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Amplified Fragment Length Polymorphism: Applications and Recent Developments

Methods in molecular biology, 2020
AFLP or amplified fragment length polymorphism is a PCR-based molecular technique that uses selective amplification of a subset of digested DNA fragments from any source to generate and compare unique fingerprints of genomes. It is more efficient in terms of time, economy, reproducibility, informativeness, resolution, and sensitivity, compared to other
Thotten Elampilay, Sheeja   +5 more
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Amplified Fragment Length Polymorphism Analysis

2009
Amplified fragment length polymorphism (AFLP) analysis is a universal polymerase chain reaction (PCR)-based DNA fingerprinting technique comprising three main stages: (i) digestion of genomic DNA with restriction endonucleases and ligation to double-stranded adaptors (each comprised of two oligonucleotides), thus creating restriction fragments with ...
FRY NK, SAVELKOUL PH, VISCA, PAOLO
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Amplified Restriction Fragment Length Polymorphism in Parasite Genetics

Parasitology Today, 2000
The amplified restriction fragment length polymorphism (AFLP) technique is a relatively new method for the analysis of polymorphism that has not yet been widely used in parasitology. In this article, Dan Masiga, Andy Tait and Mike Turner provide a brief introduction to AFLP and illustrate how it can be used in the investigation of marker inheritance in
D K, Masiga, A, Tait, C M, Turner
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An integrated restriction fragment length polymorphism - amplified fragment length polymorphism linkage map for cultivated sunflower

Genome, 2001
Restriction fragment length polymorphism (RFLP) maps have been constructed for cultivated sunflower (Helianthus annuus L.) using three independent sets of RFLP probes. The aim of this research was to integrate RFLP markers from two sets with RFLP markers for resistance gene candidate (RGC) and amplified fragment length polymorphism (AFLP) markers ...
M A, Gedil   +8 more
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Amplified Fragment Length Polymorphism (AFLP) Analysis of Listeria monocytogenes

Systematic and Applied Microbiology, 2000
AFLP analysis using four selective primers was performed on a set of 33 Listeria monocytogenes including strains from patients and foods implicated in outbreaks, human sporadic cases or foods. Strains were tested belonging to serovars 1/2a, 1/2b, 1/2c, 3b, and 4b.
RIPABELLI, Giancarlo   +2 more
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Amplified (Restriction) Fragment Length Polymorphism (AFLP) Analysis

2004
The amplified (restriction) fragment length polymorphism (AFLP) technique is a method for DNA profiling that is now widely applied for assessing diversity among various organisms with varying genomic complexity, from small bacterial to large plant genomes.
Daniel K, Masiga, C Michael R, Turner
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Olive genetic diversity assessed using amplified fragment length polymorphisms

Theoretical and Applied Genetics, 1999
Amplified fragment length polymorphism (AFLP) analysis was used to study the genetic variation within and among populations of genus Olea. A group of genotypes, all of them cultivated varieties of a single species, Olea europaea, was compared with wild olives and with a group of individuals belonging to different Olea species.
ANGIOLILLO, Antonella   +2 more
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Amplified Fragment Length Polymorphism (AFLP)

1998
The AFLP technology (1, 2) recently developed by KEYGENE provides a new powerful tool for the detection and evaluation of genetic variation in germplasm collections and in the screening of biodiversity. Compared with the widely used RFLP technique (3) (see Chapter 6), AFLPs are faster, less labour intensive and provide more information.
Michaela C. Matthes   +2 more
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