Microbial Biotechnology, Volume 18, Issue 12, December 2025.Employing directed evolution, we modified AlkS to detect isobutanol, significantly improving biosensor specificity. Subsequent optimisations through site‐directed mutagenesis and promoter engineering further enhanced the dynamic range to 5.56‐fold change, equivalent to a 114% increase.
Jiaming Chen +5 more
wiley +1 more source
DNA Checkpoint and Repair Factors Are Nuclear Sensors for Intracellular Organelle Stresses-Inflammations and Cancers Can Have High Genomic Risks. [PDF]
, 2018 Under inflammatory conditions, inflammatory cells release reactive oxygen species (ROS) and reactive nitrogen species (RNS) which cause DNA damage. If not appropriately repaired, DNA damage leads to gene mutations and genomic instability.
Chen, Hongping +21 more
core +2 more sources
Scientific ReportsIntegral proteins or enzymes are still challenging to purify into their native state because of their need for an amphipathic environment and cofactors.
Satyapriy Das, Sangeeta Negi
doaj +1 more source
Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir
Frontiers in Microbiology, 2017 The goal of this work was to study the overall genomic diversity of microorganisms of the Dagang high-temperature oilfield (PRC) and to characterize the metabolically active fraction of these populations.
Tamara N. Nazina +8 more
doaj +1 more source
Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina [PDF]
, 2014 Buffalo (Bubalus bubalis) populations are spread across northern Argentina, and they share their habitat with bovines. Both species are susceptible to brucellosis, and they are under a National Plan of Control and Eradication.
Jacobo, Roberto +4 more
core
Understanding plant-microbe interactions for phytoremediation of petroleum-polluted soil.
PLoS ONE, 2011 Plant-microbe interactions are considered to be important processes determining the efficiency of phytoremediation of petroleum pollution, however relatively little is known about how these interactions are influenced by petroleum pollution.
Ming Nie +9 more
doaj +1 more source
Degradation of long-chain n-alkanes in soil microcosms by two actinobacteria [PDF]
, 2012 The ability of two recently isolated actinobacteria, that degrade medium and long chain n-alkanes in laboratory water medium, was investigated in soil microcosms using different standard soils that were artificially contaminated with n-alkanes of ...
DE PASQUALE, Claudio +3 more
core +1 more source
Phylogenomic analysis of integral diiron membrane histidine motif-containing enzymes in ciliates provides insights into their function and evolutionary relationships [PDF]
, 2017 The Integral Membrane Histidine Motif-containing Enzymes (IMHME) are a class of binuclear non-heme iron proteins widely distributed among prokaryotes and eukaryotes.
Cid, Nicolás Gonzalo +5 more
core +1 more source
Identity and mechanisms of alkane-oxidizing metalloenzymes from deep-sea hydrothermal vents
Frontiers in Microbiology, 2013 Six aerobic alkanotrophs (organism that can metabolize alkanes as their sole carbon source) isolated from deep-sea hydrothermal vents were characterized using the radical clock substrate norcarane to determine the metalloenzyme and reaction mechanism ...
Rachel Narehood Austin +5 more
doaj +1 more source
Identification and use of an alkane transporter plug-in for application in biocatalysis and whole-cell biosensing of alkanes [PDF]
, 2014 Effective application of whole-cell devices in synthetic biology and biocatalysis will always require consideration of the uptake of molecules of interest into the cell.
Baganz, Frank +10 more
core +2 more sources