Results 91 to 100 of about 2,238,839 (285)

Cryo‐EM Analysis of Adenovirus Vector Reveals Purification Conditions Leading to Loss of Viral Fiber Proteins and Reduced Infectivity

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Vaccines and gene therapies require efficient delivery of genetic material to host cells. Viral vectors leverage viruses' ability to enter host cells to achieve this. Following the SARS‐CoV‐2 vaccine and novel gene therapies that utilize adenovirus vectors, there has been an increase in the demand for large‐scale production of vectors.
Betsy Widdicombe   +7 more
wiley   +1 more source

Anion exchange resin as support for invertase immobilization

open access: yesRevista de Ciências Farmacêuticas Básica e Aplicada, 2009
<p class="MsoNormal" style="margin: 0cm 0cm 0pt; line-height: normal; text-align: justify; mso-layout-grid-align: none;"> The invertase (EC 3.2.1.26) from Saccharomyces cerevisiae was employed as a model enzyme in the evaluation of the adsorption ...
M. Vitolo, R. R. Ribeiro
doaj  

Nuclease‐Free Scalable Purification of Physalis Mottle Virus‐Like Particles Expressed in Escherichia coli

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Plant virus‐like particles (VLPs) are promising nanotechnology platforms, but their translation requires purification strategies suitable for large‐scale production. Here, we developed a nuclease‐free downstream process for Physalis mottle virus (PhMV) VLPs expressed in Escherichia coli, combining ammonium sulfate precipitation, low‐pH ...
Sean K. Hsu   +3 more
wiley   +1 more source

Anion exchange resin as support for invertase immobilization

open access: yesRevista de Ciências Farmacêuticas Básica e Aplicada, 2005
The invertase (EC 3.2.1.26) from Saccharomyces cerevisiae was employed as a model enzyme in the evaluation of the adsorption capacity of DOWEX-1X8-50®, a basic anion exchange resin, when used as support in enzyme immobilization.
R.R. Ribeiro, M. Vitolo
doaj  

Fabrication of anion exchange membrane: a preliminary study [PDF]

open access: yes, 2001
The objective of the work was to fabricate an anion exchange membrane, which can be used for electrodialysis process. The anion exchange membranes were fabricated by means of solution casting method using polysulfone as the binder and anion exchange ...
Sarmidi, Mohamad Roji   +5 more
core  

Feasibility Assessment of a Single‐pH Connected Polishing Train for Monoclonal Antibody Purification Using Mildly Acidic Protein A Elution and an Acidic‐Window Anion Exchange Membrane

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody downstream processing often requires multiple pH adjustments between Protein A capture and polishing steps. These adjustments increase process complexity and make direct unit‐operation connection more difficult. We evaluated a connected post‐capture polishing block operated at approximately pH 5.0 using mild‐pH Protein A ...
Takaki Aiso   +5 more
wiley   +1 more source

Serotype‐Tailored Midstream and Downstream Operations to Advance the Manufacturing of Adeno‐Associated Viral Vectors

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viruses (AAVs) are the leading vectors for in vivo gene therapy, fueling a growing demand for scalable and robust biomanufacturing processes. Yet, the impact of cell lysis conditions on the performance of midstream and downstream unit operations across serotypes remains poorly characterized.
Arianna Minzoni   +20 more
wiley   +1 more source

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