Results 261 to 270 of about 165,809 (296)

Amplicon-based sequencing of viral genomes

openaire   +2 more sources

Capturing sequence ambiguity among taxa in a primer-specific manner to improve taxonomic classification of amplicon sequencing. [PDF]

Nucleic Acids Res
Nearing JT, Thompson KN, Kuntz T, Louyakis AS, Bhosle A, Branck T, Badri DV, Franzosa EA, Brockel C, Huttenhower C, Short MI.   +10 more
europepmc   +1 more source

16S rRNA Gene Amplicon Sequencing of Gut Microbiota in Gestational Diabetes Mellitus and Type 2 Diabetes Mellitus. [PDF]

J Diabetes Res
Qing Y, Zhou H, Zhou C, Song Z, Gao J, Wei J.   +5 more
europepmc   +1 more source

Determining genotype and antimicrobial resistance of Salmonella Typhi in environmental samples by amplicon sequencing. [PDF]

PLoS Negl Trop Dis
Troman C, Horsfield ST, Abraham D, Mohan VR, Giri S, Nair S, Shaw AG, Dyson Z, Holt KE, Grassly NC.   +9 more
europepmc   +1 more source

Marine microbial mock communities for validating rRNA gene amplicon sequencing. [PDF]

Microbiol Resour Announc
Lampe RH, Rabines AJ, Ellman BA, Zheng H, Allen AE.   +4 more
europepmc   +1 more source

Development of a qPCR assay and tremabiome deep amplicon sequencing method for differentiation of fluke species in livestock

Abbas M, Kozel K, Daramola O, Selemetas N, Ali Q, Ashraf S, Ibrahim I, Deza-Cruz I, Fingerhood S, Robinson MW, Morgan ER, Chaudhry U, Betson M.   +12 more
europepmc   +1 more source

Reproducibility and quantitation of amplicon sequencing-based detection [PDF]

ISME Journal, 2011
Abstract To determine the reproducibility and quantitation of the amplicon sequencing-based detection approach for analyzing microbial community structure, a total of 24 microbial communities from a long-term global change experimental site were examined.
Jizhong Zhou, Liyou Wu, Ye Deng
exaly   +3 more sources

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Practical innovations for high-throughput amplicon sequencing

Nature Methods, 2013
We describe improvements for sequencing 16S ribosomal RNA (rRNA) amplicons, a cornerstone technique in metagenomics. Through unique tagging of template molecules before PCR, amplicon sequences can be mapped to their original templates to correct amplification bias and sequencing error with software we provide.
Derek S Lundberg, Scott Yourstone, Piotr A Mieczkowski   +2 more
exaly   +3 more sources

Different Amplicon Targets for Sequencing-Based Studies of Fungal Diversity

Applied and Environmental Microbiology, 2017
Target-gene amplicon sequencing is the most exploited high-throughput sequencing application in microbial ecology. The targets are taxonomically relevant genes with the 16S rRNA being the gold standard for bacteria.
Francesca de Filippis, Manolo Laiola, Giuseppe Blaiotta   +2 more
exaly   +2 more sources

Amplicon Sequencing Pipelines in Metagenomics

2023
Taxonomic profiling among a large number of samples is a fundamental task during amplicon sequencing analysis. The heterogeneity and technical noises in the sample handling, library preparation, and sequencing present a major challenge to how the biological conclusions are drawn from the data analysis, and accordingly, many tools have been developed to
openaire   +2 more sources