Direct asymmetric synthesis of β-branched aromatic α-amino acids using engineered phenylalanine ammonia lyases [PDF]
Nature Communicationsβ-Branched aromatic α-amino acids are valuable building blocks in natural products and pharmaceutically active compounds. However, their chemical or enzymatic synthesis is challenging due to the presence of two stereocenters.
Chenghai Sun +12 more
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Programmable Polyproteams of Tyrosine Ammonia Lyases as Cross-Linked Enzymes for Synthesizing p-Coumaric Acid [PDF]
Biomolecules, 2022 Ideal immobilization with enhanced biocatalyst activity and thermostability enables natural enzymes to serve as a powerful tool to yield synthetically useful chemicals in industry. Such an enzymatic method strategy becomes easier and more convenient with
Mingyu Jia +7 more
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Towards a general approach for tailoring the hydrophobic binding site of phenylalanine ammonia-lyases [PDF]
Scientific Reports, 2022 Unnatural substituted amino acids play an important role as chiral building blocks, especially for pharmaceutical industry, where the synthesis of chiral biologically active molecules still represents an open challenge.
Souad Diana Tork +6 more
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Saturation Mutagenesis for Phenylalanine Ammonia Lyases of Enhanced Catalytic Properties [PDF]
Biomolecules, 2020 Phenylalanine ammonia-lyases (PALs) are attractive biocatalysts for the stereoselective synthesis of non-natural phenylalanines. The rational design of PALs with extended substrate scope, highlighted the substrate specificity-modulator role of residue ...
Raluca Bianca Tomoiagă +5 more
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Expression of phenylalanine ammonia lyases in Synechocystis sp. PCC 6803 and subsequent improvements of sustainable production of phenylpropanoids [PDF]
Microbial Cell Factories, 2022 Background Phenylpropanoids represent a diverse class of industrially important secondary metabolites, synthesized in plants from phenylalanine and tyrosine.
Kateryna Kukil, Pia Lindberg
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PRODUCTION OF RECOMBINANT, NON-TAGGED PHENYLALANINE AMMONIA-LYASES EMPLOYING TEV PROTEASE-REMOVABLE AFFINITY TAGS [PDF]
Studia Universitatis Babes-Bolyai Chemia, 2022 Nowadays, protein purification by the aid of affinity tags can be carried out with high speed and efficiency. However, in several cases, affinity tags can significantly alter the key properties of enzymes, especially activity and/or thermostability ...
Alina FILIP +4 more
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Sustainable synthesis of L-phenylalanine derivatives in continuous flow by immobilized phenylalanine ammonia lyase [PDF]
Frontiers in Catalysis, 2023 The application of phenylalanine ammonia lyases (PALs) for the amination of a variety of cinnamic acids has been shown to be a cost-efficient method to produce a variety of phenylalanine analogues.
David Roura Padrosa +3 more
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Highly Active and Specific Tyrosine Ammonia-Lyases from Diverse Origins Enable Enhanced Production of Aromatic Compounds in Bacteria and Saccharomyces cerevisiae. [PDF]
Appl Environ Microbiol, 2015 Phenylalanine and tyrosine ammonia-lyases form cinnamic acid and p-coumaric acid, which are precursors of a wide range of aromatic compounds of biotechnological interest.
Jendresen CB +8 more
europepmc +3 more sources
STRUCTURAL MODELING OF PHENYLALANINE AMMONIA-LYASES AND RELATED MIO-CONTAINING ENZYMES – AN INSIGHT INTO THERMOSTABILITY AND IONIC INTERACTIONS [PDF]
Studia Universitatis Babes-Bolyai Chemia, 2015 Biocatalysis and bioinformatics are powerful tools to analyze and enhance the properties of biocatalysts for industrial technologies. In this work, the three-dimensional structures of various phenylalanine ammonia-lyases and other 5-methylene-3,5 ...
Gergely BÁNÓCZI +5 more
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Enhancing Caffeic Acid Production in Escherichia coli Through Heterologous Enzyme Combinations and Semi-Rational Design [PDF]
MetabolitesBackground/Objectives: Caffeic acid is a hydroxycinnamic acid that has a wide range of applications in the medical field. The synthesis of caffeic acid using microbial fermentation technology is an environmentally friendly method.
Qing Luo +7 more
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