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ANTIBODIES AS INDICATORS FOR BACTERIAL SURFACE STRUCTURES
Annual Review of Microbiology, 1956Antibodies have been used very extensively for the detection, isolation, and identification of the ceIJular components of bacteria. This work could be applied, however, only in part to an evaluation of the topography of immuno chemically defined components in the cellular architecture.
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Enzymatic Antibody Modification by Bacterial Transglutaminase
2013Enzymatic posttranslational modification of proteins permits more precise control over conjugation site than chemical modification of reactive amino acid side chains. Ideally, protein modification by an enzyme yields completely homogeneous conjugates with improved properties for research or therapeutic use. As an example, we here provide a protocol for
Roger Schibli+2 more
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Measuring Antibody Orientation at the Bacterial Surface
2016Many bacteria have the ability to interact with antibodies as a means to circumvent the immune response. This includes binding to the Fc portion of antibodies, effectively reversing the antibody orientation and thus decreasing the Fc-mediated immune signaling.
Oonagh Shannon, Pontus Nordenfelt
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Use of antibodies for analysis of bacterial proteins
Journal of Microbiological Methods, 1993Abstract The specificity of antibody molecules has proved to be of great value for serological identification and classification of bacteria. In the research laboratory monoclonal and polyclonal antibodies have been used extensively for comparative studies of proteins expressed by related organisms, as well as for the fine mapping of epitopes on ...
Ervin L. Faulmann+2 more
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Role of Antibodies in Bacterial Sepsis [PDF]
This symposium has focused on many of the biological manifestations and late consequences of sepsis. Several contributors have tried to define sepsis as a chemically distinct syndrome; many have sought mediators of the tissue damage triggered by it.
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Antibody recognition of bacterial surfaces and extracellular polysaccharides
Current Opinion in Structural Biology, 2020Because of the ongoing increase in antibiotic-resistant microbes, new strategies such as therapeutic antibodies and effective vaccines are required. Bacterial carbohydrates are known to be particularly antigenic, and several monoclonal antibodies that target bacterial polysaccharides have been generated, with more in current development.
Paul A. Ramsland+4 more
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Expression of Single-Domain Antibodies in Bacterial Systems
2012In this chapter we describe in detail the current protocols that are used to express single-domain antibodies in bacteria. Bacteria are among the most common expression systems for expressing recombinant proteins. We present different approaches for carrying out periplasmic and cytoplasmic expression, as well as small-scale and large-scale expression ...
Mehdi Arbabi-Ghahroudi+3 more
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Antibody responses to bacterial antigens in the pregnant ewe
New Zealand Veterinary Journal, 1986The humoral immune response to the novel antigen Brucella abortus (S19) was examined in nonpregnant ewes and in ewes at different stages of pregnancy. Both the primary and secondary responses were monitored, by measuring the levels of total antibody, Immunoglobulin G (IgG), and complement fixing antibody.
J.F.T. Griffin, G.E. Reynolds
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Arthritis & Rheumatism, 2008
OBJECTIVE To map the antibody response to human citrullinated alpha-enolase, a candidate autoantigen in rheumatoid arthritis (RA), and to examine cross-reactivity with bacterial enolase.
K. Lundberg+7 more
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OBJECTIVE To map the antibody response to human citrullinated alpha-enolase, a candidate autoantigen in rheumatoid arthritis (RA), and to examine cross-reactivity with bacterial enolase.
K. Lundberg+7 more
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Bacterial lysates in the prevention of respiratory tract infections.
Otolaryngologia polska = The Polish otolaryngology, 2018Bacterial lysates stimulate the general immunity of the body in a non-specific way. They act on non-specific defense mechanisms, leading to an increase in type A antibody in mucous membranes, phagocytic activity and INF-ƴ production.
D. Jurkiewicz, B. Zielnik-Jurkiewicz
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