Expression and Properties of the Highly Alkalophilic Phenylalanine Ammonia-Lyase of Thermophilic Rubrobacter xylanophilus [PDF]
, 2014 The sequence of a phenylalanine ammonia-lyase (PAL; EC: 4.3.1.24) of the thermophilic and radiotolerant bacterium Rubrobacter xylanophilus (RxPAL) was identified by screening the genomes of bacteria for members of the phenylalanine ammonia-lyase family.
Bánóczi, Gergely +9 more
core +2 more sources
A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS [PDF]
, 2017 This study describes cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from ...
Adriana MARCOVICI +8 more
core +2 more sources
Biocatalysis as Useful Tool in Asymmetric Synthesis: An Assessment of Recently Granted Patents (2014–2019) [PDF]
, 2019 The broad interdisciplinary nature of biocatalysis fosters innovation, as different technical fields are interconnected and synergized. A way to depict that innovation is by conducting a survey on patent activities.
Alcántara, Andrés R. +2 more
core +3 more sources
PLoS ONE, 2020 The enzymatic reactions leading to the deamination of β-lysine, lysine, or 2-aminoadipic acid are of great interest for the metabolic conversion of lysine to adipic acid. Enzymes able to carry out these reactions are not known, however ammonia lyases (EC
Veronica Saez-Jimenez +7 more
doaj +1 more source
Phenylpropanoid Pathway Engineering: An Emerging Approach towards Plant Defense
Pathogens, 2020 Pathogens hitting the plant cell wall is the first impetus that triggers the phenylpropanoid pathway for plant defense. The phenylpropanoid pathway bifurcates into the production of an enormous array of compounds based on the few intermediates of the ...
Vivek Yadav +6 more
doaj +1 more source
Stepwise Simulation of 3,5-Dihydro-5-methylidene-4H-imidazol-4-one (MIO) Biogenesis in Histidine Ammonia-lyase [PDF]
, 2016 A 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO) electrophilic moiety is post-translationally and autocatalytically generated in homotetrameric histidine ammonia-lyase (HAL) and other enzymes containing the tripeptide Ala-Ser-Gly in a suitably ...
Case D. A. +11 more
core +2 more sources
Phenylalanine Ammonia-lyase of Bamboo Shoots [PDF]
Agricultural and Biological Chemistry, 1988 Phenylalanine ammonia-lyase (PAL) was purified from bamboo shoots (Bambusa oldhami Munro) through steps of DEAE-Sephacel column chromatography, Sepharose CL-6B gel filtration, and a second DEAE-Sephacel column chromatography. Polyacrylamide gel electrophoresis showed that the purified enzyme (Mr 360, 000) still contained two minor bands with much ...
Ru-Yin CHEN +2 more
openaire +2 more sources
Microbial Cell Factories, 2020 Background Caffeic acid is industrially recognized for its antioxidant activity and therefore its potential to be used as an anti-inflammatory, anticancer, antiviral, antidiabetic and antidepressive agent.
Kristina Haslinger +1 more
doaj +1 more source
Simulation and prediction of protein production in fed-batch E. coli cultures : an engineering approach [PDF]
, 2016 An overall model describing the dynamic behavior of fed-batch E. coli processes for protein production has been built, calibrated and validated. Using a macroscopic approach, the model consists of three interconnected blocks allowing simulation of ...
Bennett +34 more
core +2 more sources
Enantioselective Intramolecular C-H Amination Catalyzed by Engineered Cytochrome P450 Enzymes In Vitro and In Vivo [PDF]
, 2013 Nitrogen activation: Though P450 enzymes are masters of oxygen activation and insertion into C-H bonds, their ability to use nitrogen for the same purpose has so far not been explored.
Arnold, Frances H. +6 more
core +1 more source