Results 21 to 30 of about 57,293 (304)

Rational engineering of enzyme allosteric regulation through sequence evolution analysis.

PLoS Computational Biology, 2012
Control of enzyme allosteric regulation is required to drive metabolic flux toward desired levels. Although the three-dimensional (3D) structures of many enzyme-ligand complexes are available, it is still difficult to rationally engineer an ...
Jae-Seong Yang, Sang Woo Seo, Sungho Jang, Gyoo Yeol Jung, Sanguk Kim   +4 more
doaj   +1 more source

Molecular basis for the allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex

Nature Communications, 2021
The allosteric regulation of the bienzyme complex imidazole glycerol phosphate synthase (HisFH) remains to be elucidated. Here, the authors provide structural insights into the dynamic allosteric mechanism by which ligand binding to the cyclase and ...
Jan Philip Wurm, Sihyun Sung, Andrea Christa Kneuttinger, Enrico Hupfeld, Reinhard Sterner, Matthias Wilmanns, Remco Sprangers   +6 more
doaj   +1 more source

Modeling amperometric biosensors based on allosteric enzymes

Computational Science and Techniques, 2013
Computational modeling of a biosensor with allosteric enzyme layer was investigated in this study. The operation of the biosensor is modeled using non-stationary reaction-diffusion equations.
Liutauras Ričkus
doaj   +1 more source

Single-subunit allostery in the kinetics of peptide phosphorylation by protein kinase A; pp. 247–254 [PDF]

Proceedings of the Estonian Academy of Sciences, 2008
Allosteric cooperativity between peptide and ATP binding sites on cAMP-dependent protein kinase catalytic subunit was studied kinetically for the reaction of phosphorylation of seven peptide substrates.
Aleksei Kuznetsov, Jaak Järv
doaj   +1 more source

Different Structures—Similar Effect: Do Substituted 5-(4-Methoxyphenyl)-1H-indoles and 5-(4-Methoxyphenyl)-1H-imidazoles Represent a Common Pharmacophore for Substrate Selective Inhibition of Linoleate Oxygenase Activity of ALOX15?

Molecules, 2023
Mammalian 15-lipoxygenases (ALOX15) are lipid peroxidizing enzymes that exhibit variable functionality in different cancer and inflammation models. The pathophysiological role of linoleic acid- and arachidonic acid-derived ALOX15 metabolites rendered ...
Alexander Zhuravlev, Alejandro Cruz, Vladislav Aksenov, Alexey Golovanov, José M. Lluch, Hartmut Kuhn, Àngels González-Lafont, Igor Ivanov   +7 more
doaj   +1 more source

Glutamine Hydrolysis by Imidazole Glycerol Phosphate Synthase Displays Temperature Dependent Allosteric Activation

Frontiers in Molecular Biosciences, 2018
The enzyme imidazole glycerol phosphate synthase (IGPS) is a model for studies of long-range allosteric regulation in enzymes. Binding of the allosteric effector ligand N'-[5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide ...
George P. Lisi, Allen A. Currier, J. Patrick Loria, J. Patrick Loria   +3 more
doaj   +1 more source

Dabrafenib, idelalisib and nintedanib act as significant allosteric modulator for dengue NS3 protease.

PLoS ONE, 2021
Dengue virus (DENV) encodes a unique protease (NS3/NS2B) essential for its maturation and infectivity and, it has become a key target for anti-viral drug design to treat dengue and other flavivirus related infections.
R V Sriram Uday, Rajdip Misra, Annaram Harika, Sandip Dolui, Achintya Saha, Uttam Pal, V Ravichandiran, Nakul C Maiti   +7 more
doaj   +1 more source

Probing the Sophisticated Synergistic Allosteric Regulation of Aromatic Amino Acid Biosynthesis in Mycobacterium tuberculosis Using ᴅ-Amino Acids. [PDF]

PLoS ONE, 2016
Chirality plays a major role in recognition and interaction of biologically important molecules. The enzyme 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (DAH7PS) is the first enzyme of the shikimate pathway, which is responsible for the synthesis
Sebastian Reichau, Nicola J Blackmore, Wanting Jiao, Emily J Parker   +3 more
doaj   +1 more source

Probing the activity modification space of the cysteine peptidase cathepsin K with novel allosteric modifiers.

PLoS ONE, 2014
Targeting allosteric sites is gaining increasing recognition as a strategy for modulating the activity of enzymes, especially in drug design. Here we investigate the mechanisms of allosteric regulation of cathepsin K as a representative of cysteine ...
Marko Novinec, Brigita Lenarčič, Antonio Baici   +2 more
doaj   +1 more source

Dynamic energy correlation analysis of E. coli aspartokinase III and alteration of allosteric regulation by manipulating energy transduction pathways

Engineering in Life Sciences, 2021
Conformational change associated with allosteric regulation in a protein is ultimately driven by energy transformation. However, little is known about the latter process.
Shizhen Wang, Chengwei Ma, An‐Ping Zeng   +2 more
doaj   +1 more source