Results 101 to 110 of about 87,604,756 (316)

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

AI redefine untargeted metabolomics: estimating chemical amounts for a Human Exposome Project

Frontiers in Public Health
The Human Exposome Project aims to map the totality of environmental exposures, but its success relies on transforming qualitative detections into quantitative data.
Fenna C. M. Sillé, Karolina Kopańska, Carsten Prasse, Carsten Prasse, Thomas Luechtefeld, Thomas Luechtefeld, Thomas Hartung, Thomas Hartung, Thomas Hartung   +8 more
doaj   +1 more source

Multiparametric Material Functionality of Microtissue-Based In Vitro Models as Alternatives to Animal Testing. [PDF]

Adv Sci (Weinh), 2022
Stengelin E, Thiele J, Seiffert S.
europepmc   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Advice for bad computational toxicologists

NAM Journal
We recently gave advice for bad (traditional) toxicologists (Rabbit, 2025)一but we also have advice for the new breed of computational toxicologists. So many ways to do a lousy job…
Srijit Seal, Richard R. Rabbit (aka Thomas Hartung)   +1 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Impact of bioactive substances on the gastrointestinal tract and performance of weaned piglets: a review [PDF]

, 2009
The EU ban on in-feed antibiotics has stimulated research on weaning diets as a way of reducing post-weaning gut disorders and growth check in pigs. Many bioactive components have been investigated but only few have shown to be effective.
D. Torrallardona, S. J. Koopmans, Lalles, J.P., Janczyk, P., Koopmans, S. J., S.J. Koopmans, P. Janczyk, Lallès, J. P., Torrallardona, D., Bosi, P., Koopmans, S.J., P. Bosi, BOSI, PAOLO, Torrallardona, David, J.P. Lallès, J. P. Lallès   +15 more
core   +1 more source

AI redefines mass spectrometry chemicals identification: retention time prediction in metabolomics and for a Human Exposome Project

Frontiers in Public Health
The comprehensive identification of environmental and endogenous chemicals in human biospecimens is a critical bottleneck for realizing the Human Exposome Project.
Fenna C. M. Sillé, Carsten Prasse, Carsten Prasse, Thomas Luechtefeld, Thomas Luechtefeld, Thomas Hartung, Thomas Hartung   +6 more
doaj   +1 more source

Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers

FEBS Letters, EarlyView.
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui, Agnes Banreti, Armelle L. Ernst, Guillaume Leseigneur, Uwe J. Meierhenrich   +4 more
wiley   +1 more source

Spray Dried Animal Plasma as an Alternative to Antibiotics in Weanling Pigs [PDF]

, 2010
Piglet health at weaning is compromised due to several stress factors. Following the ban of antibiotic growth promoters new alternatives are required to control these problems. This paper reviews the evidence available for the use of spray dried animal
Torrallardona, David
core