources

Alginate Lyases of Pseudomonads*

The Journal of Biochemistry, 1969
Hiroshi Suzuki, Yoshiko Kashiwabara, Kazutosi Nisizawa   +2 more
openaire   +3 more sources