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Bacterial Expression of Anti-DNA Antibody Domains
Methods, 1997Bacterial production of recombinant Fab or Fv domains of antibodies is an important tool for analyzing structural correlates of antigen binding or idiotype expression. Bacterial products may be Fab or Fv molecules that assemble from separate chains in the periplasm or a single-chain Fv protein.
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Expression of Single-Domain Antibodies in Bacterial Systems
2012In this chapter we describe in detail the current protocols that are used to express single-domain antibodies in bacteria. Bacteria are among the most common expression systems for expressing recombinant proteins. We present different approaches for carrying out periplasmic and cytoplasmic expression, as well as small-scale and large-scale expression ...
Baral, T.N., Arbabi-Ghahroudi, M.
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Antibody Immunotherapy of Gram‐negative Bacterial Sepsis
Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy, 1987Gram‐negative bacterial sepsis continues to represent a significant cause of morbidity and mortality in hospitalized patients. Currently available medical therapy (antimicrobial agents, hemodynamic monitoring, aggressive fluid resuscitation, and nutritional support) for this disease process has reduced but not eliminated the severe
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Anti-bacterial monoclonal antibodies: Back to the future?
Archives of Biochemistry and Biophysics, 2012Today's medicine has to deal with the emergence of multi-drug resistant bacteria, and is beginning to be confronted with pan-resistant microbes. This worsening inadequacy of the antibiotics concept, which has ruled infectious medicine in the last six decades creates an increasing unmet medical need that can be addressed by passive immunization.
Martin B, Oleksiewicz +2 more
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Epitope mapping of antibodies using bacterial surface display
Nature Methods, 2008We describe a method for mapping the epitopes recognized by antibodies, based on bacterial surface expression of antigen protein fragments followed by antibody-based flow-cytometric sorting. We analyzed the binding sites of both monoclonal and polyclonal antibodies directed to three human protein targets: (i) the human epidermal growth factor receptor ...
Johan, Rockberg +4 more
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Antibody screening of bacterial expression systems
1995Abstract Screening expression libraries with specific antibodies is a well-established method for the identification of cDNA clones (1, 2). It is often used when an accurate protein sequence is not available, thus precluding the design of appropriate oligonucleotide probes.
J Paul Luzio +3 more
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A Bacterial IgG-Degrading Enzyme to Unhinge Antibodies
New England Journal of Medicine, 2017The number of patients awaiting renal transplantation heads ever upward (in the United States alone, more than 105,000 patients are currently on the waiting list for a kidney from a deceased donor, according to the United Network for Organ Sharing), and many of those patients are unlikely to receive kidneys, because they have preformed antibodies to ...
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Bacterial resistance to antibodies: a model evolutionary study
Journal of Theoretical Biology, 2017The tangled nature model of evolution (reviewed in the main text) is adapted for use in the study of antibody resistance acquired by horizontal gene transfer. Exchanges of DNA and the acquisition of resistant gene sequences are considered. For the parameters used, resistant strains rapidly proliferate and dominate, although initial intense antibiotic ...
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Integrative oncology: Addressing the global challenges of cancer prevention and treatment
Ca-A Cancer Journal for Clinicians, 2022Jun J Mao,, Msce +2 more
exaly
Monoclonal antibodies for bacterial infection
Clinical Immunology Newsletter, 1983Richard A. Polin, Mary Catherine Harris
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