Results 71 to 80 of about 932,790 (307)
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Understudied Factors Influencing Fc-Mediated Immune Responses against Viral Infections
Vaccines, 2019 Antibodies play a crucial role in host defense against viruses, both by preventing infection and by controlling viral replication. Besides their capacity to neutralize viruses, antibodies also exert their antiviral effects by crystallizable fragment (Fc)-
Sai Priya Anand, Andrés Finzi
doaj +1 more source
DETECTION OF THE BOVINE VIRAL DIARRHEA ANTIBODIES [PDF]
Biotechnologia Acta, 2013 Bovine viral diarrhea is a widespread infection of cattle that has a wide range of clinical symptoms in domestic and wild ruminants. It is a major problem in cattle and causes significant economic losses in the cattle industry.
I. V. Goraichuk +5 more
doaj +1 more source
Role of early viral surface antigens in cellular immune response to vaccinia virus [PDF]
, 1976 Infection of mice with the vaccinia virus strain WR, Elstree or DIs, a conditional lethal mutant of vaccinia virus, resulted in the generation of vaccinia virus-specific sensitized cytolytic T lymphocytes (CTL).
Ertl, H., Koszinowski, Ulrich H.
core +1 more source
The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells
FEBS Letters, EarlyView.The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman +3 more
wiley +1 more source
VirScan: High-throughput Profiling of Antiviral Antibody Epitopes
Bio-Protocol, 2022 Profiling the specificities of antibodies can reveal a wealth of information about humoral immune responses and the antigens they target. Here, we present a protocol for VirScan, an application of the phage immunoprecipitation sequencing (PhIP-Seq ...
Ellen Shrock +2 more
doaj +1 more source
Application of I131 to Determination of Viral Antibodies
Experimental Biology and Medicine, 1955 SummaryExperiments are described in which preparations of yellow fever virus, Bwamba fever virus and influenza virus were allowed to react with corresponding antisera labeled with radioactive iodine (I131). No significant differences were observed in the activity of the sediments obtained with the first 2 virus systems from those of appropriate ...
Delphine H. Clarke, Francis L. Black
openaire +3 more sources
A nucleotide‐independent, pan‐RAS‐targeted DARPin elicits anti‐tumor activity in a multimodal manner
Molecular Oncology, EarlyView.We report a Designed Ankyrin Repeat Protein that binds and inhibits RAS proteins, which serve as central cell signaling hubs and are essential for the progression of many cancers. Its unique feature is that it does not discriminate between different RAS isoforms or mutations and is capable of binding to RAS in both its active (GTP‐bound) and inactive ...
Jonas N. Kapp +13 more
wiley +1 more source
Antibody-mediated prevention and treatment of HIV-1 infection
Retrovirology, 2018 Novel broadly neutralizing antibodies targeting HIV-1 hold promise for their use in the prevention and treatment of HIV-1 infection. Pre-clinical results have encouraged the evaluation of these antibodies in healthy and HIV-1-infected humans.
Henning Gruell, Florian Klein
doaj +1 more source
A SARS-CoV-2 variant elicits an antibody response with a shifted immunodominance hierarchy.
PLoS Pathogens, 2022 Many SARS-CoV-2 variants have mutations at key sites targeted by antibodies. However, it is unknown if antibodies elicited by infection with these variants target the same or different regions of the viral spike as antibodies elicited by earlier viral ...
Allison J Greaney +13 more
doaj +1 more source