Results 1 to 10 of about 1,971,711 (246)

The endocannabinoid hydrolase FAAH is an allosteric enzyme. [PDF]

Sci Rep, 2020
AbstractFatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. Parallel orientation of FAAH monomers likely allows both subunits to simultaneously recruit and cleave substrates.
Dainese E, Oddi S, Simonetti M, Sabatucci A, Angelucci CB, Ballone A, Dufrusine B, Fezza F, De Fabritiis G, Maccarrone M.   +9 more
europepmc   +11 more sources

Patterns of Spatiotemporal Organization in an Allosteric Enzyme Model [PDF]

Proceedings of the National Academy of Sciences, 1973
The behavior of a model for an allosteric enzyme oscillator activated by the reaction product is analyzed in the presence of diffusion. When the concentrations of the chemicals are fixed at the boundaries, dynamic dissipative structures are shown to arise in the form of propagating concentration waves.
Albert Goldbeter
semanticscholar   +6 more sources

Molecular Synchronization Waves in Arrays of Allosterically Regulated Enzymes [PDF]

Phys. Rev. Lett. 99, 048301 (2007), 2007
Spatiotemporal pattern formation in a product-activated enzymic reaction at high enzyme concentrations is investigated. Stochastic simulations show that catalytic turnover cycles of individual enzymes can become coherent and that complex wave patterns of molecular synchronization can develop. The analysis based on the mean-field approximation indicates
Vanessa Casagrande, Yuichi Togashi, Alexander S. Mikhailov   +2 more
arxiv   +9 more sources

Allosteric Enzyme-Based Biosensors—Kinetic Behaviours of Immobilised L-Lysine-α-Oxidase from Trichoderma viride: pH Influence and Allosteric Properties [PDF]

Biosensors, 2020
The present study describes the kinetics of L-lysine-α-oxidase (LO) from Trichoderma viride immobilised by co-crosslinking onto the surface of a Pt electrode. The resulting amperometric biosensor was able to analyse L-lysine, thus permitting a simple but
Antonio Guerrieri, Rosanna Ciriello, Giuliana Bianco, Francesca De Gennaro, Silvio Frascaro   +4 more
doaj   +4 more sources

Uncoupling conformational states from activity in an allosteric enzyme. [PDF]

Nat Commun, 2017
ATP-phosphoribosyltransferase (ATP-PRT) is a hexameric enzyme in conformational equilibrium between an open and seemingly active state and a closed and presumably inhibited form. The structure-function relationship of allosteric regulation in this system
Pisco JP, de Chiara C, Pacholarz KJ, Garza-Garcia A, Ogrodowicz RW, Walker PA, Barran PE, Smerdon SJ, de Carvalho LPS.   +8 more
europepmc   +2 more sources

Recombinant L-asparaginase 1 from Saccharomyces cerevisiae: an allosteric enzyme with antineoplastic activity. [PDF]

Sci Rep, 2016
L-asparaginase (L-ASNase) (EC 3.5.1.1) is an important enzyme for the treatment of acute lymphoblastic leukaemia. Currently, the enzyme is obtained from bacteria, Escherichia coli and Erwinia chrysanthemi.
Costa IM, Schultz L, de Araujo Bianchi Pedra B, Leite MS, Farsky SH, de Oliveira MA, Pessoa A, Monteiro G.   +7 more
europepmc   +2 more sources

Statistical Mechanics of Allosteric Enzymes [PDF]

Journal Physical Chemistry B 120 (2016) 6021-6037, 2017
The concept of allostery in which macromolecules switch between two different conformations is a central theme in biological processes ranging from gene regulation to cell signaling to enzymology. Allosteric enzymes pervade metabolic processes, yet a simple and unified treatment of the effects of allostery in enzymes has been lacking.
Einav, Tal, Mazutis, Linas, Phillips, Rob   +2 more
arxiv   +8 more sources

THE BINDING OF NICOTINAMIDE-ADENINE DINUCLEOTIDE TO YEAST D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE: TEMPERATURE-JUMP RELAXATION STUDIES ON THE MECHANISM OF AN ALLOSTERIC ENZYME

Proceedings of the National Academy of Sciences of the United States of America, 1966
Enzymes exhibiting sigmoidal response of rate and binding to the concentration of substrates, activators, or inhibitors have been implied in a number of regulating mechanisms involving feedback control.'-3 It has become clear that these cooperative ...
Kasper Kirschner, Manfred Eigen, Robert Bittman, B. VOIGT   +3 more
openalex   +2 more sources

Structural Basis for Allosteric Regulation of the Monomeric Allosteric Enzyme Human Glucokinase [PDF]

Structure, 2004
Glucokinase is a monomeric enzyme that displays a low affinity for glucose and a sigmoidal saturation curve for its substrate, two properties that are important for its playing the role of a glucose sensor in pancreas and liver. The molecular basis for these two properties is not well understood.
Yasufumi Nagata, Jun-ichi Eiki, Teruyuki Nishimura, Kenji Kamata, Morihiro Mitsuya   +4 more
openaire   +4 more sources

DNA Detection and Signal Amplification via an Engineered Allosteric Enzyme [PDF]

Journal of the American Chemical Society, 2002
Rapid, sensitive, and sequence-specific DNA detection can be achieved in one step using an engineered intrasterically regulated enzyme. The semi-synthetic inhibitor-DNA-enzyme (IDE) construct (left) rests in the inactive state but upon exposure to a ...
Alan Saghatelian, Kevin M. Guckian, Desiree A. Thayer, M. Reza Ghadiri   +3 more
openalex   +2 more sources