Results 161 to 170 of about 5,412,710 (314)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Cage sizes for tamarins in the laboratory

, 2004
Provision of active space for captive animals is essential for good welfare.  It effects not only their behaviour but also determines whether there is sufficient room for appropriate environmental enrichment.
Buchanan-Smith, Hannah M, Prescott, Mark J   +1 more
core  

Quantifying ASF Outbreak Dynamics in the Philippines (2019-2023) Using Joinpoint Regression and the Estimated Dissemination Ratio. [PDF]

Animals (Basel)
Castro SJM, Salvador RT, Gundran RS, Garcia JS, Siriyakhun S, Pongsumpan J, Na-Lampang K.   +6 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Gaze that shamed science: Canine defiance and the ethics of experimental physiology. [PDF]

Med Humanit
Hung RYY.
europepmc   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

FEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura, Kengo Furuichi, Hiromitsu Akamatsu, Naoki Yamamoto, Sako Keisuke, Akihiko Koshino, Taichiro Minami, Megumi Oshima, Akinori Hara, Norihiko Sakai, Miho Shimizu, Kenta Takahashi, Minako Yamamura, Shin‐ichi Horike, Takiko Daikoku, Kenichi Harada, Yohei Shinmyo, Hiroshi Kawasaki, Takashi Wada, Yasunori Iwata   +19 more
wiley   +1 more source

Correction: Recombinant RBD-based subunit vaccines incorporating high-frequency mutation sites elicit cross-immunity and robust protection against SARS-CoV-2. [PDF]

Front Microbiol
Frontiers Production Office.
europepmc   +1 more source

Welfare of Animals in the Laboratory [PDF]

Nature, 1950
openaire   +2 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Correction: Transcriptome-wide dynamics of m⁶A methylation in ISKNV and Sinopec chuatsi cells infected with ISKNV. [PDF]

BMC Genomics
Miao Q, Jiang J, Huang S, Gao J, Liu Q, Zheng R, Kang Y, Guo C, He J, Xie J.   +9 more
europepmc   +1 more source