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Anther Culture in Cucurbita Species

2021
Production of homozygous pure parental lines is the first stage of hybrid vegetable breeding. Unfortunately, producing pure lines takes a long time by classical breeding methods, especially in open-pollinated vegetable species, and this period can be up to 8-10 years.
Ertan Sait, Kurtar, Musa, Seymen
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Barley Anther Culture

2018
The production of doubled haploid (DH) barley plants through anther culture is a very useful yet simple in vitro technique. DH plants derive from divisions of haploid microspores that have undergone a developmental switch under the appropriate conditions. The successive divisions lead to the formation of an embryo or callus rather than the formation of
Ludmila, Ohnoutkova   +2 more
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Float culture of wheat anthers

Theoretical and Applied Genetics, 1981
Experiments on wheat anther culture in liquid media either synthetic or with potato extract show that it is possible to obtain as many embryos as when using solid potato extract medium. In liquid media young embryos or 14-day old induced anthers can differentiate green plants for regeneration. Glutamine is effective in culturing anthers and can replace
Y, Henry, J, De Buyser
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Microspore growth and anther staging in barley anther culture

Plant Cell Reports, 1986
Nuclear growth, microspore cell growth and cell cycle stage were examined in microspores of anthers of Hordeum vulgare L. cv. Klages taken from florets of the middle of the spike as per anther staging methods. Although there was wide variation in nuclear size at all stages of the cell cycle, mean nuclear size appeared to be a good indicator of cell ...
W G, Wheatley   +2 more
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Anther culture of Rosa

Scientia Horticulturae, 1981
Abstract Anthers of 2 tetraploid Rosa species (4 n =28) were plated on selected basis media, supplemented with various amounts of auxins and kinetin, at different bud stages and using various light conditions. Among the media tested, MS medium with 2.0 mg/l IAA and 0.4 mg/l K was generally the best for anther culture of R.
Z. Tabaeezadeh, M. Khosh-Khui
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Oak anther culture

2003
Oaks (Quercus spp.) are widely distributed trees of temperate regions, and are of major importance in forestry. Most species are used for wood production, although there are a few interesting peculiarities, such as cork production from the cork oak.
M. A. Bueno, J. A. Manzanera
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Oat (Avena sativa L.) Anther Culture

2021
Production of doubled haploids (DHs) by androgenesis is a promising and convenient alternative to traditionally used breeding techniques. Low response of anther culture and strong genotype dependency in the development of embryo-like structures (ELS) was reported for oat (Avena sativa L.). Total homozygosity has been reached in one generation.
Marzena, Warchoł   +5 more
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Triticale anther culture

2003
The present long-term tissue culture programme aims at directing double haploid (DH) production of triticale towards responsive genotypes and at the same time allowing the practical use of anther culture in current breeding programmes. It is hoped that this can be achieved without limiting plant breeders to a few selected crosses.
S. Tuvesson, R. von Post, A. Ljungberg
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Wheat anther culture

2003
The following procedure for large-scale production of doubled haploids (DH) in wheat at Svalof Weibull AB (SW) is based on protocols developed in Sven Bode Andersen’s laboratory in Copenhagen (Andersen et al., 1987; Tuvesson et al.,1989). Since then the tissue culture programmes were rationalised to a few steps in order to speed up DH production and ...
S. Tuvesson, R. von Post, A. Ljungberg
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