Results 101 to 110 of about 2,238,839 (285)

Continuous Capture of Recombinant Adeno‐Associated Virus Particles Using Twin‐Column CaptureSMB

open access: yesBiotechnology and Bioengineering, EarlyView.
Continuous twin‐column CaptureSMB enables direct capture of secreted rAAV5 from minimally processed perfusion harvest, bypassing lysis, clarification, and concentration steps. Compared to batch capture, it achieves higher yield (+14.3%), greater productivity (+11.4%), and drastically reduced buffer use (−79.2%), while maintaining critical quality ...
Julia M. Müller   +11 more
wiley   +1 more source

Design principles for vitrimers: Linking dynamic covalent chemistry, network architecture, and engineering performance

open access: yesBulletin of the Korean Chemical Society, EarlyView.
This review examines vitrimer performance from a broader perspective, considering not only dynamic covalent chemistry but also molecular structure, network architecture, and rheological behavior. These factors are discussed in relation to service and processing windows, reprocessing, repair, and engineering applications, providing a multiscale ...
Kyosun Ku, Hyeonuk Yeo
wiley   +1 more source

Structural compatibility between β‐lactams and substrate binding pockets of IMP‐1 and NDM‐1 determines the substrate spectrum

open access: yesBulletin of the Korean Chemical Society, EarlyView.
Chemical structures of three β‐lactam classes and the substrate‐binding pockets (SBPs) of B1 metallo‐β‐lactamases (MBLs) were compared. Structural compatibility between β‐lactams and MBL SBPs correlates with enzymatic activity, providing insights into the substrate spectrum of MBLs and β‐lactam resistance.
Kiwoong Kwak   +8 more
wiley   +1 more source

Separation of hafnium from zirconium by anion exchange

open access: yes, 1961
The results of a systematic survey of the elution of hafnium and zirconium in diluted sulfuric acid, and in solutions of hydrochloric and sulfuric acids in water, are presented.
Hague, John L.; Machlan, Lawrence A.
core   +1 more source

High‐throughput development and scale‐up of recombinant adeno‐associated virus 5 anion‐exchange chromatography for high‐purity capsid separation

open access: yesBiotechnology Progress, EarlyView.
High‐throughput anion‐exchange chromatography process development enables scalable purification of high‐purity recombinant adeno‐associated virus capsids. Abstract Recombinant adeno‐associated virus (rAAV) production commonly yields a high proportion of empty and partially filled capsids, necessitating downstream purification to enrich full capsids ...
Kevin Vera   +8 more
wiley   +1 more source

ANION-EXCHANGE IN LITHIUM ALUMINATE HYDROXIDES

open access: yes, 1989
ANION-EXCHANGE IN LITHIUM ALUMINATE ...
Munish Puri (13085961)   +1 more
core  

DNA concentration in harvest affects retrovirus clearance capabilities of protein A chromatography

open access: yesBiotechnology Progress, EarlyView.
Abstract Protein A chromatography is a key unit operation in biopharmaceutical purification, widely used to capture monoclonal antibodies (mAbs) from harvest cell culture fluid (HCCF). In addition to its primary function, protein A chromatography is also frequently tested for its ability to remove viruses. Although protein A specifically binds mAbs and
Lukas Döring   +7 more
wiley   +1 more source

Partial Purification of Viruses with an Anion Exchange Resin.

open access: yesExperimental Biology and Medicine, 1950
A variety of techniques have been developed for the purification of viruses. Methods which have been applied to poliomyelitis viruses include: adsorption to, and elution from alumina gel1 or aluminum hydroxide2; half saturation with ammonium sulfate3; filtration4; procedures involving ether extraction, protein precipitation and centrifugation or ...
openaire   +2 more sources

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