Results 31 to 40 of about 119,543 (251)

Drug-Resistant Helicobacter pylori: Diagnosis and Evidence-Based Approach

Diagnostics, 2023
Helicobacter pylori (H. pylori) is the most common chronic bacterial infection, affecting approximately half of the world’s population. H. pylori is a Class I carcinogen according to the World Health Organization, and the International Agency for ...
Vaneet Jearth, Mitali Madhumita Rath, Abhirup Chatterjee, Aditya Kale, Manas Kumar Panigrahi   +4 more
doaj   +1 more source

The microenvironment in antibiotic susceptibility testing

APMIS
Antibiotic susceptibility testing (AST) by agar diffusion has been repeatedly standardized and, in most cases, gives results which predict clinical success when antibiotic treatment is based on such results. The formation of the inhibition zone is due to a transition from planktonic to biofilm mode of growth.
Niels Høiby, Claus Moser, Oana Ciofu
openaire   +2 more sources

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Results of bacterial cultivation are infrequently utilized in the treatment of patients hospitalized with severe odontogenic infections – a retrospective cohort study

Journal of Oral Microbiology
Background Patients hospitalized with severe odontogenic infections (SOI) receive empiric intravenous antibiotics. Microbiological cultivation and antibiotic susceptibility testing are commonly performed, although the clinical value is debated.Objective ...
Rasmus Søndenbroe, Merete Markvart, Daniel Belstrøm, Frederik Boëtius Hertz, Thomas Bjarnsholt, Claus Henrik Nielsen, Sanne Werner Møller Andersen, Simon Storgård Jensen   +7 more
doaj   +1 more source

Simple Sample Preparation Method for Direct Microbial Identification and Susceptibility Testing From Positive Blood Cultures

Frontiers in Microbiology, 2018
Rapid identification and determination of the antibiotic susceptibility profiles of the infectious agents in patients with bloodstream infections are critical steps in choosing an effective targeted antibiotic for treatment.
Hong-wei Pan, Wei Li, Rong-guo Li, Yong Li, Yi Zhang, En-hua Sun   +5 more
doaj   +1 more source

New Method for Antibiotic Susceptibility Testing [PDF]

Antimicrobial Agents and Chemotherapy, 1972
The most widely used method for routine antibiotic susceptibility testing of clinical isolates of bacteria is the paper-disc method. This has the advantage of simplicity, but to obtain reliable results considerable care is required both in the standardization of the procedure and in the interpretation of the zones of inhibition.
G N, Rolinson, E J, Russell
openaire   +2 more sources

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Antibiotic Susceptibility Testing of Bacteroides [PDF]

Antimicrobial Agents and Chemotherapy, 1972
Seventy Bacteroides strains isolated from clinical materials were tested in vitro against nine antibiotics by means of modified disc diffusion and agar dilution tests. Correlating the agar dilution susceptibility with the concentrations achievable in serum for each drug tested, we found that at least 90% of the ...
S J, Bodner, M G, Koenig, L L, Treanor, J S, Goodman   +3 more
openaire   +2 more sources

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source