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Alkaline Phosphatase

Cold Spring Harbor Protocols, 2020
Several types of alkaline phosphatases (or alkaline phosphomonoesterase) are commonly used in molecular cloning, including bacterial alkaline phosphatase (BAP) and calf intestinal alkaline phosphatase (CIP, CIAP, or CAP). Similar enzymes isolated from more esoteric cold-blooded organisms (e.g., SAP from shrimp) have become available in recent years and
Michael R, Green, Joseph, Sambrook
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Thyroiditis and Alkaline Phosphatase

Annals of Internal Medicine, 1978
Excerpt To the editor: Dalovisio, Blonde, Cortez, and Pankey (Ann Intern Med88:505-507, 1978) have reported elevated alkaline phosphatase levels in three patients with subacute thyroiditis.
R, Tur-Kaspa, Y, Naparstek
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Structure of alkaline phosphatases

Clinica Chimica Acta, 1990
The crystal structure of alkaline phosphatase (AP) from Escherichia coli, which is a prototype for mammalian APs, has been refined to a crystallographic R-factor of 0.184 at 2.0 A resolution. During the course of the refinement residues 380 to 410 were retraced and 190 to 200 were shifted by one residue, and substantial changes in the active site of ...
E E, Kim, H W, Wyckoff
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The alkaline phosphatase anti‐alkaline phosphatase technique in dermatopathology

Journal of Cutaneous Pathology, 1987
The APAAP technique is an unlabelled antibody bridge technique which can be used on either frozen or paraffinembedded sections. One applies first a monoclonal antibody, then a polyclonal bridge antibody, and finally a soluble complex of alkaline phosphatase and monoclonal mouse anti‐alkaline phosphatase. Subsequently, the enzyme label is developed with
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Alkaline phosphatase in craniopharyngiomas

The Journal of Pathology, 1971
AbstractTHE derivation of the epithelium from which craniopharyngiomas arise is in doubt. Willis (1953) and Russell and Rubinstein (1959) state that these tumours are not distinguishable from “epitheliomas” in which squamous and basal cells are both present.
W R, Timperley, P, Turner, S, Davies
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Alkaline phosphatase in the thymus

Cells Tissues Organs, 1976
(1) Fetal thymuses, organs from patients who died from diseases that are not clinically known to be associated with concomitant lymphoid tissue involvement, as well as thymuses from patients dying from diseases which affect the lymphatic complex of the body, one way or another, have been investigated for their alkaline phosphatase activity, using ...
M T, Rakhawy, A A, Tarkhan, A M, Zakaria
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Radiolysis of an Alkaline Phosphatase

Radiation Research, 1974
Nonexponential losses of activity in alkaline phosphatase were observed with four substrates after gamma -irradiation. Those losses were at the same rates for each of the combinations of radicals generated in water under O/sub 2/, N/sub 2/, and N/sub 2/O.
K R, Lynn, W J, Skinner
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The Pancreas and Alkaline Phosphatase

Nature, 1946
IN various accounts of histochemical studies on the presence of alkaline phosphatase (substrate: sodium-β-glycerophosphate, pH. 9) in homologous organs, correspondences (for example, in kidney and intestine) have usually been stressed rather than differences.
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Thiophosphorylation of Alkaline Phosphatase

Nature New Biology, 1973
ALKALINE phosphatase from Escherichia coli has been reported to be phosphorylated by a phosphate ester substrate1, a particular feature being that only one of two potentially active sites appeared to be phosphorylated at any one time. Binding studies with inorganic phosphate have shown there is one high affinity and a second low affinity site at pH 8 ...
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Leukocyte alkaline phosphatase

American Journal of Hematology, 1978
In summary, LAP is an intriguing enzyme and its control is related to pituitary-adrenal function. A review of the changes in LAP activity which occur in some physiological conditions and in disease states has been presented. The function of LAP, however, is unknown.
D B, Okun, K R, Tanaka
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