Results 41 to 50 of about 381,771 (314)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

First result with AMBER+FINITO on the VLTI: The high-precision angular diameter of V3879 Sgr

, 2008
Our goal is to demonstrate the potential of the interferometric AMBER instrument linked with the Very Large Telescope Interferometer (VLTI) fringe-tracking facility FINITO to derive high-precision stellar diameters.
B. Bauvir, F. Rantakyrö, Gai, J.-B. Le Bouquin, M. Schöller, P. Haguenauer, Petrov, Richichi, Richichi, S. Menardi, Tatulli   +10 more
core   +2 more sources

Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity

Molecules, 2022
Parkinson’s disease (PD) is the second most common progressive neurodegenerative disease, after Alzheimer’s disease. In our previous study, we found that amber—a fossilized plant resin—can protect cells from apoptosis by decreasing the generation of ...
Yuening Luo, Siqi Zhou, Reiko Takeda, Kazuma Okazaki, Marie Sekita, Kazuichi Sakamoto   +5 more
doaj   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Development of Hydroxyapatite Coatings for Orthopaedic Implants from Colloidal Solutions: Part 1—Effect of Solution Concentration and Deposition Kinetics

Nanomaterials, 2023
This study introduces and explores the use of supersaturated solutions of calcium and phosphate ions to generate well-defined hydroxyapatite coatings for orthopaedic implants.
Bríd Murphy, Mick A. Morris, Jhonattan Baez   +2 more
doaj   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

Video frame interpolation neural network for 3D tomography across different length scales

Nature Communications
Three-dimensional (3D) tomography is a powerful investigative tool for many scientific domains, going from materials science, to engineering, to medicine.
Laura Gambini, Cian Gabbett, Luke Doolan, Lewys Jones, Jonathan N. Coleman, Paddy Gilligan, Stefano Sanvito   +6 more
doaj   +1 more source

Broad Epitope Coverage of Therapeutic Multi-Antibody Combinations Targeting SARS-CoV-2 Boosts In Vivo Protection and Neutralization Potency to Corner an Immune-Evading Virus

Biomedicines
Therapeutic antibodies (Abs) which act on a broader range of epitopes may provide more durable protection against the genetic drift of a target, typical of viruses or tumors.
Ilse Roodink, Maartje van Erp, Andra Li, Sheila Potter, Sander M. J. van Duijnhoven, Milou Smits, Arthur J. Kuipers, Bert Kazemier, Bob Berkeveld, Ellen van Geffen, Britte S. de Vries, Danielle Rijbroek, Bianca Boers, Sanne Meurs, Wieger Hemrika, Alexandra Thom, Barry N. Duplantis, Roland A. Romijn, Jeremy S. Houser, Jennifer L. Bath, Yasmina N. Abdiche   +20 more
doaj   +1 more source

Characterizing Hydroxyapatite Deposited from Solution onto Novel Substrates in Terms of Growth Mechanism and Physical Chemical Properties

Materials Proceedings, 2023
Bulk titanium and CoCr are the most common metals for use in orthopedic implants, but there are significant advantages in alternative substrates. Research in the last decade has focused on various alternatives; however, these materials are hindered by ...
Bríd Murphy, Jhonattan Baez, Mick A. Morris   +2 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source