Results 301 to 310 of about 191,095 (353)

Amplified Fragment Length Polymorphism (AFLP) Analysis of Listeria monocytogenes

Systematic and Applied Microbiology, 2002
An agarose gel based single enzyme AFLP method using EcoR1 digestion of Listeria monocytogenes DNA was developed for epidemiological typing. The method was evaluated with 84 L. monocytogenes cultures, and results were compared with those obtained with serotyping, phage-typing and cadmium and arsenic resistance typing.
M M, Guerra, F, Bernardo, J, McLauchlin
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Amplified Fragment Length Polymorphism Analysis of Salmonella enterica

2007
Amplified fragment length polymorphism (AFLP) is a powerful PCR-based fingerprinting method and has the capacity to reveal variation around the whole genome by selectively amplifying a subset of restriction fragments for comparison. The restriction fragments analyzed are small, and even mutation of 1 bp can be detected.
Ruiting, Lan, Peter R, Reeves
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HLA-DP typing by amplified fragment length polymorphisms (AFLPs)

Immunogenetics, 1990
Here we present an alternative PCR-based method of HLA-DP typing that involves analysis of electrophoretically separated restriction fragments, or amplified fragment length polymorphisms (AFLPs), produced by digestion of PCR-amplified DPB exons 2 sequences at allele-specific restriction sites.
J W, Dekker, S, Easteal
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Amplified fragment length polymorphism analysis of vandaceous orchids

Plant Science, 1999
Abstract We investigated the application of the PCR-based fingerprinting technique, amplified fragment length polymorphism (AFLP), in orchids. The optimal AFLP patterns have been determined using primer combinations of EcoRI +4 and MseI +3 selective nucleotides. The same reproducible AFLP patterns were demonstrated in genomic DNAs isolated both from:
Chen, X., Hoon Lim, S., Man Wong, S., Hwa Lee, Y., Kuo, J., Wing Yam, T., Lin, J.-J.   +6 more
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Amplified Fragment Length Polymorphism (AFLP) Markers for Barley Malt Fingerprinting

Journal of Cereal Science, 1999
Abstract Barley is the cerealpar excellencein the production of malt for beer and other alcoholic beverages. In this work, biochemical (hordein A-PAGE) and molecular (RAPDs, AFLPs) markers have been compared for their efficiency in malt fingerprinting.
FACCIOLI P., PECCHIONI, Nicola, A. M. STANCA, V. TERZI   +3 more
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Amplified fragment length polymorphism for genetic diversity assessment in globe artichoke

Theoretical and Applied Genetics, 2004
Globe artichoke ( Cynara cardunculus L. var. scolymus L.) is a diploid (2 n=2 x=34), predominantly cross-pollinated plant native to the Mediterranean basin, and Italy contains the richest primary cultivated 'gene pool'. Commercial production is mainly based on perennial cultivation of vegetatively propagated clones that are highly heterozygous and ...
LANTERI, Sergio, SABA E, CADINU M, MALLICA G. M, BAGHINO L, PORTIS, Ezio   +5 more
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Distribution of Amplified Fragment Length Polymorphism D1S80 Alleles in Turkish Population

Journal of Forensic Sciences, 2001
Abstract Blood samples from 112 unrelated healty volunteer and criminal donors in Turkey were collected in EDTA tubes. Liquid blood was deposited onto cotton swatches and dried at room temperature. The stains were stored at −20°C until analyzed. DNA was extracted by Chelex 100 method (1).
Cakir, AH, Kesici, T, Acik, LEYLA
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Amplified fragment length polymorphism versus random amplified polymorphic DNA markers: clonal diversity in Saxifraga cernua

Molecular Ecology, 2003
AbstractRandom amplified polymorphic DNA (RAPD) markers are sensitive to changes in reaction conditions and may express polymorphisms of nongenetic origin. Taxa with variable chromosome numbers are particularly challenging cases, as differences in DNA content may also influence marker reproducibility.
Kjolner, S., Sastad, S. M., Taberlet, P., Brochmann, C.   +3 more
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Estimating Nucleotide Diversity From Random Amplified Polymorphic DNA and Amplified Fragment Length Polymorphism Data

Molecular Phylogenetics and Evolution, 2001
A way to estimate the index of nucleotide diversity (pi) from band match frequencies in random amplified polymorphic DNA and amplified fragment length polymorphism data is described. pi is shown to be a simple function of the proportion of mismatched bands between two individuals drawn at random from a population (phi) and the number of discriminating ...
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DNA Extraction Strategies for Amplified Fragment Length Polymorphism Analysis

Journal of Forensic Sciences, 1994
Abstract A polymerase chain reaction-based DNA typing method, amplified fragment length polymorphism (AMP-FLP) analysis, has shown promise as a means of analyzing forensic biological evidence. A variety of DNA extraction methods were evaluated for their suitability for AMP-FLP analysis.
CT Comey, BW Koons, KW Presley, JB Smerick, CA Sobieralski, DM Stanley, FS Baechtel   +6 more
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