Results 11 to 20 of about 2,389,287 (216)

Alternative Non-Mammalian Animal and Cellular Methods for the Study of Host–Fungal Interactions

Journal of Fungi, 2023
In the study of fungal pathogenesis, alternative methods have gained prominence due to recent global legislation restricting the use of mammalian animals in research.
Ana Marisa Fusco-Almeida, Samanta de Matos Silva, Kelvin Sousa dos Santos, Marcos William de Lima Gualque, Carolina Orlando Vaso, Angélica Romão Carvalho, Kaila Petrolina Medina-Alarcón, Ana Carolina Moreira da Silva Pires, Jenyffie Araújo Belizario, Lígia de Souza Fernandes, Andrei Moroz, Luis R. Martinez, Orville Hernandez Ruiz, Ángel González, Maria José Soares Mendes-Giannini   +14 more
doaj   +1 more source

Impact of mono-culture vs. Co-culture of keratinocytes and monocytes on cytokine responses induced by important skin sensitizers

Journal of Immunotoxicology, 2021
Sensitization to a contact allergen brings with it a lifelong risk to develop allergic contact dermatitis. Inflammation is an important part of the skin sensitizing mechanism, and understanding how different haptens stimulate the immune system, as well ...
Venkatanaidu Karri, Carola Lidén, Nanna Fyhrquist, Johan Högberg, Hanna L. Karlsson   +4 more
doaj   +1 more source

Avaliação retrospectiva dos testes de toxicidade inespecífica realizados no Instituto Nacional de Controle de Qualidade em Saúde em produtos biológicos

Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia, 2021
Introdução:O teste de toxicidade in vivo, conhecido como Teste de Toxicidade Inespecífica (TTI), é sugerido para avaliar a segurança de produtos biológicos e derivados de biotecnologia.
Lilia Serodio, Cristiane Caldeira, Carolina Oliveira, Elias de Jesus, Wlamir Moura, Octavio Presgrave   +5 more
doaj  

Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth

BioTechniques, 2021
Routine cell culture demands the use of animal-derived products, mainly fetal bovine serum and swine or bovine trypsin. According to the 3Rs principle and to the European Centre for the Validation of Alternative Methods, animal-free substitutes are ...
Mara Fusi, Silvia Dotti
doaj   +1 more source

T-2 Toxin-Induced Hepatotoxicity in HepG2 Cells Involves the Inflammatory and Nrf2/HO-1 Pathways

Toxins
The T-2 toxin is one of the most toxic mycotoxins, to which the population is exposed through the diet. T-2 toxins are especially found in cereals and cereal-based products.
Mercedes Taroncher, Felipe Franco-Campos, Yelko Rodríguez-Carrasco, María-José Ruiz   +3 more
doaj   +1 more source

Numerical procedure for estimating temperature in solarized soils Procedimento numérico para estimar temperaturas de solos solarizados

Pesquisa Agropecuária Brasileira, 2006
The objective of this work was to develop a simplified numerical procedure for the estimation of accumulated monthly hours of solarized soil temperatures.
Chigueru Tiba, Raquel Ghini
doaj   +1 more source

Evaluation and Calibration of Alternative Methods for Estimating Reference Evapotranspiration in the Senegal River Basin

Hydrology, 2020
Reference evapotranspiration (ET0) is a key element of the water cycle in tropical areas for the planning and management of water resources, hydrological modeling, and irrigation management.
Papa Malick Ndiaye, Ansoumana Bodian, Lamine Diop, Abdoulaye Deme, Alain Dezetter, Koffi Djaman   +5 more
doaj   +1 more source

Establishing a 3D Spheroid Model of Cholinergic Neurons from SH-SY5Y Cells for Neurotoxicity Assessment

Toxins
The nervous system maintains homeostasis and coordinated behavior through complex neuronal and glial cells. Traditional models, such as primary rodent neurons and human-induced pluripotent stem cell (hIPSC)-derived neurons, have advanced our ...
Felipe Franco-Campos, Mónica Fernández-Franzón, Yelko Rodríguez-Carrasco, María-José Ruiz   +3 more
doaj   +1 more source

The Growing Importance of Three-Dimensional Models and Microphysiological Systems in the Assessment of Mycotoxin Toxicity

Toxins, 2023
Current investigations in the field of toxicology mostly rely on 2D cell cultures and animal models. Although well-accepted, the traditional 2D cell-culture approach has evident drawbacks and is distant from the in vivo microenvironment.
Veronica Zingales, Maria Rosaria Esposito, Noemi Torriero, Mercedes Taroncher, Elisa Cimetta, María-José Ruiz   +5 more
doaj   +1 more source

Differential Proliferation Effect of the Newly Synthesized Valine, Tyrosine and Tryptophan–Naphthoquinones in Immortal and Tumorigenic Cervical Cell Lines

Molecules, 2020
We previously showed that microwave assisted synthesis is the best method for the synthesis of naphthoquinone amino acid and chloride-naphthoquinone amino acid derivatives by a complete evaluation of reaction conditions such as stoichiometry, bases, and ...
Sergio Córdova-Rivas, Jorge Gustavo Araujo-Huitrado, Ernesto Rivera-Avalos, Ismailia L. Escalante-García, Sergio M. Durón-Torres, Yamilé López-Hernández, Hiram Hernández-López, Lluvia López, Denisse de Loera, Jesús Adrián López   +9 more
doaj   +1 more source