Results 281 to 290 of about 1,405,286 (325)

Localization of anti-nuclear antibodies on human metaphase chromosomes

Journal of Immunological Methods, 1974
Abstract A technique has been developed for the in vitro demonstration of anti-nuclear antibodies (ANA) in the sera of patients with systemic lupus erythematosus (SLE) utilizing human metaphase chromosomes in situ as substrate. The selective removal of histone, acidic protein, RNA and treatment with 2% formamide improves the binding of antibodies to ...
J.I. Watson, R.B. Church, Marvin J. Fritzler   +2 more
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New Methods for Detection of Anti-nuclear Antibodies

Clinical Immunology and Immunopathology, 1998
Many different autoantibodies which react with a variety of different nuclear and cytoplasmic antigens have been described. Detection of some these antibodies has been shown to be clinically useful in a number of different autoimmune diseases.
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Demonstration of anti‐nuclear antibodies in fowl sera by immunofluorescence

Avian Pathology, 1980
The occurrence of anti-nuclear antibodies in fowl sera is described. The anti-nuclear activity was demonstrated by immunofluorescence in a small percentage (3.9%) of the sera tested. The nuclei of cultured fowl and turkey cells were stained by anti-nuclear serum whereas the nuclei of several mammalian tissues were not.
V.M. Calvert, B.M. Adair
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Methods for detection and characterization of anti-nuclear antibodies

Clinical Rheumatology, 1990
The detection and characterization of ANA can actually be done in most clinical laboratories using commercially available ready-to-use reagents. However when chosing a kit great care should be taken in the selection of standardized preparations previously tested with a large reference panel of sera, including a maximum of ANA patterns as well as ...
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Experimental studies on the mechanism of induction of anti-nuclear antibodies by procainamide

Clinical Immunology and Immunopathology, 1977
Abstract The occurrence of anti-nuclear factor (ANF) following immunization with procainamide (PrA) conjugates, reactions in vitro between PrA and nuclear components, and the effect of PrA on the immune response to soluble nucleoprotein (SNP) and DNA were examined.
Shlomo Ben-Efraim, Zeev Steiner, A. Faivisewitz, Abraham Klajman, Eugene F. Gold   +4 more
openaire   +3 more sources

Protein-losing enteropathy associated with hypocomplementemia and anti-nuclear antibodies

Journal of Gastroenterology, 2000
We report a case of protein-losing enteropathy associated with an autoimmune disorder, presumably systemic lupus erythematosus. Although typical manifestations of systemic lupus erythematosus were lacking, a high serum cholesterol level, a low serum complement level, positivity for anti-nuclear antibody, and positivity for anti-single-strand DNA ...
Atsushi Suzuki, Atsushi Nakajima, Kanae Kubo, Toshihide Mimura, Yoshio Yazaki, Shin Ohnishi, Nobuyuki Matsuhashi   +6 more
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Anti-nuclear antibodies and the optic-spinal form of multiple sclerosis

Journal of Neurology, 1997
We found anti-nuclear antibodies (ANA) (1:20 or higher) in sera from 11 of 16 patients with a diagnosis of the optic-spinal form of multiple sclerosis (OpS-MS) and 13 of 59 patients with other forms of MS (other MS), both rates being significant (P = 0.0004). Six of the OpS-MS patients had a high level of ANA (1:80 or higher), while levels were high in
Hidenao Sasaki, Kunio Tashiro, Seiji Kikuchi, Kazuo Miyasaka, T. Fukazawa, Takeshi Hamada, Koji Hamada   +6 more
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Anti‐nuclear matrix antibodies in mixed connective tissue disease

European Journal of Immunology, 1982
AbstractPurified serum antibodies of patients suffering from mixed connective tissue disease were tested for their immunological specificity against nuclear constituents of HeLa S3 cells. In the indirect immunofluorescent staining technique, using cells and nuclei as targets, a typical speckled intranuclear staining pattern was obtained, that persisted
Gertie Vierwinden, Martin Salden, Walther J. van Venrooy, W. J. Habets, Chris A.G. van Eekelen, Leo B A van de Putte   +5 more
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A semiquantitative measurement of anti-nuclear antibody using immuno-microfluorimetry

Archives of Dermatological Research, 1979
1. Using microfluorimetry, the strength of fluorescence was measured on fluoresceinated anti-nuclear antibody of SLE sera. The indirect "Sandwich" method was applied using human peripheral lymphocytes as substrates tissue. 2. The results of using three FITC-labelled anti-human IgG conjugates of different types were compared with each other. 3.
Itaru Yoskii, Hiroaki Ueki, Nozomi Nohara, Akira Ikeda   +3 more
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Sperm auto - antibodies and anti-nuclear antigen antibodies in chronic dioxin - exposed veterans

Chemosphere, 1996
The authors studied anti-nuclear antibodies (ANA) in 25 chronic dioxin - exposed veterans by IIF technics with Hep-2 cell line and sperm autoantibodies by agglutination test of Franklin-Dukes. The site of antibody binding on spermatozoon is detected by IIF test. The control group for ANA detection is 63 healthy persons of the same age as that of dioxin
Tran Thi Chinh, Phan Thi Phi Phi, Nguyen Thanh Thuy   +2 more
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