Results 11 to 20 of about 2,394,698 (264)

Toward the use of novel alternative methods in epilepsy modeling and drug discovery [PDF]

Frontiers in Neurology, 2023
Epilepsy is a chronic brain disease and, considering the amount of people affected of all ages worldwide, one of the most common neurological disorders.
Claudia Miguel Sanz, Miriam Martinez Navarro, Daniel Caballero Diaz, Gentzane Sanchez-Elexpuru, Vincenzo Di Donato   +4 more
doaj   +2 more sources

Alternative Non-Mammalian Animal and Cellular Methods for the Study of Host–Fungal Interactions

Journal of Fungi, 2023
In the study of fungal pathogenesis, alternative methods have gained prominence due to recent global legislation restricting the use of mammalian animals in research.
Ana Marisa Fusco-Almeida, Samanta de Matos Silva, Kelvin Sousa dos Santos, Marcos William de Lima Gualque, Carolina Orlando Vaso, Angélica Romão Carvalho, Kaila Petrolina Medina-Alarcón, Ana Carolina Moreira da Silva Pires, Jenyffie Araújo Belizario, Lígia de Souza Fernandes, Andrei Moroz, Luis R. Martinez, Orville Hernandez Ruiz, Ángel González, Maria José Soares Mendes-Giannini   +14 more
doaj   +3 more sources

Evaluation and Calibration of Alternative Methods for Estimating Reference Evapotranspiration in the Senegal River Basin

Hydrology, 2020
Reference evapotranspiration (ET0) is a key element of the water cycle in tropical areas for the planning and management of water resources, hydrological modeling, and irrigation management.
Papa Malick Ndiaye, Ansoumana Bodian, Lamine Diop, Abdoulaye Deme, Alain Dezetter, Koffi Djaman   +5 more
doaj   +3 more sources

Alternative Methods for Skin-Sensitization Assessment [PDF]

Toxics, 2022
Inga Mrzyk, Joanna Kalka, Anna Daniel-Wójcik   +2 more
exaly   +2 more sources

A human 3D immune competent full-thickness skin model mimicking dermal dendritic cell activation

Frontiers in Immunology, 2023
We have integrated dermal dendritic cell surrogates originally generated from the cell line THP-1 as central mediators of the immune reaction in a human full-thickness skin model.
Johanna Maria Hölken, Katja Friedrich, Marion Merkel, Nelli Blasius, Ursula Engels, Timo Buhl, Karsten Rüdiger Mewes, Lars Vierkotten, Nicole Elisabeth Teusch   +8 more
doaj   +1 more source

Human-Relevant Sensitivity of iPSC-Derived Human Motor Neurons to BoNT/A1 and B1

Toxins, 2021
The application of botulinum neurotoxins (BoNTs) for medical treatments necessitates a potency quantification of these lethal bacterial toxins, resulting in the use of a large number of test animals.
Maren Schenke, Hélène-Christine Prause, Wiebke Bergforth, Adina Przykopanski, Andreas Rummel, Frank Klawonn, Bettina Seeger   +6 more
doaj   +1 more source

Toxic Indoor Air Is a Potential Risk of Causing Immuno Suppression and Morbidity—A Pilot Study

Journal of Fungi, 2022
We aimed to establish an etiology-based connection between the symptoms experienced by the occupants of a workplace and the presence in the building of toxic dampness microbiota. The occupants (5/6) underwent a medical examination and urine samples (2/6)
Kirsi Vaali, Marja Tuomela, Marika Mannerström, Tuula Heinonen, Tamara Tuuminen   +4 more
doaj   +1 more source

Validation of a Novel Double Control Quantitative Copy Number PCR Method to Quantify Off-Target Transgene Integration after CRISPR-Induced DNA Modification

Methods and Protocols, 2022
In order to improve a recently established cell-based assay to assess the potency of botulinum neurotoxin, neuroblastoma-derived SiMa cells and induced pluripotent stem-cells (iPSC) were modified to incorporate the coding sequence of a reporter ...
Brit-Maren Michaud Schjeide, Maren Schenke, Bettina Seeger, Gerhard Paul Püschel   +3 more
doaj   +1 more source

Antibacterial Activity of the Pyrogallol against Staphylococcus aureus Evaluated by Optical Image

Biologics, 2022
The minimum inhibitory concentration (MIC) is used to define the lowest concentration at which a substance can inhibit bacterial growth. This study aimed to evaluate the MIC of pyrogallol against Staphylococcus aureus and to propose a method for building
Lígia C. C. Oliveira, Francisco A. A. Rodrigues, Cristina Rodrigues dos Santos Barbosa, Joycy Francely Sampaio dos Santos, Nair Silva Macêdo, Zildene de Sousa Silveira, Henrique Douglas Melo Coutinho, Francisco Assis Bezerra da Cunha   +7 more
doaj   +1 more source

Qualitative methods: an alternative view [PDF]

BMJ, 2011
Are qualitative and quantitative research really based on different philosophies? John Paley and Richard Lilford argue that the idea that qualitative research is constructivist should not be allowed to diffuse into medical literature unopposed.
Paley, John, Lilford, Richard J
openaire   +3 more sources