Results 81 to 90 of about 2,040,864 (360)

Conversion of Non-allosteric Pyruvate Kinase Isozyme into an Allosteric Enzyme by a Single Amino Acid Substitution*

Journal of Biological Chemistry, 1997
Pyruvate kinase M1, a non-allosteric isozyme, was converted into an allosteric enzyme by replacement of an amino acid in the intersubunit contact. The substitution of Ala-398 with Arg resulted in the pronounced allosteric enzyme. The Hill coefficient and
Yoshitaka Ikeda, Takehiko Tanaka, T. Noguchi   +2 more
semanticscholar   +1 more source

Stochastic approach to molecular interactions and computational theory of metabolic and genetic regulations

, 2006
Binding and unbinding of ligands to specific sites of a macromolecule are one of the most elementary molecular interactions inside the cell that embody the computational processes of biological regulations.
Ackers, Berg, Bintu, Bliss, Bolouri, Chen, Fell, Freire, Gardner, Gibson, Gillespie, Gillespie, H. Kimura, H. Okano, Heinrich, Istrail, King, Kitano, Mackey, Mochizuki, Monod, Morris, Munsky, Ozbudak, Pan, Perutz, R.J. Tanaka, Santillan, Savageau, Segel, Tanaka, van Kampen, Wolf, Wong, Wyman, Zaslaver   +35 more
core   +1 more source

A nucleotide‐independent, pan‐RAS‐targeted DARPin elicits anti‐tumor activity in a multimodal manner

Molecular Oncology, EarlyView.
We report a Designed Ankyrin Repeat Protein that binds and inhibits RAS proteins, which serve as central cell signaling hubs and are essential for the progression of many cancers. Its unique feature is that it does not discriminate between different RAS isoforms or mutations and is capable of binding to RAS in both its active (GTP‐bound) and inactive ...
Jonas N. Kapp, Wouter P. R. Verdurmen, Jonas V. Schaefer, Kari Kopra, Gabriela Nagy‐Davidescu, Elodie Richard, Marie‐Julie Nokin, Patrick Ernst, Rastislav Tamaskovic, Martin Schwill, Ralph Degen, Claudia Scholl, David Santamaria, Andreas Plückthun   +13 more
wiley   +1 more source

A Substrate-induced Biotin Binding Pocket in the Carboxyltransferase Domain of Pyruvate Carboxylase [PDF]

, 2013
Biotin-dependent enzymes catalyze carboxyl transfer reactions by efficiently coordinating multiple reactions between spatially distinct active sites. Pyruvate carboxylase (PC), a multifunctional biotin-dependent enzyme, catalyzes the bicarbonate- and ...
Lietzan, Adam D, Maurice, Martin St.
core   +2 more sources

Transmembrane Activation of Catalysis and Protein Refolding in Synthetic Cells by Enzymes and Nanozymes

Advanced Functional Materials, EarlyView.
Synthetic cells are engineered herein to respond to an external chemical messenger by the activation of intracellular catalysis. The chemical messenger molecules are catalytically generated by an extracellular enzyme or a mineral surface, whereas the intracellular catalysis emerges via direct enzyme activation or via protein refolding.
Dante G. Andersen, Andreas B. Pedersen, Mireia Casanovas Montasell, Søren Moesgaard, Anja Bille Bohn, Alexander N. Zelikin   +5 more
wiley   +1 more source

Deciphering the allosteric regulation of mycobacterial inosine-5′-monophosphate dehydrogenase

Nature Communications
Allosteric regulation of inosine 5′-monophosphate dehydrogenase (IMPDH), an essential enzyme of purine metabolism, contributes to the homeostasis of adenine and guanine nucleotides. However, the precise molecular mechanism of IMPDH regulation in bacteria
Ondřej Bulvas, Zdeněk Knejzlík, Jakub Sýs, Anatolij Filimoněnko, Monika Čížková, Kamila Clarová, Dominik Rejman, Tomáš Kouba, Iva Pichová   +8 more
doaj   +1 more source

PCR-like cascade reactions in the context of an allosteric enzyme mimic.

Journal of the American Chemical Society, 2008
A supramolecular allosteric catalyst that exhibits a PCR-like cascade reaction is reported. The complex is triggered by a reaction with an acetate ion, which turns on a catalytic cascade that exponentially increases acetate ion concentration through an ...
H. Yoon, C. Mirkin
semanticscholar   +1 more source

Fractal mechanisms for the allosteric effects of proteins and enzymes

Biophysical Journal, 1990
Investigations have been made on the reaction kinetics of proteins and enzymes by the statistical methods of random walks on fractal structures. From the point of view of networks of Trap (R) and Free (T) sites, the relationships between the Hill coefficients (h) and the fractal (df) as well as spectral (ds) dimensions of protein molecules are obtained.
Houqiang Li, Haming Zhao, Shuhua Chen
openaire   +3 more sources

ATP allosterically activates the human 5-lipoxygenase molecular mechanism of arachidonic acid and 5(S)-hydroperoxy-6(E),8(Z),11(Z),14(Z)-eicosatetraenoic acid. [PDF]

, 2014
5-Lipoxygenase (5-LOX) reacts with arachidonic acid (AA) to first generate 5(S)-hydroperoxy-6(E),8(Z),11(Z),14(Z)-eicosatetraenoic acid [5(S)-HpETE] and then an epoxide from 5(S)-HpETE to form leukotriene A4, from a single polyunsaturated fatty acid ...
Barbour, Shannon R, Hixon, Mark S, Holman, Theodore R, Smyrniotis, Christopher J, Xia, Zexin   +4 more
core   +2 more sources

DNA‑Directed Assembly of Photonic Nanomaterials for Diagnostic and Therapeutic Applications

Advanced Materials, EarlyView.
DNA‐directed assembly offers a powerful strategy for constructing structured photonic nanomaterials with precise spatial control. This review provides a comprehensive overview of recent advancements in DNA‐assembled photonic nanomaterials for diagnostics and therapeutics, highlighting key design principles, functionalization strategies, and optical ...
Longjiang Ding, Bing Liu, Andreas Peil, Sisi Fan, Jie Chao, Na Liu   +5 more
wiley   +1 more source