Results 101 to 110 of about 352,913 (253)

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Analytical Quality by Design (AQbD) Approach to the Development of In Vitro Release Test for Topical Hydrogel. [PDF]

Pharmaceutics, 2022
Szoleczky R, Budai-Szűcs M, Csányi E, Berkó S, Tonka-Nagy P, Csóka I, Kovács A.   +6 more
europepmc   +1 more source

Quality by Design and Analytical Quality by Design in Pharmaceutical Analytical Method Development: Principles, Tools, and Regulatory Perspectives

A methodical, risk- and science-based approach to pharmaceutical development, Quality by Design (QbD) places an emphasis on incorporating quality into products rather than depending just on end-product testing. It entails creating a Design Space backed by risk assessment and statistical tools like Design of Experiments (DoE), as well as defining the ...
openaire   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Analytical quality-by-design approach for development and validation of HPLC method for the simultaneous estimation of omarigliptin, metformin, and ezetimibe: application to human plasma and dosage forms. [PDF]

BMC Chem, 2023
Magdy G, Al-Enna AA, Belal F, El-Domany RA, Abdel-Megied AM.   +4 more
europepmc   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

AQbD-Based UPLC-ELSD Method for Quantifying Medium Chain Triglycerides in Labrafac™ WL 1349 for Nanoemulsion Applications

Molecules
In response to recent regulatory guidelines, including ICH (International Council for Harmonisation) Q2 (R2) and Q14, we developed a UPLC-ELSD method to quantify Medium-Chain Triglycerides (MCTs) in Labrafac™ WL 1349 for nanoemulsion applications.
Alessio Gaggero, Viktoria Marko, Dalibor Jeremic, Carolin Tetyczka, Philippe Caisse, Jesús Alberto Afonso Urich   +5 more
doaj   +1 more source

Determination of Bupropion and Its Impurities via a Chaotropic Chromatography Method Following Analytical Quality-by-Design Principles for Method Development. [PDF]

Pharmaceuticals (Basel), 2022
Gkountanas K, Malenović A, Dotsikas Y.
europepmc   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Development of a quality by design-based ultra-performance liquid chromatography method for the simultaneous estimation of casirivimab and imdevimab with greenness metrics

Green Analytical Chemistry
This study presents the development and optimization of an ultra-performance liquid chromatography method for the simultaneous analysis of casirivimab and imdevimab, guided by the principles of Quality by Design.
Sravanthi Gandu, Kumaraswamy Gandla
doaj   +1 more source