Results 81 to 90 of about 458,876 (308)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

THE CORRELATION ANALYSIS OF THE ANGLE MEASUREMENTS

open access: yesGeodesy and cartography, 2012
The test bench for testing and calibrating the measuring geodetic instruments for flat angle measurements has been created at Vilnius Gediminas Technical University, Institute of Geodesy. This test rig incorporates multiple-angular position determination principles – photoelectric angular encoder, polygon-autocollimator and circular-scale ...
Bručas, Domantas   +2 more
openaire   +4 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

An optical angle sensor based on chromatic dispersion with a mode-locked laser source

open access: yesJournal of Advanced Mechanical Design, Systems, and Manufacturing, 2018
This paper proposes a new optical angle sensor, in which a mode-locked laser is employed as the light source, and chromatic dispersion of a collimator objective is utilized to detect the angular displacement of a target of interest.
Yuki SHIMIZU   +3 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

open access: yesFEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young   +7 more
wiley   +1 more source

Integration of Error Compensation of Coordinate Measuring Machines into Feature Measurement: Part II—Experimental Implementation

open access: yesSensors, 2016
Coordinate measuring machines (CMM) are main instruments of measurement in laboratories and in industrial quality control. A compensation error model has been formulated (Part I).
Roque Calvo   +3 more
doaj   +1 more source

Early‐life high‐fat diet exposure increases Achilles tendon stiffness and induces transcriptomic alterations

open access: yesFEBS Open Bio, EarlyView.
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin   +3 more
wiley   +1 more source

High precision angle calibration for spherical measurement systems [PDF]

open access: yes
The European Synchrotron Radiation Facility (ESRF) located in Grenoble, France is a joint facility supported and shared by 19 European countries. It operates the most powerful synchrotron radiation source in Europe.
Martin, David G.
core  

Aerodynamic force and moment measurement of 10 degrees half-angle cone in JF12 shock tunnel [PDF]

open access: yes, 2017
An aerodynamic force and moment measurement was conducted in JF12 long-testduration detonation-driven shock tunnel of Institute of Mechanics, Chinese Academy of Sciences. The test duration of JF12 is 100–130 ms. The nominal Mach number is 7.0 and
汪运鹏   +5 more
core   +1 more source

Low-Cost Contact Angle Measurement System for QCM Sensor [PDF]

open access: yes, 2017
Hydrophobicity is one of the importance factors in the surface properties of materials. This work presents the development of a low-cost contact angle measurement system based on goniometric measurement using an inexpensive digital camera, tilt control ...
Setyawan P. Sakti   +6 more
core   +4 more sources

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