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Anther Culture of Potato: Method and Applications
2021Anther culture provides a tool to produce haploid lines from cultivated potato (Solanum tuberosum L.), which has a tetraploid (2n = 4x = 48) genome constitution. Shoot regeneration via direct embryogenesis in anther culture procedure is preferred to produce dihaploid (2n = 2x = 24) potato lines, which can be applied in breeding of potato varieties. The
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2003
The production of doubled haploid (DH) barley (Hordeum vulgare L.) plants has proven to be a highly valuable tool for plant breeding, allowing the release of a high number of new cultivars (Devaux et al., 1996). Doubled haploids have also been fundamental for genetic analysis, linkage maps production and QTL analysis in barley.
L. Cistué +4 more
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The production of doubled haploid (DH) barley (Hordeum vulgare L.) plants has proven to be a highly valuable tool for plant breeding, allowing the release of a high number of new cultivars (Devaux et al., 1996). Doubled haploids have also been fundamental for genetic analysis, linkage maps production and QTL analysis in barley.
L. Cistué +4 more
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1984
In this chapter, the published reports of anther and microspore culture of Petunia will be reviewed in comparison with culture of related Solanaceous genera. The reasons for the type and frequency of microspore development in Petunia will be explored.
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In this chapter, the published reports of anther and microspore culture of Petunia will be reviewed in comparison with culture of related Solanaceous genera. The reasons for the type and frequency of microspore development in Petunia will be explored.
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Anther culture of Anthurium: a review
Acta Physiologiae Plantarum, 2015The use of anther culture to produce haploid plants is a useful breeding technique for Anthurium, but has only seen limited applications thus far. This review describes the advances achieved thus far, and provides practical tips and protocols that would allow researchers new to the field, or already experienced Anthurium tissue culture scientists, to ...
Jaime A. Teixeira da Silva +3 more
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Pollen dimorphism and anther culture in barley
Planta, 1975A dimorphism is observed in barley (Hordeum vulgare L., cv. Akka) pollen when stained with acetocarmine from the mid-binucleate stage onwards. The majority of grains have staining cytoplasms, while the remainder have cytoplasms which take up little or no stain (NS grains).
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Anther and microspore culture in tobacco
2003Serving as the oldest model species, several variants of tobacco anther and microspore culture have been developed over the years, depending on the timing of the inductive stress treatment and microspore isolation (Heberle-Bors et al., 1996). A convenient, one-step procedure of anther culture in Nicotiana tabacum consists of a cold-treatment of excised
A. Touraev, E. Heberle-Bors
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Anther culture of papaya (Carica papaya L.)
Plant Cell Reports, 1985Papaya (Carica papaya L.) anther containing microspores in tetrad to early-binucleate stages were successfully cultured on 1/2 strength MS salts and vitamins with full strength Na-Fe-EDTA supplemented with 2 mg/l NAA, 1 mg/l BA and 6% sucrose for callus initiation and formation.
H S, Tsay, C Y, Su
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Protocol for rye anther culture
2003Rye (Secale cereale L.) is a cross-pollinated cereal, which has a strong self-incompatibility system. Population varieties of rye are highly heterozygous. Genes for self-fertility can, however, be introduced from wild relatives for purposes of hybrid rye breeding.
Immonen, Sirkka, Tenhola-Roininen, Teija
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Anther Culture Protocols for Barley and Wheat
Doubled haploid (DH) techniques remain valuable tools for wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) genetic improvement, and DH populations are used extensively in breeding and research endeavors. Several techniques are available for DH production in wheat and barley.Sue, Broughton +4 more
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Anther Culture and Production of Haploids
1998Haploid plants have the gametophytic number of chromosomes. They can be derived by several different ways. Haploids via twin seedlings can spontaneously occur in nature. Maternal haploids may arise from certain interspecific crosses followed by the elimination of chromosomes from the pollen donor during embryo development.
Germán Spangenberg +2 more
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