Results 91 to 100 of about 2,040,864 (360)

High Resolution Crystal Structure of the Pyruvate Kinase Tetramer in Complex with the Allosteric Activator Mitapivat/AG-348

Crystals
Pyruvate kinase (PK) deficiency is a rare genetic disorder that affects this critical enzyme within the glycolysis pathway. In recent years, Mitapivat (MTPV, AG-348) has emerged as a notable allosteric activator for treating PK deficiency.
Xiao Han, Tatyana Sandalova, Cheng Zhang, Adil Mardinoglu, Adnane Achour, Renhua Sun   +5 more
doaj   +1 more source

Mapping the Intramolecular Communications among Different Glutamate Dehydrogenase States Using Molecular Dynamics

Biomolecules, 2021
Glutamate dehydrogenase (GDH) is a ubiquitous enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate. It acts as an important branch-point enzyme between carbon and nitrogen metabolisms.
Shaherin Basith, Balachandran Manavalan, Tae Hwan Shin, Gwang Lee   +3 more
doaj   +1 more source

Dual‐Selective Terahertz‐Nanodisc Metasurfaces for Exploring Neurotransmitter Dynamics beyond Spectral Limitations

Advanced Materials, EarlyView.
Observation and detection of neurotransmitter dynamics in aqueous system has been hurdle for analytical fields due to its weak and reversible nature. A terahertz‐nanodisc metasurface which implements comprehensive detection over conformational change and selective sensing is introduced. Utilizing the THz regime photonics and biomimetic environment with
Taeyeon Kim, Yeon Kyung Lee, Yeeun Roh, Jaehun Park, Yong‐Sang Ryu, Hyun Seok Song, Minah Seo   +6 more
wiley   +1 more source

Biophysical assay for tethered signaling reactions reveals tether-controlled activity for the phosphatase SHP-1 [PDF]

, 2017
Tethered enzymatic reactions are ubiquitous in signaling networks but are poorly understood. A previously unreported mathematical analysis is established for tethered signaling reactions in surface plasmon resonance (SPR).
Allard, Jun, Bridge, Marcus, Coker-Gordon, Nicola, Dushek, Omer, Goyette, Jesse, Isaacson, Samuel A., Salas, Citlali Solis   +6 more
core   +1 more source

Bilirubin Targeting WNK1 to Alleviate NLRP3‐Mediated Neuroinflammation

Advanced Science, EarlyView.
At physiological concentrations, bilirubin binds to the kinase domain of WNK1, thereby augmenting its activity and facilitating the phosphorylation of downstream SPAK/OSR1. This phosphorylation inhibits KCC2 activity, leading to elevate intracellular chloride levels in neurons.
Linfei Mao, Jiayu Lu, Quanjun Yang, Zhenqi Liu, Cuiping Wu, Bingbing Ke, Kaiyan Su, Haolin Yuan, Yaqi Cui, Yao Wang, Richard Salvi, Guang Yang, Shankai Yin, Feng Liu, Chunyan Li   +14 more
wiley   +1 more source

Identification of new allosteric sites and modulators of AChE through computational and experimental tools

Journal of Enzyme Inhibition and Medicinal Chemistry, 2018
Allosteric sites on proteins are targeted for designing more selective inhibitors of enzyme activity and to discover new functions. Acetylcholinesterase (AChE), which is most widely known for the hydrolysis of the neurotransmitter acetylcholine, has a ...
Carlos Roca, Carlos Requena, Víctor Sebastián-Pérez, Sony Malhotra, Chris Radoux, Concepción Pérez, Ana Martinez, Juan Antonio Páez, Tom L. Blundell, Nuria E. Campillo   +9 more
doaj   +1 more source

The leucyl aminopeptidase from Helicobacter pylori is an allosteric enzyme.

Microbiology, 2005
This study describes the cloning, genetic analysis and biochemical characterization of a leucyl aminopeptidase (LAP) from Helicobacter pylori. A gene encoding LAP was cloned from H.
Lei Dong, Ni Cheng, Ming-Wei Wang, Junfeng Zhang, Chang Shu, De-xu Zhu   +5 more
semanticscholar   +1 more source

The Use of Isomeric Testosterone Dimers to Explore Allosteric Effects in Substrate Binding to Cytochrome P450 CYP3A4 [PDF]

, 2016
: Cytochrome P450 CYP3A4 is the main drug-metabolizing enzyme in the human liver, being responsible for oxidation of 50% of all pharmaceuticals metabolized by human P450 enzymes.
Bastien, Dominic, Bérubé, Gervais, Denisov, Ilia G., Grinkova, Yelena V., Kincaid, James R., Mak, Piotr J., Sligar, Stephen G.   +6 more
core   +2 more sources

Engineering Electron Transfer Flux between Cytochrome P450 Enzyme and P450 Reductase to Enhance Serotonin Production in Escherichia Coli

Advanced Science, EarlyView.
The electron transfer flux in CPR‐P450 catalytic system is systematically engineered through: i) enhancing electron transfer rate by redesigning the putative electron transfer pathway of CPR; ii) improving electron‐receiving rate by evolving the heme domain of tryptophan‐5‐hydroxylase (T5H); iii) enlarging electron supply by fine‐tuning NADPH synthesis.
Wenzhao Xu, Pengling Wei, Lirong Chen, Ling Gao, Xiaole Xia   +4 more
wiley   +1 more source

Divalent cations in human liver pyruvate kinase exemplify the combined effects of complex-equilibrium and allosteric regulation

Scientific Reports, 2023
There is growing recognition that the functional outcome of binding of an allosteric regulator to a protein/enzyme is influenced by the presence of other ligands.
Tyler A. Martin, Aron W. Fenton
doaj   +1 more source