Results 21 to 30 of about 359 (138)

Affinity labeling of a sulfhydryl group in the cardiacglycoside receptor site of Na+/K(+)-ATPase by N-hydroxysuccinimidyl derivatives of digoxigenin.

open access: yesEuropean Journal of Biochemistry, 1995
Na+/K(+)-ATPase from pig kidney is inactivated by protein-reactive N-hydroxysuccinimidyl derivatives of digoxigenin. Like digoxigenin, its protein-reactive derivatives N-hydroxysuccinimidyl digoxigenin-3-methylcarbonyl-epsilon-aminocaproate (HDMA), 3 ...
R. Antolović   +3 more
semanticscholar   +1 more source

On the location and structure of the active sites of antibody molecules.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1968
By the method of affinity-labeling1 it has been possible to attach chemical tags to amino acid residues that, by a number of stringent criteria, are contact residues within the active sites of antibody (Ab) molecules.2' With IgG Ab specific to three ...
BY S. J. Singer, And, Neal 0. THORPEt
semanticscholar   +1 more source

The heterogeneity of combining sites of anti-benzylpenicilloyl antibodies obtained from individual rabbits: fractionation of antibodies with a specific immunoadsorbent.

open access: yesJournal of Immunology, 1967
Benzylpenicilloyl (BPO) groups were introduced into the bisdiazotized benzidine treated insoluble bovine γ-globulin (BGG) aggregates by direct coupling of benzylpenicillin in alkaline condition.
T. Atsumi   +4 more
semanticscholar   +1 more source

Chick heart cells with high intracellular calcium concentration have a higher affinity for cardiac glycosides than those with low intracellular calcium concentration, as revealed by affinity labelling with a digoxigenin derivative.

open access: yesEuropean Journal of Biochemistry, 1992
Digital-imaging fluorescence microscopy with fura-2 allows the determination of intracellular calcium concentration ([Ca2+]i) in single cells. At a cell density of 10(5) cells/petri dish 44% of the chick embryo heart cells had a high [Ca2+]i of 99.4 ...
Barbara Ahlemeyer   +3 more
semanticscholar   +1 more source

The heterogeneity of combining sites of anti-benzylpenicilloyl antibodies obtained from individual rabbits: changes in combining sites of gammaG and gammaM antibodies during the immune response.

open access: yesJournal of Immunology, 1968
Nine rabbits were immunized with benzylpenicilloyl-human γG conjugate (BPO-HGG). Radioiodinated γG and γM were prepared from the anti-BPO-HGG sera of individual rabbits collected 10 days and 10 weeks after the first immunization.
T. Atsumi   +4 more
semanticscholar   +1 more source

The binding reactions of antibodies specific for the 2,6-dinitrophenyl group.

open access: yesJournal of Immunology, 1969
Rabbit and guinea pig antibodies specific for the 2,6-DNP group were isolated from sera by specific precipitation and hapten elution. Anti-2,6-DNP-antibodies formed late in the immune response displayed greater cross-reactivity with 2,4-DNP and 2,4,6 ...
J. R. Little   +2 more
semanticscholar   +1 more source

Hyperglycemic effect of amino compounds structurally related to caproate in rats.

open access: yesJournal of NutriLife, 1975
The chronic feeding of small amounts (0.3-3% of diet weight) of certain amino derivatives of caproate resulted in hyperglycemia, an elevated glucose tolerance curve and, occasionally, glucosuria.
W. O. Caster, S. Bleecker
semanticscholar   +1 more source

Diffusion of Epsilon Aminocaproic Acid to the Joints

open access: yesExperimental Biology and Medicine, 1970
SummaryThe concentration of E-ACA in the joint fluid and joint capsule was measured at different intervals after iv injection of the substance in a dose of 0.1 g/kg body weight in 16 patients undergoing operation because of a ruptured meniscus of the knee joint. It was found that E-ACA diffuses to both of the above-mentioned tissues and that some E-ACA
openaire   +3 more sources

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